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Plasmid#64279Purposeencodes yeast-optimized dCas9 synthetic transcription factorDepositorInsertYeast-optimized dCas9
UseTagsSV40 NLSExpressionYeastMutationD10A & H840A relative to S. pyogenes Cas9PromoterpTPGIAvailable sinceMay 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
GluN2A (px330:2-guideCas9)
Plasmid#169428PurposeExpression of Cas9 and 2 guidesDepositorInsertspCas9
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUCSh5vio-2300
Plasmid#170987PurposeEncodes sgRNA target 2300 and Gentamicin resistance - violacein pathway transposon.DepositorInsertSh-CAST sgRNA 2300 violacein
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterplacIAvailable sinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMiniT:GFP-Gephyrin TKIT donor
Plasmid#169433PurposeDonor DNA for TKIT knockin of GFP to Gephyrin in mouseDepositorInsertEGFP
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
GluN2B (px330:2-guideCas9)
Plasmid#169430PurposeExpression of Cas9 and 2 guidesDepositorInsertspCas9
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS712
Plasmid#213041PurposeCRISPR activation based overexpression in Nakaseomyces (Candida) glabrataDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
bRA77
Plasmid#100953PurposeGAL1 driven Cas9. gRNA can be cloned into BplI sites.DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIF4
Plasmid#196069PurposeExpresses dCas9-Mxi1 with MoRP27 promoter and express gRNA with MoU6 promtoer in M. oryzaeDepositorInsertdCas9-Mxi1
UseCRISPRTagsExpressionMutationPromoterMoRP27 promoterAvailable sinceApril 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIF3
Plasmid#196068PurposeExpresses dCas9-Mxi1 with MoRP27 promoter and express gRNA with MoU3 promtoer in M. oryzaeDepositorInsertdCas9-Mxi1
UseCRISPRTagsExpressionMutationPromoterMoRP27 promoterAvailable sinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAC1813_pCR8-gSMN2-DN1
Plasmid#118646PurposeTransient transfection; Expressed SMN2-DN1 CasRx gRNA; Gateway DonorDepositorInsertgSMN2-DN1
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
Gephyrin (px330:2-guideCas9)
Plasmid#169432PurposeExpression of Cas9 and 2 guidesDepositorInsertspCas9
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAC1814_pCR8-gSMN2-DN2
Plasmid#118647PurposeTransient transfection; Expressed SMN2-DN2 CasRx gRNA; Gateway DonorDepositorInsertgSMN2-DN2
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAC1815_pCR8-gSMN2-DN3
Plasmid#118648PurposeTransient transfection; Expressed SMN2-DN3 CasRx gRNA; Gateway DonorDepositorInsertgSMN2-DN3
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
act-AsCpf1
Plasmid#73917Purposeexpression plasmid of AsCpf1DepositorInserthAsCpf1
UseTagsNLS-3xHAExpressionInsectMutationPromoteract5CAvailable sinceMarch 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-Puro.3xBsmBI
Plasmid#196709PurposeBackbone for cloning guide libraries with untranscribed barcodes and a tracrRNA of choiceDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBLlSh2
Plasmid#170981PurposeExpression of ShCAST under control of lacI Plac promoter/repressor pair.DepositorInsertslacI
ShTnsB, ShTnsC, ShTniQ, ShCas12k
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterpLlac01Available sinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKEW215_AcrIIA13
Plasmid#138281PurposeExpression of AcrIIA13 proteinDepositorInsertAcrIIA13
UseCRISPRTagsMBPExpressionBacterialMutationPromoterAvailable sinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
Construct 33 - NFKBRp_Cas9_3xNLS_p2a-puroR
Plasmid#81255PurposeLentiviral construct for building stable cell lines expressing Cas9 in the presence of TNFa via puromycin selectionDepositorInsertCas9-3xNLS
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAC1808_pmax-RBFOX1N-FRB-C
Plasmid#118641PurposeTransient transfection; Expresses RBFOX1N-FRB-C; CAGGS promoterDepositorInsertRBFOX1N-FRB-C
UseCRISPRTagsExpressionMammalianMutationPromoterCAGGSAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-Himar-dCas9
Plasmid#137079PurposeHimar-dCas9 gene with C-terminal 6x-His tag for protein purificationDepositorInsertHimar-dCas9
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only