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Plasmid#135487PurposeAgrobacterium T-DNA with divergent Spun H2A/Bpr driving H2B-tdTomato and hphDepositorInserthph SpunH2A/Bpr H2B-tdTomato
UseTagstdTomatoExpressionBacterial and YeastMutationPromoterSpunH2A/Bpr (divergent)Available sinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMS17
Plasmid#127088PurposeIntegrating vector containing a tetracycline inducible promoter for use in Streptomyces sp.DepositorInsertstcp830
TetRiS
terminator mmrt
Terminator fdt
UseStreptomyce integration vector derived from phic3…TagsExpressionMutationPromoterAvailable sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGI3EM09
Plasmid#135482PurposeAgrobacterium T-DNA with SpunHsp70pr driving hph-GFPDepositorInsertSpunHsp70pr hph-GFP ADH1t
UseTagsGFPExpressionBacterial and YeastMutationPromoterSpunHsp70prAvailable sinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGI3EM18
Plasmid#135484PurposeAgrobacterium T-DNA with SpunH2Bpr driving hph-tdTomatoDepositorInsertSpunH2Bpr hph-tdTomato ADH1t
UseTagstdTomatoExpressionBacterial and YeastMutationPromoterSpunH2BprAvailable sinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEM09
Plasmid#135479PurposeSpizellomyces Hsp70 promoter driving expression of Hygromycin resistance fused to GFPDepositorInsertSpunHsp70pr
UseTagsGFP and hphExpressionBacterial and YeastMutationPromoterSpun Hsp70prAvailable sinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGI3EM22C
Plasmid#135488PurposeAgrobacterium T-DNA with divergent H2A/Bpr driving LifeAct-tdTomato and hphDepositorInserthph SpunH2A/Bpr LifeAct-tdTomato
UseTagsLifeActExpressionBacterial and YeastMutationPromoterSpunH2A/Bpr (divergent)Available sinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGI3EM11
Plasmid#135483PurposeAgrobacterium T-DNA with SpunH2Bpr driving hph-GFPDepositorInsertSpunH2Bpr hph-GFP ADH1t
UseTagsGFPExpressionBacterial and YeastMutationPromoterSpunH2BprAvailable sinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGI3EM29
Plasmid#135489PurposeAgrobacterium T-DNA with Spun H2Bpr driving hph-mCitrineDepositorInserthph SpunH2A/Bpr hph-mCitrine
UseTagsmCitrineExpressionBacterial and YeastMutationPromoterSpunH2A/Bpr (divergent)Available sinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGI3EM30
Plasmid#135490PurposeAgrobacterium T-DNA with Spun H2Bpr driving hph-mClover3DepositorInserthph SpunH2A/Bpr hph-mClover3
UseTagsmClover3ExpressionBacterial and YeastMutationPromoterSpunH2A/Bpr (divergent)Available sinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGI3EM31
Plasmid#135491PurposeAgrobacterium T-DNA with Spun H2Bpr driving hph-mCerulean3DepositorInserthph SpunH2A/Bpr hph-mCerulean3
UseTagsmCerulean3ExpressionBacterial and YeastMutationPromoterSpunH2A/Bpr (divergent)Available sinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMV306DIG13+FFlucRT
Plasmid#49998PurposeIntegrase removed to increase stability of the plasmid in mycobacteriaDepositorInsertG13 promoter + Firefly luciferase Red Thermostable
UseMycobacteria integrating vectorTagsExpressionBacterialMutationCodon optimized for Mycobacterium tuberculosis wi…PromoterG13Available sinceMay 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
Tn7 integration plasmid
Plasmid#154134PurposeSuicidal integration plasmid with the Tn7 arms, streptomycin and kanamycin resistance genes and the restriction site to clone the barcode-carrying cassette.DepositorInsertsleft and right end of Tn7 arm
Spectinomycin resistant gene
UseTagsExpressionBacterialMutationPromoterSpectinomycin resistance gene promoter and noAvailable sinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGM1192
Plasmid#115683PurposeE. coli-Streptomyces shuttle vector for gene expressionDepositorInsertmCherry, aac(3)IV, tsr
UseTagsStrep-tagII on mCherry, codon optimizedExpressionBacterialMutationmCherry codon optimized for StreptomycesPromoterSF14*Available sinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUDP025
Plasmid#103874PurposeBroad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Kluyveromyces lactisDepositorInsertHH-gRNA-HDV targetting ADE2 in Kluyveromyces lactis
UseCRISPRTagsExpressionYeastMutationPromoterScTDH3Available sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUDP082
Plasmid#103875PurposeBroad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Kluyveromyces marxianusDepositorInsertHH-gRNA-HDV targetting ADE2 in Kluyveromyces marxianus
UseCRISPRTagsExpressionYeastMutationPromoterScTDH3Available sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMSG360
Plasmid#27155DepositorTypeEmpty backboneUseMycobacterial ko vectorTagsExpressionMutationPromoterAvailable sinceMarch 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSCBE3
Plasmid#133966PurposeExpresses BE3 CRISPR base editor and gRNA scaffold in StreptomycesDepositorArticleInsertBE3
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLO86
Plasmid#91724Purposemycobacterial expression of mCherry reporterDepositorTypeEmpty backboneUseUnspecified; Mycobacterial expressionTagsmCherryExpressionMutationPromoterAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSCBE2
Plasmid#133965PurposeExpresses BE2 CRISPR base editor and gRNA scaffold in StreptomycesDepositorArticleInsertBE2
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSABEd
Plasmid#133968PurposeExpresses ABEd CRISPR base editor and gRNA scaffold in StreptomycesDepositorArticleInsertABEd
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only