Addgene

We narrowed to 20,429 results for: crispr

Showing: 3501 - 3520 of 20429 results

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pTPGI_dCas9

Plasmid

#64279

Purpose

encodes yeast-optimized dCas9 synthetic transcription factor

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

Yeast-optimized dCas9

Use

Tags

SV40 NLS

Expression

Yeast

Mutation

D10A & H840A relative to S. pyogenes Cas9

Promoter

pTPGI

Available since

May 5, 2015

Availability

Academic Institutions and Nonprofits only

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GluN2A (px330:2-guideCas9)

Plasmid

#169428

Purpose

Expression of Cas9 and 2 guides

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

spCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pUCSh5vio-2300

Plasmid

#170987

Purpose

Encodes sgRNA target 2300 and Gentamicin resistance - violacein pathway transposon.

Depositor

Christopher Reisch

Article

Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

Insert

Sh-CAST sgRNA 2300 violacein

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

placI

Available since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pMiniT:GFP-Gephyrin TKIT donor

Plasmid

#169433

Purpose

Donor DNA for TKIT knockin of GFP to Gephyrin in mouse

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

EGFP

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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GluN2B (px330:2-guideCas9)

Plasmid

#169430

Purpose

Expression of Cas9 and 2 guides

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

spCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

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pRS712

Plasmid

#213041

Purpose

CRISPR activation based overexpression in Nakaseomyces (Candida) glabrata

Depositor

Rebecca Shapiro

Article

Maroc et al mSphere. 2024 Feb 28;9(2):e0076123. doi:

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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bRA77

Plasmid

#100953

Purpose

GAL1 driven Cas9. gRNA can be cloned into BplI sites.

Depositor

James Haber

Article

Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pIF4

Plasmid

#196069

Purpose

Expresses dCas9-Mxi1 with MoRP27 promoter and express gRNA with MoU6 promtoer in M. oryzae

Depositor

Kabin Xie

Article

Zhang et al mSphere. 2023 Feb 21;8(1):e0059422. doi:

Insert

dCas9-Mxi1

Use

CRISPR

Tags

Expression

Mutation

Promoter

MoRP27 promoter

Available since

April 5, 2023

Availability

Academic Institutions and Nonprofits only

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pIF3

Plasmid

#196068

Purpose

Expresses dCas9-Mxi1 with MoRP27 promoter and express gRNA with MoU3 promtoer in M. oryzae

Depositor

Kabin Xie

Article

Zhang et al mSphere. 2023 Feb 21;8(1):e0059422. doi:

Insert

dCas9-Mxi1

Use

CRISPR

Tags

Expression

Mutation

Promoter

MoRP27 promoter

Available since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pAC1813_pCR8-gSMN2-DN1

Plasmid

#118646

Purpose

Transient transfection; Expressed SMN2-DN1 CasRx gRNA; Gateway Donor

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

gSMN2-DN1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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Gephyrin (px330:2-guideCas9)

Plasmid

#169432

Purpose

Expression of Cas9 and 2 guides

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

spCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

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pAC1814_pCR8-gSMN2-DN2

Plasmid

#118647

Purpose

Transient transfection; Expressed SMN2-DN2 CasRx gRNA; Gateway Donor

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

gSMN2-DN2

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAC1815_pCR8-gSMN2-DN3

Plasmid

#118648

Purpose

Transient transfection; Expressed SMN2-DN3 CasRx gRNA; Gateway Donor

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

gSMN2-DN3

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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act-AsCpf1

Plasmid

#73917

Purpose

expression plasmid of AsCpf1

Depositor

Simon Bullock

Article

Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

Insert

hAsCpf1

Use

Tags

NLS-3xHA

Expression

Insect

Mutation

Promoter

act5C

Available since

March 8, 2016

Availability

Academic Institutions and Nonprofits only

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lentiGuide-Puro.3xBsmBI

Plasmid

#196709

Purpose

Backbone for cloning guide libraries with untranscribed barcodes and a tracrRNA of choice

Depositor

Bernhard Schmierer

Article

Schmierer et al Mol Syst Biol. 2017 Oct 9;13(10):945. do

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

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pBLlSh2

Plasmid

#170981

Purpose

Expression of ShCAST under control of lacI Plac promoter/repressor pair.

Depositor

Christopher Reisch

Article

Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

Inserts

lacI
ShTnsB, ShTnsC, ShTniQ, ShCas12k

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pLlac01

Available since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pKEW215_AcrIIA13

Plasmid

#138281

Purpose

Expression of AcrIIA13 protein

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

AcrIIA13

Use

CRISPR

Tags

MBP

Expression

Bacterial

Mutation

Promoter

Available since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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Construct 33 - NFKBRp_Cas9_3xNLS_p2a-puroR

Plasmid

#81255

Purpose

Lentiviral construct for building stable cell lines expressing Cas9 in the presence of TNFa via puromycin selection

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

Cas9-3xNLS

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pAC1808_pmax-RBFOX1N-FRB-C

Plasmid

#118641

Purpose

Transient transfection; Expresses RBFOX1N-FRB-C; CAGGS promoter

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

RBFOX1N-FRB-C

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pET-Himar-dCas9

Plasmid

#137079

Purpose

Himar-dCas9 gene with C-terminal 6x-His tag for protein purification

Depositor

Harris Wang

Article

Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

Insert

Himar-dCas9

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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