-
Plasmid#128160PurposePtet-controlled expression of S. pyogenes Cas9DepositorInsertSp Cas9
UseTagsExpressionBacterialMutationPromoterPtetAvailable sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUCSh5-2300
Plasmid#171788PurposeEncodes sgRNA target 2300 and Gentamicin resistance transposon.DepositorInsertSh-CAST sgRNA 2300
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119Available sinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSHS212
Plasmid#87346PurposeCysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmidDepositorInsertCas9
UseTagsHis-MBPExpressionBacterialMutationC80S/C574SPromoterT7Available sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMLS236
Plasmid#73684PurposeSapTrap 3-site Destination vector for internal GFP tagging with embedded Cbr-unc-119 selectable markerDepositorInsertGFP + Cbr-unc-119
UseCRISPR and Cre/LoxTagsGFPExpressionWormMutationPromoterAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSCBE3
Plasmid#133966PurposeExpresses BE3 CRISPR base editor and gRNA scaffold in StreptomycesDepositorArticleInsertBE3
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMLS235
Plasmid#73683PurposeSapTrap 3-site Destination vector for C-terminal GFP tagging with embedded Cbr-unc-119 selectable markerDepositorInsertGFP + Cbr-unc-119
UseCRISPR and Cre/LoxTagsExpressionWormMutationPromoterAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pARNTL.1.0-gDNA
Plasmid#112480PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ARNTLDepositorInsertARNTL (BMAL1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCF525-AcrIIA5_gb36_lenti
Plasmid#138306PurposeLenti expression of AcrIIA5DepositorInsertAcrIIA5
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSABEd
Plasmid#133968PurposeExpresses ABEd CRISPR base editor and gRNA scaffold in StreptomycesDepositorArticleInsertABEd
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSCBE2
Plasmid#133965PurposeExpresses BE2 CRISPR base editor and gRNA scaffold in StreptomycesDepositorArticleInsertBE2
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCR8/GW:U6
Plasmid#85981PurposeGolden Gate Level 0 cassette encoding the U6 promoterDepositorInsertT. pseudonana U6 promoter
UseCRISPR; Diatom (t. pseudonana)TagsExpressionMutationPromoternoneAvailable sinceJan. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKEW397_AcrIIA15
Plasmid#138285PurposeExpression of AcrIIA15 proteinDepositorInsertAcrIIA15
UseCRISPRTagsMBPExpressionBacterialMutationPromoterAvailable sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKEW394_AcrIIA14
Plasmid#138283PurposeExpression of AcrIIA14 proteinDepositorInsertAcrIIA14
UseCRISPRTagsSFGFPExpressionBacterialMutationPromoterAvailable sinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSABEn
Plasmid#133969PurposeExpresses ABEn CRISPR base editor and gRNA scaffold in StreptomycesDepositorArticleInsertABEn
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
p2CT-Cdi
Plasmid#83044PurposeExpresses Corynebacterium diphtheriae Cas9 (CdiCas9)DepositorInsertCdiCas9
UseTagsHis-MBPExpressionBacterialMutationPromoterAvailable sinceNov. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAL-rfp_T
Plasmid#74075PurposepAL374 plasmid carrying the rfp (T) sgRNA targeting the template strand of rfp, SpecRDepositorInsertsgRNA rfp (T)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterptacAvailable sinceApril 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
TU6m-gRNAscaffHygroR
Plasmid#165485PurposeS. pyogenes Cas9 guide RNA expression in tilapia cellsDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterOreochromis mossambicus U6Available sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAW209
Plasmid#113188PurposeE. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR arrayDepositorInsertsCas1
Cas2
Leader-repeat
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCF525-AcrIIA4_gb39_lenti
Plasmid#138305PurposeLenti expression of AcrIIA4DepositorInsertAcrIIA4
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEC-K-CBP_CjeCas9
Plasmid#83043PurposeExpresses Campylobacter jejuni Cas9 (Cje Cas9).DepositorInsertCjeCas9
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only