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Plasmid#174091PurposeCMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a C-terminal bi-partite NLS, 3x flag tag, and P2A-mScarletDepositorInserthuman codon optimized SpCas9 with BPNLS-3xFLAG-P2A-mScarlet
UseIn vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-mScarletExpressionMammalianMutationPromoterpCMV and T7Available sinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAC1818_pCR8-gRG6-SA
Plasmid#118652PurposeTransient transfection; Expressed RG6-SA CasRx gRNA; Gateway DonorDepositorInsertgRG6-SA
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHEY1.1.0-gDNA
Plasmid#112393PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor HEY1DepositorInsertHEY1 (HEY1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBzCas13b
Plasmid#164862PurposeInducible expression of BzCas13b nuclease in bacteria.DepositorInsertBergeyella zoohelcum Cas13b
UseTagsExpressionBacterialMutationPromoterT7 PromoterAvailable sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCJH031_proD_LbCas12a-gRNA-GFP_CAM
Plasmid#183075PurposePlasmid expressing Cas12a guide RNA targeting GFP (1) for in vivo interference assay in bacteria.DepositorInsertLbCas12a guide RNA targeting GFP
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
Construct 7 - CMVp-Cas9-3xNLS-HSVpA
Plasmid#81247PurposeExpresses Cas9 fused to 3xNLS driven by CMV promoter. For mammalian cell expressionDepositorInsertCas9-3xNLS
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCD296.7
Plasmid#113320PurposedCas9, MCP-TetD, J107 scRNA.b1DepositorInsertdCas9, MCP-TetD, J107 scRNA.b1_MS2
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDDIT3.1.0-gDNA
Plasmid#112396PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor DDIT3DepositorInsertDDIT3 (DDIT3 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTFAP2C.1.0-gDNA
Plasmid#113792PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor TFAP2CDepositorInsertTFAP2C (TFAP2C Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceApril 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pdCas9- AsiA_m1.1
Plasmid#158064PurposeExpressing evolved CasTA of dCas9 fused AsiA variant 1.1DepositorInsertdCas9-AsiA_m1.1
UseTagsExpressionBacterialMutationPromoterTetOAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLsCas13a-gRNA-NT
Plasmid#164857PurposeConstitutive expression of single-spacer CRISPR array with non-targeting spacer for LsCas13a in bacteria.DepositorInsertLsCas13a-gRNA-NT
UseTagsExpressionBacterialMutationPromoterJ23119Available sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCF525-AcrIIA13b_gb76_GF31-2-hom_lenti
Plasmid#138310PurposeLenti expression of AcrIIA13bDepositorInsertAcrIIA13b
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCF525-AcrIIA15_gb79_Cand27_lenti
Plasmid#138313PurposeLenti expression of AcrIIA15DepositorInsertAcrIIA15
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCF525-AcrIIA14_gb77_Cand9_lenti
Plasmid#138311PurposeLenti expression of AcrIIA14DepositorInsertAcrIIA14
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBzCas13b-gRNA-NT
Plasmid#164861PurposeConstitutive expression of single-spacer CRISPR array with non-targeting spacer for BzCas13b in bacteria.DepositorInsertBzCas13b-gRNA-NT
UseTagsExpressionBacterialMutationPromoterJ23119Available sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJK504
Plasmid#155381PurposedCas12a crRNAsDepositorInsertdCas12a oscillator crRNAs
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHdCas9-mammalian
Plasmid#138415PurposeExpression of Himar-dCas9 in mammalian cellsDepositorInsertHimar-dCas9
UseTags3xFLAG and SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAEF6
Plasmid#136303PurposepAEF5 plasmid with two gRNA insertedDepositorInsertdouble cassette coding for gRNA
UseCRISPR; Template for pcr to insert double grna co…TagsExpressionMutationPromoterAvailable sinceFeb. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJB166
Plasmid#86998PurposeCRISPR/Cas9 in fission yeast using fluoride selectionDepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCD294.9
Plasmid#113319PurposedCas9, PCP-SoxS_R93A, J109 scRNA.b1DepositorInsertdCas9, PCP-SoxS_R93A, J109 scRNA.b1_PP7
UseCRISPRTagsExpressionBacterialMutationR93APromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only