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Showing: 3501 - 3520 of 3879 results

  7. MSP2283

    Plasmid
    #70702
    Purpose
    Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70702-plasmid-map-sequence-id-127769

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  14. pTorPE-GEM-GECO1

    Plasmid
    #32463
    Depositor
    Robert Campbell
    Article
    Zhao et al Science. 2011 Sep 8.
    Insert
    GEM-GECO1
    Use
    Lab constructed
    Tags
    6x His and modified TorA MNNNDLFQASRRRFLAQLG[G to…
    Expression
    Bacterial
    Mutation
    GCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…
    Promoter
    Available since
    Sept. 29, 2011
    Availability
    Academic Institutions and Nonprofits only
    32463-plasmid-map-sequence-id-37233

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  15. IBMc233

    Plasmid
    #161939
    Purpose
    Carries Golden Gate substitution insert for IBM with M86 intein. PPhlF as C-lobe promoter
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB1A3-BbsI-M86N-T-cat-PPhlF-RiboJ-B32-M86C-SapI
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PPhlF
    Available since
    March 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    161939-plasmid-map-sequence-id-327549

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  16. MSP2266

    Plasmid
    #70705
    Purpose
    Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70705-plasmid-map-sequence-id-131909

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  17. pCK300

    Plasmid
    #87766
    Purpose
    empty control plasmid, three terminators to place inserts between for insulation, ampR, pBR322 origin, lacI
    Depositor
    John Heap
    Article
    Kelly et al ACS Synth Biol. 2016 Oct 21;5(10):1136-1
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    None
    Available since
    May 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    87766-plasmid-map-sequence-id-177280

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  19. pTorPE-GEX-GECO1

    Plasmid
    #32464
    Depositor
    Robert Campbell
    Article
    Zhao et al Science. 2011 Sep 8.
    Insert
    GEX-GECO1.0
    Use
    Lab constructed
    Tags
    6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…
    Expression
    Bacterial
    Mutation
    GCaMP3 K69E/K119I/L173Q/T223S/F315L/F368L/L372Q/K…
    Promoter
    Available since
    March 30, 2012
    Availability
    Academic Institutions and Nonprofits only
    32464-plasmid-map-sequence-id-37236

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  20. Act5C-pDONR221

    Plasmid
    #124242
    Purpose
    Gateway entry clone of Drosophila Actin5C cDNA.
    Depositor
    Peter Vilmos
    Insert
    Drosophila Actin 5C (Act5C Fly)
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    April 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    124242-plasmid-map-sequence-id-240923

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Showing: 3501 - 3520 of 3879 results