-
Plasmid#46437DepositorInsertGrb10 (GRB10 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 435-548PromoterTacAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSc2
Plasmid#80437PurposeExpresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cellDepositorInsertssp Cas9 gRNA
eGFP
UseCRISPRTagsExpressionMammalianMutationPromoterCMV and U6Available sinceSept. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBullet-cg-c
Plasmid#53070Purposedestination vector with cis-golgi marker (alpha-man I 49AA) -ECFP for tagged fluorescent protein (C-ter-EYFP) of plant geneDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTBL681 CHYRON4 integration construct
Plasmid#126449PurposeTo integrate the CHYRON4 locus at AAVS1 in human cells.DepositorInsertspU6/3xLacO-CHYRON4 hgRNA
pCMV-puro
UseTagsExpressionMammalianMutationThe SpCas9 sgRNA constant region is mutated to ma…PromoterCMV and human U6/3xLacOAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET41s-GST-ATF6a-AD
Plasmid#64774Purposebacterial expression of GST-tagged ATF6 (1-150)DepositorInsertATF6a-AD (1-150) (ATF6 Human)
UseTagsGSTExpressionMammalianMutationC-term deletionPromoterAvailable sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX RASGAP(C)-SH2
Plasmid#46431DepositorInsertGap/RASGAP(C) (RASA1 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 339-452PromoterTacAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTwist-FLAG-SRSF3_Truncated_Codon.Optimized
Plasmid#172346PurposeExpression of FLAG-tagged, codon-optimized, truncated SRSF3DepositorInsertserine and arginine rich splicing factor 3 (SRSF3 Human)
UseTagsFLAGExpressionMammalianMutationcodon-optimized, truncated at amino acid 114 with…PromoterEF-1aAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV_UdgX-EE-UdgX-NG-nCas9
Plasmid#163570PurposeMammalian CG-to-GC base editingDepositorInsertUdgX-EE-UdgX-NG-nCas9
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)EE (R126E, R132E)NG (L111R, D1135V, G…PromoterAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 - HA-KLF4 FL delta K5S
Plasmid#34594DepositorInsertKLF4 (KLF4 Human)
UseTagsHA tagExpressionMammalianMutationdeletion of K5S, corresponding to bases 1781 to 2…PromoterCMVAvailable sinceJan. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGEX RASGAP(NC)-SH2
Plasmid#46433DepositorInsertGap/RASGAP(NC) (RASA1 Human)
UseTagsGSTExpressionBacterialMutationcontains amino acids 171-448PromoterTacAvailable sinceAug. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV_RBMX-eA3A-UdgX-HF-nCas9
Plasmid#163548PurposeMammalian CG-to-GC base editingDepositorInsertRBMX-eA3A-UdgX-HF-nCas9
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)HF (N497A, R661A, Q695A, Q926A)PromoterAvailable sinceDec. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX Nsp2-SH2
Plasmid#46460DepositorInsertNsp2 (BCAR3 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 144-259PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGL3-DICER-Prom Ebox double mut
Plasmid#25854DepositorInsertDICER-Promoter Ebox double mut (DICER1 Human, gene ID 23405)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBullet-vac-c
Plasmid#53076Purposedestination vector with vacuole marker (gamma-TIP) - ECFP for tagged fluorescent protein (C-ter-EYFP) of plant geneDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBullet-er-c
Plasmid#53068Purposedestination vector with WAK2 (29 aa)-ECFP- ER marker (HDEL) for tagged fluorescent protein (C-ter-EYFP) of plant geneDepositorTypeEmpty backboneUseSynthetic BiologyTagsECFP and EYFPExpressionMutationPromoterAvailable sinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX Jak3-SH2
Plasmid#46449DepositorInsertJak3 (JAK3 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 366-494PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX chimerin2-SH2
Plasmid#46416DepositorInsertchimerin2 (CHN2 Human)
UseTagsAviTag, GST, and PreScissionExpressionBacterialMutationcontains amino acids 49-155PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Mist-SH2 RK
Plasmid#46455DepositorInsertMist RK (Clnk Mouse)
UseTagsAviTag, GST, and PreScissionExpressionBacterialMutationcontains amino acids 300-435PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Grb2-SH2 RK
Plasmid#46441DepositorInsertGrb2 RK (GRB2 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 58-159PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-Flag-Med25-VWA
Plasmid#64772PurposeMammalian expression of Flag-tagged Med25-VWADepositorInsertMed25-VWA (MED25 Human)
UseTagsFlagExpressionMammalianMutationC-terminal deletionPromoterAvailable sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBullet-cyt-c
Plasmid#53066Purposedestination vector with cytosol marker (ECFP) for tagged fluorescent protein (C-ter-EYFP) of plant geneDepositorTypeEmpty backboneUseSynthetic BiologyTagsECFP and EYFPExpressionMutationPromoterAvailable sinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pWzl_neo_DEST_flag_PIK3CA
Plasmid#45308DepositorInsertPIK3CA (PIK3CA Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceMay 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGL3-DICER-Prom upstream Ebox Mut
Plasmid#25852DepositorInsertDICER-Promoter upstream Ebox Mut (DICER1 Human, gene ID 23405)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
LADL Bridge + Promoter Only Target
Plasmid#127669PurposeEncodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter as well as 2 gRNAs targeting the Zfp462 promoter expressed from their individual hU6 promotersDepositorInsertCRY2-HA-2A-mCherry and gRNAs 115 and 117
UseCRISPRTagsHAExpressionMammalianMutationPromoterEF1a and hU6Available sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL3-DICER-Prom downstream Ebox Mut
Plasmid#25853DepositorInsertDICER-Prom downstream Ebox Mut (DICER1 Human, gene ID 23405)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLAP-BUBR1-D882N
Plasmid#114062Purposetransfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purificationDepositorInsertBUBR1-D882N (BUB1B Human)
UseTagsYFP-TEV-S- Human LAPExpressionMammalianMutationC2823A and G2826A (siRNA-resistance), BUBR1-D882NPromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLAP-BUBR1-K795R
Plasmid#114060Purposetransfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purificationDepositorInsertBUBR1-K795R (BUB1B Human)
UseTagsYFP-TEV-S- Human LAPExpressionMammalianMutationC2823A and G2826A (siRNA-resistance), BUBR1-K795RPromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBullet-cg-n
Plasmid#53069Purposedestination vector with cis-golgi marker (alpha-man I 49AA) -ECFP for tagged fluorescent protein (N-ter-EYFP) of plant geneDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
LLP245 pEF1a-TALE-DNMT3a-Wt
Plasmid#100937PurposeEmpty TALE vector driven by EF1a promoter, no Puro, contains DNMT3a catalytic domainDepositorInsertTALE insertion site, DNMT3a catalytic domain (DNMT3A Human, Synthetic)
UseTALENTags3xHA and 3xTy1ExpressionMammalianMutationPromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX Grb10-PIR-SH2
Plasmid#46436DepositorInsertGrb10 (GRB10 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 355-548PromoterTacAvailable sinceAug. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Arg-SH2
Plasmid#46405DepositorInsertArg/Abl2 (ABL2 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 151-257PromoterTacAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Matk-SH2
Plasmid#46453DepositorInsertMatk (MATK Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 111-222PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX chimerin-SH2
Plasmid#46415DepositorInsertchimerin (CHN1 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 44-147PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBpuro - HA KLF4 FL delta K5S
Plasmid#34592DepositorInsertKLF4 (KLF4 Human)
UseRetroviralTagsHA tagExpressionMammalianMutationdeletion of K5S, corresponding to bases 1781 to 2…PromoterLTRAvailable sinceJan. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMiR-CIP2A(partial)
Plasmid#53705PurposeLuciferase reporter assay for microRNA binding on CIP2A coding sequenceDepositorInsertCIP2A (CIP2A Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceAug. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBullet-end-n
Plasmid#53073Purposedestination vector with ECFP- endosome marker (RabF2a) for tagged fluorescent protein (N-ter-EYFP) of plant geneDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBullet-pm-n
Plasmid#53077Purposedestination vector with PIP2a-ECFP (plasma membrane marker) for tagged fluorescent protein (N-ter-EYFP) of plant geneDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBullet-vac-n
Plasmid#53075Purposedestination vector with vacuole marker (gamma-TIP) - ECFP for tagged fluorescent protein (N-ter-EYFP) of plant geneDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBabe_puro_DEST_Flag_ARPM1
Plasmid#45259DepositorInsertARPM1 (ACTRT3 Human)
UseRetroviralTagsFLAGExpressionMammalianMutationPromoterAvailable sinceMay 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMIR-Report-Luc-KLF4-K5S-Mt344A
Plasmid#34601DepositorInsertKLF4 (KLF4 Human)
UseLuciferaseTagsExpressionMammalianMutationThe KLF4 K5S region was mutated at the miR-344 bi…PromoterCMVAvailable sinceFeb. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMIR-Report-Luc-KLF4-K5S-Mt206B
Plasmid#34600DepositorInsertKLF4 (KLF4 Human)
UseLuciferaseTagsExpressionMammalianMutationThe KLF4 K5S region was mutated at the miR-206 bi…PromoterCMVAvailable sinceFeb. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDonor-FMR1-CGG-BsmBI
Plasmid#222966Purpose0CGG with BsmBI site for inserting CGG repeatsDepositorInsertFMR1 homologous template (no CGG repeats) with cloning site (FMR1 Human)
UseCRISPRTagsExpressionMutationCGG repeats deleted from 5' UTR, BsmBI site …PromoterAvailable sinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDonor-FMR1-dCGG0
Plasmid#222965PurposeTo create 0CGG FMR1 cell lineDepositorInsertFMR1 homologous template no CGG repeats (FMR1 Human)
UseCRISPRTagsExpressionMutationCGG repeats deleted from 5' UTRPromoterAvailable sinceSept. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-CLIP-CD28(CD45TM)
Plasmid#223629PurposeLentiviral expression of human CD28 (CD45TM) with CLIP tag in mammalian cellsDepositorInsertCD28 (CD28 Human)
UseLentiviral; mammalian expressionTagsCLIPExpressionMutationCD28 transmembrane domain replaced by CD45 transm…PromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-CLIP-CD28(Y191F)
Plasmid#223628PurposeLentiviral expression of human CD28 (Y191F) with CLIP tag in mammalian cellsDepositorInsertCD28 (CD28 Human)
UseLentiviral; mammalian expressionTagsCLIPExpressionMutationY191FPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-CD28(CD45TM)
Plasmid#223626PurposeLentiviral expression of human CD28 (CD45TM) in mammalian cellsDepositorInsertCD28 (CD28 Human)
UseLentiviral; mammalian expressionTagsExpressionMutationCD28 transmembrane domain replaced by CD45 transm…PromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-Spy-CD28(CD45TM)
Plasmid#223624PurposeLentiviral expression of human CD28 (CD45TM) with SpyTag003 in mammalian cellsDepositorInsertCD28 (CD28 Human)
UseLentiviral; mammalian expressionTagsSpytag003ExpressionMutationCD28 transmembrane domain replaced by CD45 transm…PromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-Spy-CD28(Y191F)
Plasmid#223623PurposeLentiviral expression of human CD28 (Y191F) with SpyTag003 in mammalian cellsDepositorInsertCD28 (CD28 Human)
UseLentiviral; mammalian expressionTagsSpytag003ExpressionMutationY191FPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-mGFP-Eps15DN
Plasmid#223621PurposeTransient expression of human Eps15 with mGFP tag in mammalian cellsDepositorInsertEps15 (EPS15 Human)
UseTagsmGFPExpressionMammalianMutationEps15 _ 95-295aaPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-PDL1-LgBiT
Plasmid#223619PurposeLentiviral expression of human PD-L1 with LgBiT tag in mammalian cellsDepositorInsertPD-L1 (CD274 Human)
UseLentiviral; mammalian expressionTagsLgBiTExpressionMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only