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Plasmid#145270PurposeMammalian Expression of Nuclear Opto TBP(53Q) IDR with SV40 NLSDepositorInsertTBP(53Q)-IDR (TBP Human)
UseTagsExpressionMammalianMutationQ-repeat expandedPromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTub-alpha1-EB3-NeonGreen-T2A-iLifeact-mCherry
Plasmid#175261Purposelabelling of microtubule plus end and F-actin structuresDepositorInsertEB3-NeonGreen-T2A-iLifeact-mCherry (MAPRE3 Human)
UseTagsmCherry (on iLifeact) and mNeonGreen (on EB3)ExpressionMammalianMutationNone (wt)PromoterpTub-alpha1Available sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-Flex-DN-AMPK-K45R-T2A-mCherry
Plasmid#83491Purposecre-dependent DN-AMPK under EF1a promoterDepositorInsertDN-AMPK-2A-mCherry
UseAAV and Cre/LoxTagsExpressionMammalianMutationK45RPromoterAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEx1-pEF-H2B-mCherry-T2A-rTetR-HDAC4
Plasmid#78349PurposeExpresses rTetR-HDAC4 for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.DepositorInsertH2B-mCherry-T2A-rTetR-HDAC4 (HDAC4 Human, Synthetic)
UseSynthetic BiologyTagsrTetRExpressionMammalianMutationPromoterpEF alphaAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CamKIIα-CRY2PHR-P2A-CIBN-mCherry-VAMP2
Plasmid#178574PurposeLentiviral vector for CamKIIα::Opto-vTrapDepositorInsertCRY2PHR-P2A-CIBN-mCherry-VAMP2
UseLentiviralTagsExpressionMutationPromoterCaMKIIaAvailable sinceApril 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
P-Ef1a-RPL22-3XFlag-P2A-mCherry-T2A-Puro
Plasmid#170322PurposeExpresses FLAG and mCherry tagged RPL22, puromycin resistanceDepositorInsertRPL22 (RPL22 Human)
UseLentiviralTagsExpressionMutationNonePromoterAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFB-U6-Chrna5-gRNA-hSyn-mCherry-WPRE-SV40pA
Plasmid#128340PurposepAAV encoding gRNA sequence for loss-of-function indelsDepositorInsertChrna5 (Chrna5 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
Human NANOS3-P2A-mCherry knock-in targeting construct
Plasmid#59721PurposeGene targeting construct for human NANOS3 locus, introducing a P2A-mCherry reporter gene. Contains an frt-PGK-Neo-PA-frt cassette that can be excised.DepositorInsertP2A mCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
Lenti-DIO-P-Ef1a-mCherry-T2A-RPL22-1xV5
Plasmid#170328PurposeDouble-floxed inverse Orientation V5 and mCherry tagged RPL22DepositorInsertRPL22 (RPL22 Human)
UseLentiviralTagsExpressionMutationNonePromoterAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
ACE Reporter (pLenti-CMV-mCherry (+43) -T2A-eGFP)
Plasmid#109428PurposeA lentiviral backbone expressing mCherry with a 43 basepair insert and eGFP off of a CMV promoter. A reporter for both Cas9 nuclease and APOBEC-mediated base-editing activity.DepositorInsertmCherry T2A GFP
UseLentiviralTagsExpressionMutationInserted 43 base pairs into mCherry to create rep…PromoterCMVAvailable sinceJuly 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR-TBP(38Q)-IDR-mCherry-Cry2-NLS
Plasmid#145269PurposeMammalian Expression of Nuclear Opto TBP(38Q) IDR with SV40 NLSDepositorInsertTBP(38Q)-IDR (TBP Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-PKAsub-mCherry-MinD-IRES-Puro
Plasmid#213118PurposeExpression of one half of a PKAsub-mCherry-MinD reporter circuit in mammalian cellsDepositorInsertPKAsub-mCherry-MinD (minD Escherichia coli)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SynI-FlpOn-CreOff-MCS-P2A-mCherry
Plasmid#176277PurposeViral vector for co-expression of a CDS of interest and mCherry in cells expressing Flp AND NOT Cre driven by a Synapsin promoter. Contains an in-frame P2A sequence and an MCS for cloning of the CDS.DepositorInsertMCS-P2A-mCherry
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterhuman Synapsin IAvailable sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
RabV CVS-N2c(deltaG)-mCherry-P2A-Cre
Plasmid#73472PurposeExpresses mCherry and Cre for monosynaptic circuit mapping and genetic manipulationDepositorInsertmCherry-P2A-Cre
UseNeurotropic virusTagsExpressionMutationPromoterAvailable sinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
PCS2-FlipGFP(Casp3 cleavage seq) T2A mCherry
Plasmid#124431PurposeExpresses FlipGFP (caspase-3 cleavage sequence) protease reporter and T2A mCherry in zebrafishDepositorInsertFlipGFP(Casp3 cleavage seq) T2A mCherry
UseZebrafish (if expression level is too high in zeb…TagsExpressionMutationPromoterAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAG385 bJun-NFAST-NLS-DEVDG-mCherry-NES
Plasmid#130816PurposeExpresses bJun-NFAST-NLS-DEVDG-mCherry-NES in mammalian cellsDepositorInsertbJun-NFAST-NLS-DEVDG-mCherry-NES
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
hEF1α-H2B-mVenus-IRES-mCherry PGK-Puromycin
Plasmid#99278PurposeDual fluorescent reporter - constitutive expression of mVenus (515/528) localized in the nucleus, mCherry (587/610) untargeted and visualized in the cytoplasm, and Puromycin resistanceDepositorInsertsH2B-mVenus-IRES-mCherry
Puro
UseLentiviralTagsH2B, IRES, PGK, mCherry, and mVenusExpressionMutationPromoterEF1 alpha-human and PGK-mouseAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-GFAP-CRY2PHR-P2A-CIBN-mCherry-VAMP2
Plasmid#178575PurposeLentiviral vector for GFAP::Opto-vTrapDepositorInsertCRY2PHR-P2A-CIBN-mCherry-VAMP2
UseLentiviralTagsExpressionMutationPromoterGFAPAvailable sinceApril 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
PylT(UAG) PylS mCherry-P2A-eGFP(150TAG)
Plasmid#174526PurposeDual-fluorescence reporter for quantifying PylT(UAG) decoding efficiencyDepositorInsertPylS
UseTagsFlagExpressionMammalianMutationPromoterEF-1αAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEx1-pEF-H2B-mCherry-T2A-rTetR-EED
Plasmid#78101PurposeExpresses rTetR-EED for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.DepositorInsertH2B-mCherry-T2A-rTetR-EED (EED Human, Synthetic)
UseSynthetic BiologyTagsrTetRExpressionMammalianMutationPromoterpEF alphaAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only