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Plasmid#80638PurposeResidues 205-222 of CHMP4A, Mutation disrupts binding to ALIX; Bacterial Expression Vector; TEV-cleavable GST-tag; Sundquist Lab Internal ID: WISP06-204DepositorInsertCHMP4A (CHMP4A Human)
UseTagsGSTExpressionBacterialMutationResidues 205-222; Changed Leucine 217 to AlaninePromoterAvailable sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
GST-CHMP4A (205-222) W220A (pGEX2T(TEV))
Plasmid#80637PurposeResidues 205-222 of CHMP4A, Mutation disrupts binding to ALIX; Bacterial Expression Vector; TEV-cleavable GST-tag; Sundquist Lab Internal ID: WISP06-203DepositorInsertCHMP4A (CHMP4A Human)
UseTagsGSTExpressionBacterialMutationResidues 205-222; Changed Trp 220 to AlaPromoterAvailable sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
GST-CHMP4A (205-222) L214A (pGEX2T(TEV))
Plasmid#80639PurposeResidues 205-222 of CHMP4A, Mutation disrupts binding to ALIX; Bacterial Expression Vector; TEV-cleavable GST-tag; Sundquist Lab Internal ID: WISP06-205DepositorInsertCHMP4A (CHMP4A Human)
UseTagsGSTExpressionBacterialMutationResidues 205-222; Changed Leucine 214 to AlaninePromoterAvailable sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pmirGlo-CALB2-3'UTR delta ARE
Plasmid#74425PurposeCalb2 UTR with mutated AU binding sequenceDepositorInsertCALB2 3'UTR (CALB2 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
mOC-STAMP (N162D)_3xFlag-Myc-CMV26
Plasmid#73578PurposeExpress mouse OC-STAMP ((N162D) in mammalian cellsDepositorInsertOC-STAMP (Ocstamp Mouse)
UseTags3x Flag and c-Myc tagExpressionMammalianMutationchanged asparagin (N) 162 to Aspartic Acid (D)PromoterAvailable sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUAST-HA, Pbl-A C-Term
Plasmid#69795PurposePebble isoform A, C-Term, in P element-based pUAST vector for Gal4-regulated expression in Drosophila.DepositorInsertPebble C-Term domain (Drosophila guanine nucleotide exchange factor)
UseP element-based puast vector for gal4-regulated e…TagsHA-TagExpressionInsectMutationJust the C-Term domain of Pebble (pbl)Promoterhsp70 promoterAvailable sinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQCXIB-FLAG-hDIXDC1-L-S592A
Plasmid#61225PurposeRetrovirus expressing long isoform of human DIXDC1DepositorInserthuman DIXDC1 (DIXDC1 Human)
UseRetroviralTagsFLAGExpressionMutationChanged Serine 592 to AlaninePromoterAvailable sinceMarch 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENTR-TER+-shDIXDC1-Ms-58
Plasmid#61236PurposeEntry clone encoding shRNA to mouse DIXDC1DepositorInsertDixdc1 (Dixdc1 Mouse)
UseEntr cloneTagsExpressionMutationPromoterH1/TOAvailable sinceMarch 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENTR-TER+-shDIXDC1-Ms-57
Plasmid#61235PurposeEntry clone encoding shRNA to mouse DIXDC1DepositorInsertDixdc1 (Dixdc1 Mouse)
UseEntr cloneTagsExpressionMutationPromoterH1/TOAvailable sinceMarch 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Hygro-FLAG-hDIXDC1 S592A
Plasmid#61214PurposeRetrovirus expressing long isoform of human DIXDC1DepositorInserthuman DIXDC1 (DIXDC1 Human)
UseRetroviralTagsFLAGExpressionMutationChanged Serine 592 to AlaninePromoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLentiX2-PURO-shDIXDC1-Ms-58
Plasmid#61233PurposeLentivirus expressing shRNA to mouse DIXDC1DepositorInsertDixdc1 (Dixdc1 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-Blast-FLAG-hDIXDC1 S592A
Plasmid#61221PurposeLentivirus expressing long isoform of human DIXDC1DepositorInserthuman DIXDC1 (DIXDC1 Human)
UseLentiviralTagsFLAGExpressionMutationChanged Serine 592 to AlaninePromoterPGKAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV/TO-Puro-FLAG-hDIXDC1 S592A
Plasmid#61219PurposeLentivirus expressing long isoform of human DIXDC1DepositorInserthuman DIXDC1 (DIXDC1 Human)
UseLentiviralTagsFLAGExpressionMutationChanged Serine 592 to AlaninePromoterCMV/TOAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
rAAV2-retro helper
Plasmid#81070Purposeretrograde AAV helper vector, carrying rep and modified capsid geneDepositorInsertsrep
rAAV2-retro cap
UseAAV and Unspecified; Helper vectorTagsExpressionMutationPro2Ala, possibly Asp613Gly and changes made to A…PromoterAvailable sinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-FuG-B2
Plasmid#67513PurposeThis is an envelope gene encoding plasmid to make retrogradely lentiviral vector. You can make type B2 envelope coating virus particle with HiRet by using this plasmid.DepositorInsertFusion Glycoprotein type B2
UseLentiviralTagsFusion protein of Vesicular stomatitis Indiana vi…ExpressionMutationPromoterCAGGSAvailable sinceJuly 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL403
Plasmid#119953PurposeExpresses GPPS and LimS, for the production of GPP and (S)-limonene, in Escherichia coli.DepositorInsertsgpps
limS
UseTagsExpressionBacterialMutationN-terminal truncation of 56 amino acid residues (…PromoterAvailable sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPPI4-Strep-SNAP-CTLA-4-His6
Plasmid#223583PurposeProtein purification of human CTLA4 with Twin-Strep, SNAP, GCN4 and 6xHis tag from mammalian cellsDepositorInsertCTLA4 (CTLA4 Human)
UseTagsHis6 and Twin-Strep, SNAPExpressionMammalianMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAF92
Plasmid#135929PurposeAll-in-one Sleeping Beauty vector for Expression for Tasmanian devil mCherry-TNFSF9 (type II transmembrane) fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFSF9).DepositorInsertmCherry fused to TNFSF9
UseTransposonTagsmCherryExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
MLRV
Plasmid#118069PurposeMulti-luciferase reporter vector including transcriptional reporters for NF-kb, TGF-b, c-Myc, p53 and MAPK/JNK transcriptional reportersDepositorInsertsTB:5xNF-κβ:RedF:bGHpA
TB:4xTGF-β:FLuc:bGHpA
TB:5xE-box:Renilla:bGHpA
TB:2xp53:NLuc:bGHpA
TB:6xAP-1:GrRenilla:bGHpA
CMV:ELuc:bGHpA
UseLuciferase and Synthetic Biology; Assembly of gb2…TagsExpressionMammalianMutationPromoter2xp53::miniP, 4xTGF-B::miniP, 5xEbox::miniP, 5xNF…Available sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-ATF4 mScarlet NLS
Plasmid#115969PurposeER-stress sensor - ATF4 translation at ORF3 fluorescent reporter with mScarlet-IDepositorInsertactivating transcription factor 4 (ATF4 Human, Synthetic)
UseLentiviralTagsHA tag, c-myc NLS, and mScarlet-IExpressionMammalianMutationPromoterCMVAvailable sinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only