-
-
pMRXIB-mRuby3-CERT(PHD)
Plasmid#221762PurposeStable expression of mRuby3-CERT(PHD) in mammalian cellsDepositorInsertCERT (CERT1 Human)
UseRetroviralTagsmRuby3ExpressionMammalianMutationamino acids 1–116PromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
LYSO-LRRK2
Plasmid#193582PurposeExpresses human full-length LRRK2 with a lysosomal targeting sequence from LAMTOR1 (1-39aa) and 3xFLAG on the N-terminusDepositorInsertLeucine-rich repeat kinase 2 (LRRK2 Human)
UseTagsLAMTOR1(1-39aa) and 3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmCherryC2-Gephyrin P1
Plasmid#68820PurposemCherry tagged Geph for mammalian expressionDepositorInsertGephyrin (Gphn Rat)
UseTagsmCherryExpressionMammalianMutationsilent mutations on internal EcoRI site. Gephyrin…PromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pICE-HA-Ku70-siR-WT
Plasmid#82329PurposePlasmid for constitutive or doxycycline-inducible expression of wild-type human Ku70 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.DepositorInsertKu70 (XRCC6 Human)
UseTagsHAExpressionMammalianMutationPromoterCMV-tetAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSynSRE-T-Luc
Plasmid#60444PurposeUses the hamster HMG-CoA synthase promoter sequence (-324/-225) and minimal HMG-CoA sythnase TATA box (-28/+39) to study SREBP-dependent transcriptional activation.DepositorInsertHMG-CoA synthase promoter (-324/-225)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterHMG-CoA synthase (-324/-225) & TATAA (-28/+39)Available sinceFeb. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pclbw-opa1(isoform 1)-myc
Plasmid#62845PurposeMammalian Expression of myc-tagged Opa1 isoform1DepositorInsertmmOPA1isoform1-myc (Opa1 Mouse)
UseRetroviralTags9xMycExpressionMammalianMutationPromotercmv-bactinAvailable sinceJan. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA GNSTM-3-RVG-10-Lamp2b-HA
Plasmid#71294PurposeEncodes (N to C): GNSTM glycosylation motif, 3 residue spacer, RVG peptide, 10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag.DepositorInsertLamp2b (LAMP2 Human)
UseTagsGNSTM glycosylation motif, HA, Lamp2 signal pepti…ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
CD69-His-bio
Plasmid#52328PurposeExpresses full-length C-type lectin domain family 2 member C ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.DepositorInsertCD69 (CD69 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/Myc-His beta4
Plasmid#16039DepositorInsertbeta4 (ITGB4 Human)
UseTagsHis and MycExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
-
NOTCH3-bio-His
Plasmid#53343PurposeExpresses full-length Neurogenic locus notch homolog protein 3 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertNOTCH3 (NOTCH3 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
-
hFLT1
Plasmid#83435Purposehuman VEGFR1 with HA and FLAG tagDepositorInserthuman VEGFR1 (FLT1 Human)
UseTagsFlag and MycExpressionMammalianMutationHA-tag was inserted 30 AA downstream of the FLT1 …PromoterCMVAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCR8/GW_TOPO_Cdk12_Nterm_FlagHa
Plasmid#127177PurposeN-terminal Flag- HA- tandem epitope tagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into Gateway entry vectorDepositorInsertN-terminally Flag- HA- Epitope Tagged Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
UseEntry vector with transgene for gateway cloningTagsFlag- HA- Tandem EpitopesExpressionMutationPromoterNoneAvailable sinceJuly 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6 asyn WT
Plasmid#107425PurposeMammalian expression of human alpha synucleinDepositorInsertalpha-synuclein (SNCA Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB-Neo-TetO-Cdk12_Untagged
Plasmid#127178PurposeUntagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into a dox-inducible piggybac destination vectorDepositorInsertMouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
UsePiggybac transposase vectorTagsNoneExpressionMammalianMutationPromoterTetO PromoterAvailable sinceJuly 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBabe EGFR Del1(T790M)
Plasmid#32072DepositorInsertEGFR (EGFR Human)
UseRetroviralTagsExpressionMammalianMutationDelete E746-A750, T790MPromoterAvailable sinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
ALB-bio-His
Plasmid#52176PurposeExpresses full-length Serum albumin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertALB (ALB Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHR-SIN-BX SpyCatcher003-hCD52
Plasmid#210565PurposeExpresses Spycatcher003 on the cell surface using human CD52 hingeDepositorInsertSpycatcher003 fused to human CD52 hinge
UseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only
Holiday Schedule: Addgene will be closing at 1:00 pm EST on November 27th and remain closed through the 29th for the Thanksgiving Holiday. Order processing and shipping may be delayed during this week. For questions about estimated ship dates, please feel free to track your order status or contact [email protected].
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.
How are we doing? Share feedback for a chance to win $50
Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more
Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more
