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3XFLAG-NSarQ27G-VMAn-FKBP

Plasmid

#70229

Purpose

Expresses FLAG tagged N-terminal portion of alpha sarcin with Q27G mutation along with VMA intein for protein splicing

Depositor

Perry Howard

Article

Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:

Insert

alpha Sarcin (N-terminal portion)

Use

Tags

3XFLAG, FK506- and rapamycin-binding protein (FKB…

Expression

Mammalian

Mutation

Changed Gln 27 to Gly

Promoter

CMV

Available since

Nov. 24, 2015

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag BimL (S58D)

Plasmid

#24239

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

BimL (S58D) (BCL2L11 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Changed serine 58 to aspartic acid

Promoter

Available since

May 10, 2010

Availability

Academic Institutions and Nonprofits only
pRK-FLAG-TRAF4(K190R)

Plasmid

#58317

Purpose

FLAG-tagged mouse TRAF4 K190R mutant

Depositor

Ying Zhang

Article

Wang et al J Biol Chem. 2013 Jul 26;288(30):21784-9

Insert

TNF receptor associated factor 4 (Traf4 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

K 190 to R

Promoter

CMV

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only
pRK5 Flag RagB (CTD)

Plasmid

#112769

Purpose

express Flag-RagB (C terminal domain only)

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

Flag-RagB (C terminal domain only) (RRAGB Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5 Flag RagC (CTD)

Plasmid

#112771

Purpose

express Flag-RagC (C terminal domain only)

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

Flag-RagC (C terminal domain only) (RRAGC Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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FLAG-KIF17-short-pcDNA3

Plasmid

#47545

Purpose

Expresses FLAG epitope-tagged short form of mouse KIF17 in mammalian cells

Depositor

Richard Maurer

Insert

KIF17-short (Kif17 Mouse)

Use

Tags

FLAG epitope

Expression

Mammalian

Mutation

G152S*

Promoter

CMV

Available since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

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FLAG-PPP2R5E siRNA-resistant

Plasmid

#120861

Purpose

expresses dox-inducible PPP2R5E resistant to siRNA pool 2 used in manuscript

Depositor

Emily Foley

Article

Lee et al J Cell Sci. 2017 May 15;130(10):1760-177

Insert

PPP2R5E (PPP2R5E Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

silent mutation at aa 282-284 in PPP2RE to genera…

Promoter

Available since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pCin4-3xFLAG-MKL1 L272A

Plasmid

#19850

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Aug 11. ():.

Insert

MKL1 L272A (MRTFA Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Leucine 272 to Alanine

Promoter

Available since

March 16, 2009

Availability

Academic Institutions and Nonprofits only
pET28b-NmCas9-Flag H588A

Plasmid

#109032

Purpose

H588A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression

Depositor

Yan Zhang

Article

Rousseau et al Mol Cell. 2018 Mar 1;69(5):906-914.e4. d

Insert

CRISPR-associated endonuclease Cas9

Use

Tags

Flag Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag BimL (S44D)

Plasmid

#24235

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

BimL (S44D) (BCL2L11 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Changed serine 44 to aspartic acid

Promoter

Available since

May 10, 2010

Availability

Academic Institutions and Nonprofits only
pCMV-Flag-YAP-P85A

Plasmid

#33062

Depositor

Kunliang Guan

Article

Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

Insert

YAP (YAP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

P85A

Promoter

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3-Flag-Tipin-L195A

Plasmid

#23116

Depositor

Aziz Sancar

Article

Kemp et al J Biol Chem. 2010 Mar 15. ():.

Insert

Tipin (TIPIN Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

changed Leucine 195 to Alanine

Promoter

Available since

Feb. 8, 2010

Availability

Academic Institutions and Nonprofits only
pCin4-3xFLAG-MKL1 S449A

Plasmid

#19842

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Aug 11. ():.

Insert

MKL1 S449A (MRTFA Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Serine 449 to Alanine

Promoter

Available since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only
V25 pLP Flag - Acceptor

Plasmid

#11703

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 . 6():13.

Type

Empty backbone

Use

Cre/Lox; Acceptor vector

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

May 26, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pfast bac-flag Fbxl3

Plasmid

#51894

Purpose

Backbone plasmid is pfastBacHTa from invitrogen

Depositor

Joseph Takahashi

Article

Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

Insert

Fbxl3 (Fbxl21 Mouse)

Use

Tags

FLAG

Expression

Mutation

Promoter

polyhedrin promoter

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3 PARL-FLAG-CT S70A

Plasmid

#13620

Depositor

Luca Pellegrini

Article

Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

Insert

Presenilin-associated rhomboid-like (PARL, PARL Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine 70 to Ala

Promoter

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
flag-hKLF6 P149S (1008)

Plasmid

#49496

Purpose

expresses human Flag tagged KLF6 with P149S mutation

Depositor

Scott Friedman

Article

Lang et al Oncogene. 2012 Oct 22. doi: 10.1038/onc.

Insert

KFL6-P149S (KLF6 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

P149S

Promoter

CMV

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-FLAG/MKRN1(1-280)

Plasmid

#78752

Purpose

To overexpress MKRN1(1-280) in Mammalian Cells

Depositor

Jaewhan Song

Article

Lee et al EMBO J. 2009 Jul 22;28(14):2100-13. doi:

Insert

MKRN1 (MKRN1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

1-280

Promoter

CMV

Available since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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CMV-10-3xFLAG-LGP2-K651E

Plasmid

#58685

Purpose

Expresses LGP2 with a mutation in the CTD (K651E) in mammalian cells

Depositor

Curt Horvath

Article

Bruns et al J Biol Chem. 2013 Jan 11;288(2):938-46.

Insert

LGP2-K651E (DHX58 Human)

Use

Tags

3x FLAG

Expression

Mammalian

Mutation

mutation to CTD, K651E

Promoter

CMV

Available since

July 31, 2014

Availability

Academic Institutions and Nonprofits only
CMV-10-3xFLAG-LGP2-MIII

Plasmid

#58683

Purpose

Expresses LGP2 with a mutation to motif III in mammalian cells

Depositor

Curt Horvath

Article

Bamming et al J Biol Chem. 2009 Apr 10;284(15):9700-12

Insert

LGP2-MIII (DHX58 Human)

Use

Tags

3x FLAG

Expression

Mammalian

Mutation

threonine and serine of helicase motif III mutate…

Promoter

CMV

Available since

July 31, 2014

Availability

Academic Institutions and Nonprofits only