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Plasmid#126688PurposeConstitutive, ubiquitous, nuclear-localized GFP expression.DepositorInsertnlsGFP
UseLentiviralTags6xHIS and NLSExpressionMutationPromoterEF1aLAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFlox c-RAD21_FKBPF36V_EYFP_Bsd
Plasmid#163871PurposeAcute Depletion of RD21-EYFPDepositorInsertRad21 (Rad21 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-EFS-GFP
Plasmid#170176PurposeExpresses GFP under the control of EF1a short promoterDepositorInsertGFP
UseAAVTagsExpressionMutationPromoterEFS promoterAvailable sinceJuly 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-gRNA-puro
Plasmid#180426PurposeExpresses puromycin resistance gene and scrambled gRNA for stable integration in mammalian cells; useful as control and template for new gRNAs by mutagenesis.DepositorInsertgRNAscr
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhuman U6Available sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
Let-7b-GFP
Plasmid#25404DepositorInsertMirlet7b (Mirlet7b Mouse)
UseLentiviralTagsExpressionMammalianMutationNonePromoterAvailable sinceJuly 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
USP19 sgRNA1
Plasmid#78585Purposedelete USP19 gene in human cellDepositorInsertUSP19 sgRNA1
UseCRISPRTagsExpressionMammalianMutationPromoterCBhAvailable sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
FRP1640_PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1
Plasmid#58440PurposeCassette to replace yeast promoters with an inducible promoter containing 2 lexA boxesDepositorInsertPTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1
UseTagsExpressionYeastMutationPromoterPTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-GFP-Metavinculin
Plasmid#107151Purposemammalian expressionDepositorInsertmetavinculin (VCL Human)
UseTags(His)10-GFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
FRP1639_PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1
Plasmid#58439PurposeCassette to replace yeast promoters with an inducible promoter containing 1 lexA boxDepositorInsertPTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1
UseTagsExpressionYeastMutationPromoterPTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
phCMV-IE1
Plasmid#118048PurposeGBPart - Cytomegalovirus enhancer and promoter for constitutive high level expression in mammalian cellsDepositorInsertCytomegalovirus enhancer and promoter
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR-PpU6P-sgRNA-L1L2
Plasmid#113735PurposeGateway entry vector containing attL1 and attL2 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.DepositorInsertPpU6P::sgRNA
UseCRISPR; Gateway entry vectorTagsExpressionMutationPromoterP. patens U6 promoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-29b(+)-mSandy2
Plasmid#177760PurposeExpresses mSandy2 using the T7 PromoterDepositorInsertmSandy2
UseTags6xHis tagExpressionBacterialMutationPromoterT7 PromoterAvailable sinceJan. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOPS0750
Plasmid#115511PurposeReporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing gusA.DepositorInsertgusA pOGG083, T-pharma pOGG003 assembled in pOGG026
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterConstitutive promoter pNeo pOGG001Available sinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
DT042A
Plasmid#159403PurposeInducible superfolder GFP (sfGFP) expression, controlled under T7 bacteriophage promoterDepositorInsertpr.T5-LacO-6xHis-sfGFP
UseTags6xHisExpressionBacterialMutationPromoterT5 promoterAvailable sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pProm1_BCD1-GFP
Plasmid#80649PurposePromoter1 with BCD1-gfpDepositorInsertGFP
UseP15a origin, constitutive promoter, kanrTagsExpressionMutationNONEPromoterconstitutive promoterAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPTK002-2-pGAP
Plasmid#84961PurposeType 2, pGAP, Glyceraldehyde-3-phosphate dehydrogenase promoterDepositorInsertGlyceraldehyde-3-phosphate dehydrogenase promoter
UseSynthetic BiologyTagsExpressionYeastMutationBsaI site removed (1805 c to g)PromoterNonAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
OPN(-213)-luc
Plasmid#31107DepositorInsertosteopontin promoter (SPP1 Human)
UseLuciferaseTagsExpressionMammalianMutationpromoter region -213 to +87PromoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFC331
Plasmid#87843PurposeAMA1 plasmid with Aspergillus optimized Cas9 and argB selection markerDepositorInsertsCas9
argB
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationPromoterAspergillus nidulans tef1 promoter and native pro…Available sinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pICH47732:PtFCP:ShBle
Plasmid#104893PurposeExpresses ShBle with FCP promoter/terminator, Golden Gate Assembly, for Phaeodactylum transformation.DepositorInsertShBle
UsePhaeodactylum; golden gate assemblyTagsPtFCP promoter and terminatorExpressionMutationPromoterAvailable sinceDec. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTR1-GPD-TRPC
Plasmid#190103PurposeExpression vector for fungal (Aspergillus flavus) genes based on pyrithiamine resistance (ptrA) selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only