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Plasmid#61933PurposeExpression of hUCH37 (M148A/F149A) in E.coliDepositorInserthUCH37 (ISF1) (UCHL5 Human)
UseTags6x HISExpressionBacterialMutationMethionine 148 and Phenylalanine 149 were mutated…PromoterT7Available sinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pETDUET1-hUCH37 (ISF1) - hNFRKB (1-117)
Plasmid#61941PurposeCo-expression of hUCH37 (ISF1) and hNFRKB (1-117) in E.coliDepositorUseTags6x HISExpressionBacterialMutationConstruct ends at residue 117, stop codon introdu…PromoterT7Available sinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
213-petDuet1-GBD-LUMP--cdc42
Plasmid#65973PurposeDual expression of GBD-LUMP and cdc42DepositorInsertsUseTagsGBD (GTPase binding domain from ACK1; residues 44…ExpressionBacterialMutationconstitutively active Cdc42 (Q61L)PromoterT7Available sinceJuly 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET11+PCNA3-CYP119(D77R/T214V)
Plasmid#66138PurposeExpresses the D77R/T214V mutant of CYP119 fused to Sulfolobus solfataricus PCNA3DepositorInsertCYP119 (cyp119 )
UseTagsSulfolobus solfataricus PCNA3ExpressionBacterialMutationD77R, T214VPromoterT7 promoterAvailable sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-22HT-MSI1(7-192)Q30D
Plasmid#60346PurposeQ30D mutant of the mouse MSI1 RBDDepositorInsertmouse MSI1 (7-192) (Msi1 Mouse)
UseTagsHis and TEV protease siteExpressionBacterialMutationQ30D; contains amino acids 7-192 of Msi1PromoterT7Available sinceDec. 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-22HT-MSI1(7-192)R53E
Plasmid#60352PurposeR53E mutant of the mouse MSI1 RBDDepositorInsertmouse MSI1 (7-192) (Msi1 Mouse)
UseTagsHis and TEV protease siteExpressionBacterialMutationR53E; contains amino acids 7-192 of Msi1PromoterT7Available sinceNov. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-22HT-MSI1(7-192)G64A
Plasmid#60351PurposeG64A mutant of the mouse MSI1 RBDDepositorInsertmouse MSI1 (7-192) (Msi1 Mouse)
UseTagsHis and TEV protease siteExpressionBacterialMutationG64A; contains amino acids 7-192 of Msi1PromoterT7Available sinceNov. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-22HT-MSI1(7-192)H83L
Plasmid#60350PurposeH83L mutant of the mouse MSI1 RBDDepositorInsertmouse MSI1 (7-192) (Msi1 Mouse)
UseTagsHis and TEV protease siteExpressionBacterialMutationH83L; contains amino acids 7-192 of Msi1PromoterT7Available sinceNov. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-22HT-MSI1(7-192)G35D
Plasmid#60347PurposeG35D mutant of the mouse MSI1 RBDDepositorInsertmouse MSI1 (7-192) (Msi1 Mouse)
UseTagsHis and TEV protease siteExpressionBacterialMutationG35D; contains amino acids 7-192 of Msi1PromoterT7Available sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-22HT-MSI1(7-192)G35E
Plasmid#60348PurposeG35E mutant of the mouse MSI1 RBDDepositorInsertmouse MSI1 (7-192) (Msi1 Mouse)
UseTagsHis and TEV protease siteExpressionBacterialMutationG35E; contains amino acids 7-192 of Msi1PromoterT7Available sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-22HT-MSI1(7-192)H83F
Plasmid#60349PurposeH83F mutant of the mouse MSI1 RBDDepositorInsertmouse MSI1 (7-192) (Msi1 Mouse)
UseTagsHis and TEV protease siteExpressionBacterialMutationH83F; contains amino acids 7-192 of Msi1PromoterT7Available sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbR-HIS-Spec(x2)-DocI
Plasmid#58713PurposeCharacteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tagDepositorInsertSpec(x2)-DocI
UseTagsHIS, HRV 3C, and ybbRExpressionBacterialMutationPromoterT7Available sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET15b/GgVcl 1-851 A50I mutation
Plasmid#46174DepositorInsertVcl 1-851 A50I (VCL Chicken)
UseTagsHisExpressionBacterialMutationaa 1-851 A50I mutation (talin binding domain mut…PromoterT7Available sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET15b/GgVcl 1-258 A50I mutation
Plasmid#46175DepositorInsertVcl 1-258 A50I (VCL Chicken)
UseTagsHisExpressionBacterialMutationaa 1-258 (VD1) A50I mutation; talin binding defic…PromoterT7Available sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28b(+)_S. cerevisiae Hbs1(162-611)
Plasmid#39337DepositorInsertElongation factor 1 alpha-like protein
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceSept. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET-21a(+)-DR RecR(K23A/R27A)
Plasmid#12619DepositorInsertDeinococcus radiodurans RecR (recR Deinococcus radiodurans)
UseTagsHisExpressionBacterialMutationchanged Lysine 23 to Alanine, and Arginine 27 to …PromoterAvailable sinceJan. 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
p141 pETF-GST RanBP10 G308R
Plasmid#11094DepositorInsertRanBP10 (Ranbp10 Mouse)
UseTagsGSTExpressionBacterialMutationG308RPromoterAvailable sinceDec. 16, 2005AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MGGG-ybbr-HIS-SdrG_B1-DK
Plasmid#117981PurposeSdrG B1 domain with N-terminal ybbr and sortase tags for covalent immobilization and C-terminal DK peptide for thethering with ClfBDepositorInsertSdrG_B1
UseTagsHIS, sortase, and ybbrExpressionBacterialMutationPromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pET28a-MGGG-ybbr-HIS-SdrG_B2-DK
Plasmid#117982PurposeSdrG B2 domain with N-terminal ybbr and sortase tags for covalent immobilization and C-terminal DK peptide for thethering with ClfBDepositorInsertSdrG_B2
UseTagsHIS, sortase, and ybbrExpressionBacterialMutationPromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pET28a-MGGG-ybbr-HIS-SdrD_B1-DK
Plasmid#117983PurposeSdrD B1 domain with N-terminal ybbr and sortase tags for covalent immobilization and C-terminal DK peptide for thethering with ClfBDepositorInsertSdrG_B2
UseTagsHIS, sortase, and ybbrExpressionBacterialMutationPromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only