-
Plasmid#8797DepositorInsertBad (Bad Mouse)
UseTagsExpressionMammalianMutationS112APromoterAvailable sinceJuly 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pGS1022
Plasmid#59576PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Twin-Strep tag and a URA3 selection markerDepositorInsertTwin-Strep tag optimized for PCR amplification followed by URA3 selection marker
UseE. coli cloning vectorTagsTwin-Strep tagExpressionMutationPromoterNoneAvailable sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
GFP-BICD2N
Plasmid#64070PurposeN-terminal 400 amino acids of mouse BICD2 codon optimized for Sf9 expression with a N-terminal GFP tag and a TEV-cleavable His-ZZ tag for purification (GFP-BICD2N)DepositorInsertBICD2N (1-400) (Bicd2 Mouse)
UseTagsGFP and His-ZZ-TCSExpressionInsectMutationCodon optimized for Sf9 expressionPromoterAvailable sinceSept. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
tdp43-EGFP construct9
Plasmid#28202DepositorInsertTar DNA binding protein (TARDBP Human)
UseTagsEGFPExpressionMammalianMutationaa1-193 onlyPromoterAvailable sinceSept. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAC8_MF (VE5596)
Plasmid#139768PurposeTransfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette.DepositorTypeEmpty backboneUseTagsExpressionMutationPromoterPH or p10Available sinceMay 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28-His6-Ubc9-C138A
Plasmid#133911PurposeBacterial expression of His6-Ubc9-C138ADepositorInsertSUMO-conjugating enzyme UBC9 (UBE2I Human)
UseTagsThrombin-cleavable His6-tagExpressionBacterialMutationCysteine 138 to AlaninePromoterT7Available sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMuLE Lenti Dest IFP1.4
Plasmid#62177PurposeMuLE (Multiple Lentiviral Expression) Destination vector for use with pMuLE Entry vectors. Co-expresses IFP1.4 under the control of a PGK promoter. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseLentiviral; Mule gateway destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLJM60-S6K1(T389S) barcoded
Plasmid#48800Purposeexpresses mutant S6K1DepositorInsertS6K1 T389S (Rps6kb1 Rat)
UseLentiviralTagsFLAG and barcode: GGTACCExpressionMammalianMutationT389SPromoterCMVAvailable sinceDec. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-UBE2QL1-CS-Strep/HA
Plasmid#129513PurposeExpresses UBE2QL1 C88S (catalytically inactive mutant) with C-terminal Strep-HA tag in mammalian cellsDepositorInsertUBE2QL1 C88S (UBE2QL1 Human)
UseTags2xStrep/HAExpressionMammalianMutationPromoterCMVAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
3582 pcDNA3 Bad S136E
Plasmid#8800DepositorInsertBad (Bad Mouse)
UseTagsExpressionMammalianMutationS136EPromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA Flag-RARalpha (T181A,S445A,S461A)
Plasmid#35556DepositorInsertRARalpha (RARA Human)
UseTagsFlag and HisExpressionMammalianMutationT181A, S445A, S461APromoterAvailable sinceApril 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEBG-MKK4 (GST)
Plasmid#21563DepositorInsertmitogen-activated protein kinase kinase 4 (Map2k4 Mouse)
UseTagsGSTExpressionMammalianMutationPromoterAvailable sinceJuly 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-mIFITM3-K24,83,88,104A
Plasmid#58408PurposeExpresses murine IFITM3-K24,83,88,104A with an N-terminal HA tag in mammalian cells (Lysine-less nonubiquitinated mutant)DepositorInsertIFITM3 (Ifitm3 Mouse)
UseTagsHAExpressionMammalianMutationLysines 24, 83, 88, and 104 to AlaninePromoterCMV IEAvailable sinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCK351
Plasmid#129696PurposeFor insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + terminator ECK120034435DepositorInsertsPrhaBAD
yfp
rhaS
Terminator ECK120034435
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
ath5:H2B-RFP
Plasmid#105960Purposetransgenesis, appears rather late, likely due to long RFP maturation time but is brighter than most other Ath5 constructDepositorInsertH2B (H2BC21 Human)
UseTagsRFPExpressionBacterialMutationPromoterAvailable sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMuLE Lenti Dest Luc2
Plasmid#62179PurposeMuLE (Multiple Lentiviral Expression) Destination vector for use with pMuLE Entry vectors. Co-expresses firefly luciferase under the control of a PGK promoter. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseLuciferase; Mule gateway destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRK-HA-TRAF4
Plasmid#58314PurposeHA-tagged mouse TRAF4DepositorInsertTNF receptor associated factor 4 (Traf4 Mouse)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
bactin:mKate2-ras
Plasmid#105963Purposetransgenesis, ubiquitous expressionDepositorInsertRas (KRAS Human)
UseTagsmKate2ExpressionBacterialMutationPromoterAvailable sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
Plxna4-AP-His
Plasmid#71999PurposeExpresses the extracellular region of the PlexinA4 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertPlxna4 (Plxna4 Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
PMYT1_HUMAN_D0
Plasmid#79687PurposeThis plasmid encodes the kinase domain of PMYT1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertPMYT1
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only