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Plasmid#154329PurposesgRNA Target Vector for CRISPRDepositorInsertcxTi10882 site targeting sgRNA (F+E) with K09B11.2 U6 promoter and 3'UTR
UseCRISPRTagsExpressionWormMutationF+E mutations in sgRNAPromoterAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
p270 pCMV-CRE-M-AR(LNCaP)
Plasmid#12496DepositorInsertCRE-M AR (AR Human)
UseCre/LoxTagsExpressionMammalianMutationThr 868 Ala in AR-LBDPromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pJW1886
Plasmid#154333PurposeMulti-cassette for SapTrapDepositorInsert30aa linker::mScarlet-I^SEC (Lox511I)^TEV::degron::3xFLAG
UseCRISPR and Cre/LoxTagsExpressionWormMutationPromoterAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOD2049-csnControl
Plasmid#100007PurposeMosSCI targeting vector expressing an ubiquitin ligase adaptor ZIF-1, serving as a control for pOD2048-csnDEG. Expression is controlled by Posm-6 (ciliated sensory neuron specific).DepositorInsertsUseTargeting vector for mos1 transposon mediated sin…TagsExpressionMutationPromoterAvailable sinceSept. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
p274 pCMV-CRE-M-AR(T)
Plasmid#12497DepositorInsertCRE-M AR (AR Human)
UseCre/LoxTagsExpressionMammalianMutationEarly termination mutationPromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pJW1788
Plasmid#154317PurposeCT Donor-cassette for SapTrapDepositorInsertlinker::TEV::BioTag::AID*::3xFLAG donor for CT slot in the SapTrap cloning system
UseCRISPRTagsExpressionWormMutationPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJW1787
Plasmid#154316PurposeNT Donor-cassette for SapTrapDepositorInsertlinker::TEV::BioTag::AID*::3xFLAG donor for NT slot in the SapTrap cloning system
UseCRISPRTagsExpressionWormMutationPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-BE3-PGK-Puro
Plasmid#110842PurposeLentiviral vector for constitutive expression of BE3 (not codon optimized)DepositorInsertBE3
UseLentiviralTagsExpressionMutationD10APromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-BE3-P2A-Puro
Plasmid#110838PurposeLentiviral vector for constitutive expression of BE3 (not codon optimized)DepositorInsertBE3
UseLentiviralTagsExpressionMutationD10APromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-BE3-FNLS
Plasmid#110836PurposeCMV expression vector for BE3-FNLS construct (not codon optimized)DepositorInsertBE3-FNLS
UseTags3X FLAGExpressionMammalianMutationNLS sequence at the N-terminusPromoterCMVAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only