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CMV-SpyCas9
Plasmid#113033PurposeSpyCas9 expression in mammalian cells (CMV promoter driven)DepositorInsertSpyCas9
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTS1051-Tier1-PhCMV-4xCox8
Plasmid#169490PurposeTier-1 vector encoding PhCMV-driven four-fold repeat of a mitochondrial matrix localization signal derived from mitochondrial matrix protein Cox8 (PhCMV-4xCox8-pA)DepositorInsertPCMV-driven mitochondrial localization signal from cytochrome c oxidase subunit 8
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pDN34
Plasmid#61343PurposemCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS2/PKIt NES)DepositorInsertmCherry
UseTagsGS linker-AsLov2-biNLS2 and PKIt NESExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 MEKK1 KD
Plasmid#12180DepositorInsertMEKK1 (Map3k1 Mouse)
UseTagsHis and XpressExpressionMammalianMutationKinase dead: K1253M; V1467LPromoterAvailable sinceSept. 22, 2006AvailabilityAcademic Institutions and Nonprofits only -
pHLuc (Antares-SEPLuc C1A4E+6)
Plasmid#141089Purposefinal optimized bioluminescent reporter for detecting extracellular pH changes in tumor acidosisDepositorInsertAntares-T2A-puromycin-IRESv24-SEP-C1A4E+6
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [AAVS1]
Plasmid#227246PurposeAll-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in HEK293 cellDepositorInsertsTet-On 3G transactivator protein
Enhanced green fluorescent protein
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterEF1α and pTRE3GAvailable sinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-NES-PhoCl-mCherry
Plasmid#87690PurposeExpress NES-PhoCl-mCherry in mammalian cellsDepositorInsertNES-PhoCl-mCherry
UseTagsnuclear export signalExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
SP-GFP-CD274-UTR
Plasmid#121142PurposeMammalian expression of GFP tagged CD274 with its own 3'UTRDepositorInsertCD274 with its own 3'UTR (CD274 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
hHSF1 K80Q
Plasmid#118351PurposePlasmid expresses hHSF1 with K80 in DBD mutated to Q, which abolish DNA-binding activity of HSF1.DepositorInserthHSF1 (HSF1 Human)
UseTagsmyc and 6xHISExpressionMammalianMutationK80QPromoterAvailable sinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP-CBS (WT)
Plasmid#182923PurposeMammalian expression plasmid for human CBSDepositorInsertCystathionine Beta-Synthase (CBS Human)
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
Red Glifon Nega
Plasmid#163117PurposeRed fluorescent protein-based glucose negative control sensor (does not respond to glucose) for mammalian cellsDepositorInsertRed Glifon Nega
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST-ORF-V2
Plasmid#73638PurposeGateway™ destination vector encoding C-terminal fusion with Venus fluorescent protein fragment 2 (V2).DepositorTypeEmpty backboneUseTagsVenus fragment 2 (V2)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
hLOXL2-S300P
Plasmid#200061PurposeLOXL2 mutantDepositorInserthuman lysyl oxidase like 2 (LOXL2 Human)
UseTagsExpressionMammalianMutationS300P mutationPromoterAvailable sinceApril 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSL2783 (pTnsC-VP64_Vch)
Plasmid#200896PurposeExpresses human codon-optimized V. cholerae TnsC-VP64 from a CMV promoter.DepositorInsertVchTnsC-VP64
UseTagsNLSExpressionMammalianMutationPromoterAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST-ORF-V1
Plasmid#73637PurposeGateway™ destination vector encoding C-terminal fusion with Venus fluorescent protein fragment 1 (V1)DepositorTypeEmpty backboneUseTagsVenus fragment 1 (V1)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
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Halo-Cyto
Plasmid#124314PurposeExpresses cytosolic specific HaloTag proteinDepositorInsertHalo tag
UseTagsCytoplasm specific seqExpressionMammalianMutationPromoterCMVAvailable sinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [C12orf35]
Plasmid#227245PurposeAll-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in CHO cellDepositorInsertsTet-On 3G transactivator protein
Enhanced green fluorescent protein
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterEF1α and pTRE3GAvailable sinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only
