-
Plasmid#111820PurposeExpresses sgRNA in T. brucei cellsDepositorInsertsgRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
ACE2 g1 + Cas9
Plasmid#153011PurposeA guide RNA targeting ACE2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2DepositorUseCRISPR and LentiviralTagsTagBFP2ExpressionMutationPromoterAvailable sinceJune 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJW1219
Plasmid#61250PurposeCRISPR/Cas9 plasmid containing sgRNA(F+E); for use in C. elegansDepositorInsertsgRNA(F+E)
UseCRISPRTagsExpressionWormMutationPromoterU6 promoterAvailable sinceDec. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKm-HCBE
Plasmid#170333Purposea modularized single-plasmid cytidine base editing (C-to-T) tool for Sinorhizobium melilotiDepositorInsertRhizobium codon-optimized CBEs
UseCRISPRTagsExpressionMutationCas9n-D10APromoterAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAC1823_pCR8-RBFOX1N-dCasRx-C
Plasmid#118657PurposeGateway Donor; RBFOX1N-dCasRx-C ORF; No promoterDepositorInsertRBFOX1N-dCasRx-C
UseCRISPRTagsExpressionMammalianMutationPromoterNo promoterAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
PX851
Plasmid#62883PurposeN-term SpCas9 piece of inducible split-4 (Cas9(N)-FRB-NES split-4).DepositorInsertSpCas9 (aa 2-535)
UseCRISPRTagsFRB and NES PTK2ExpressionMammalianMutationPromoterCBhAvailable sinceMarch 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
PX852
Plasmid#62884PurposeC-term SpCas9 piece of inducible split-4 (Cas9(C)-FKBP split-4).DepositorInsertSpCas9 (aa536-1368)
UseCRISPRTagsFKBP12, NLS SV40, and NLS nucleoplasminExpressionMammalianMutationPromoterCBhAvailable sinceMarch 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
bRA66
Plasmid#100952PurposeGAL1 driven Cas9. gRNA can be cloned into BplI sites.DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKm-HGBE
Plasmid#170334Purposea modularized single-plasmid glycosylase base editing (C-to-G) tool for Sinorhizobium melilotiDepositorInsertRhizobium codon-optimized GBEs
UseCRISPRTagsExpressionMutationCas9n-D10APromoterAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
GluA3 (px330:2-guideCas9)
Plasmid#169424PurposeExpression of Cas9 and 2 guidesDepositorInsertspCas9
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
GluN1 (px330:2-guideCas9)
Plasmid#169426PurposeExpression of Cas9 and 2 guidesDepositorInsertspCas9
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
PX853
Plasmid#62885PurposeN-term SpCas9 piece of inducible split-5 (Cas9(N)-FRB-NES split-5).DepositorInsertSpCas9 (aa 2-573)
UseCRISPRTagsFRB and NES PTK2ExpressionMammalianMutationPromoterCBhAvailable sinceMarch 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
PX854
Plasmid#62886PurposeC-term SpCas9 piece of inducible split-5 (Cas9(C)-FKBP split-5).DepositorInsertSpCas9 (aa573-1368)
UseCRISPRTagsFKBP12, NLS SV40, and NLS nucleoplasminExpressionMammalianMutationPromoterCBhAvailable sinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCas1+2
Plasmid#72676PurposeExpress Cas1 and Cas2 for the CRISPR adaptation process in Escherichia coli. Has streptomycin resistance.DepositorInsertCas1, Cas2
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
beta2 (px330:2-guideCas9)
Plasmid#169435PurposeExpression of Cas9 and 2 guidesDepositorInsertspCas9
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAC1825_pCR8-dCasRx-RBM38
Plasmid#118662PurposeGateway Donor; dCasRx-RBM38 ORF; No promoterDepositorInsertdCasRx-RBM38
UseCRISPRTagsExpressionMammalianMutationPromoterNo promoterAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJAT23
Plasmid#204312PurposeFor generating targeting inversions. C-terminal split GFP, expressed in eyes.DepositorInsert3XP3-GFPc
UseCRISPRTagsExpressionMutationPromoter3XP3Available sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJW1285
Plasmid#61252PurposeCRISPR/Cas9 plasmid with sgRNA(F+E) targeting pha-1; for co-conversion in C. elegansDepositorInsertsgRNA(F+E) targeting pha-1
UseCRISPRTagsExpressionWormMutationPromoterAvailable sinceDec. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRN1120
Plasmid#101750PurposeMulti-copy (2 micron) yeast plasmid containing a functional NatMX marker cassette conferring resistance against nourseothricin. Recipient plasmid for sgRNA or crRNA expression cassettes.DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCFD4d
Plasmid#83954PurposeModified from pCFD4, with the vermillion marker and the attB site removedDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterdU6-1; dU6-3Available sinceOct. 28, 2016AvailabilityAcademic Institutions and Nonprofits only