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Plasmid#214808PurposeExpression of 6xhis-tagged micrococcal nuclease A (MNaseA)DepositorInsertOmpA-Micrococcal nuclease A
UseTags6xhisExpressionBacterialMutationPromoterT7 promoterAvailable sinceNov. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-T7-OmpA-MNaseB-6xHis
Plasmid#214809PurposeExpression of 6xhis-tagged micrococcal nuclease B (MNaseB)DepositorInsertOmpA-Micrococcal nuclease B
UseTags6xhisExpressionBacterialMutationPromoterT7 promoterAvailable sinceNov. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM354 pET28a-GGG-Titin[V15P]-23-K-19-C-15
Plasmid#226350PurposeExpression of proteasome substrate with tail for molecular rulerDepositorInserttitin (TTN Human)
UseTagsExpressionBacterialMutationV15PPromoterAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM355 pET28a-GGG-Titin[V15P]-23-K-24-C-10
Plasmid#226351PurposeExpression of Titin (V15P) substrate for 26S proteasomeDepositorInserttitin (TTN Human)
UseTagsExpressionBacterialMutationV15PPromoterAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM356 pET28a-GGG-Titin[V15P]-23-K-29-C-5
Plasmid#226352PurposeExpression of Titin (V15P) substrate for 26S proteasomeDepositorInserttitin (TTN Human)
UseTagsExpressionBacterialMutationV15PPromoterT7Available sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM357 pET28a-GGG-Titin[V15P]-23-K-33-C-1
Plasmid#226353PurposeExpression of titin (V15P) substrate for 26S proteasomeDepositorInserttitin (TTN Human)
UseTagsExpressionBacterialMutationV15PPromoterT7Available sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SdrG-B2-ELP-HIS-ybbR
Plasmid#169134PurposeE. coli expression of SdrG N2, N3 and B2 domains containing ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.DepositorInsertStaphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
UseTags3xELP linker, 6x Histag, and ybbr tagExpressionBacterialMutationPromoterAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-VHH-ddFLN4-HIS-ybbR
Plasmid#169137PurposeE. coli expression of VHH domain containing ybbR tag and ddFLN4 fingerprint domain. This construct was designed for AFM-SMFS measurements.DepositorInsertVHH
UseTags6x His tag, ddFLN4 tag, and ybbr tagExpressionBacterialMutationPromoterAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a- mCherry-FIVAR-HIS-ybbR
Plasmid#169138PurposeE. coli expression of mCherry domain containing ybbR tag and FIVAR fingerprint domain. This construct was designed for AFM-SMFS measurements.DepositorInsertmCherry
UseTags6x Histag, FIVAR tag, and ybbr tagExpressionBacterialMutationPromoterAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SdrG-B1-ELP-HIS-ybbR
Plasmid#169133PurposeE. coli expression of SdrG N2, N3 and B1 domains containing ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.DepositorInsertStaphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
UseTags3xELP linker, 6x Histag, and ybbr tagExpressionBacterialMutationPromoterAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-U6-sgPet-1-hSyn-mCherry-KASH
Plasmid#223227PurposeguideRNA targeting the mouse Pet-1 (Fev)DepositorInsertFev (Fev Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsmCherryExpressionMammalianMutationPromoterU6Available sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-28b Salmonella NAT_6H C-terminal
Plasmid#223436PurposeSalmonella NAT expression vector with C-terminal His6 tagDepositorInsertSalmonella NAT
UseTagsHis6ExpressionMutationPromoterAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21b-Cdc42/13C-FKBP-MBP
Plasmid#214291PurposeExpress split-Cdc42 fragment fused with FKBP and MBPDepositorInsertSplit-Cdc42 fragment fused with FKBP (CDC42 Human)
UseTagsMaltose-binding protein (MBP)ExpressionBacterialMutationCdc42-Q61LPromoterT7Available sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21b-Rac1/13C-FKBP-MBP
Plasmid#214295PurposeExpress split-Rac1 fragment fused with FKBP and MBPDepositorInsertSplit-Rac1 fragment fused with FKBP (RAC1 Human)
UseTagsMaltose-binding protein (MBP)ExpressionBacterialMutationRac1-Q61LPromoterT7Available sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21b-RhoA/13C-FKBP-MBP
Plasmid#214299PurposeExpress split-RhoA fragment fused with FKBP and MBPDepositorInsertSplit-RhoA fragment fused with FKBP (RHOA Human)
UseTagsMaltose-binding protein (MBP)ExpressionBacterialMutationRhoA-Q63LPromoterT7Available sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21b-KRas/13C-FKBP-MBP
Plasmid#214303PurposeExpress split-KRas fragment fused with FKBP and MBPDepositorInsertSplit-KRas fragment fused with FKBP (KRAS Human)
UseTagsMaltose-binding protein (MBP)ExpressionBacterialMutationKRas-Q61LPromoterT7Available sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mScarlet-TEV-Strep-His
Plasmid#210823PurposeBacterial overexpression vector for C-terminally Strep and His tagged mScarlet fluorescent proteinDepositorInsertmScarlet
UseTagsTwinStrep, 10xHisExpressionBacterialMutationPromoterT7Available sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-GAL4-PARP1-CAT-L713F
Plasmid#175949PurposeBacterial expression of a fusion of yeast GAL4 DNA binding domain and human PARP1 CAT domain containing L713F gain-of-function mutationDepositorInsertGAL4 DNA binding domain (GAL4 Budding Yeast)
UseTags6xHis tagExpressionBacterialMutationL713F, V762A for the 'PARP1-CAT-L713F' …PromoterT7Available sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21b-effector-ncRNA-rrt-His8
Plasmid#194089PurposeExpresses Ec86 operon (effector, ncRNA and reverse transcriptase) in Escherichia coliDepositorInsertEc86 effector-ncRNA-rrt
UseTagsHis8ExpressionMutationPromoterT7Available sinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-H12TEV-hEIF5B-587~1220
Plasmid#208637Purposefor E. coli expression truncated human eIF5B (ENST00000289371), aa587~1220DepositorInserthuman eIF5B (ENST00000289371) aa 587~1220 (EIF5B Human)
UseTags6xhisExpressionBacterialMutationcontain SNP rs1376443, protein sequence not chang…PromoterT7Available sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only