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pcDNA-Fah_W

Plasmid

#163087

Purpose

expressing mouse wild type FAH in mammalian cells

Depositor

Joseph Takahashi

Article

Yang et al Proc Natl Acad Sci U S A. 2019 Oct 29;11

Insert

Fah_W

Use

Tags

Myc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3 PBD HK-AA (Nigg AH13)

Plasmid

#41161

Depositor

Erich Nigg

Article

Hanisch et al Mol Biol Cell. 2006 Jan;17(1):448-59. Ep

Insert

PBD HK-AA (PLK1 Human)

Use

Tags

3xMyc and PreScission Site

Expression

Mammalian

Mutation

Contains Plk1-PBD only (aa 326–603); HK-AA (H538A…

Promoter

CMV

Available since

June 7, 2013

Availability

Academic Institutions and Nonprofits only
FLAG-PAZ(aa1-480)

Plasmid

#21529

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

Ago2 (AGO2 Human)

Use

Tags

3XFLAG

Expression

Mammalian

Mutation

contain only aa1-480

Promoter

Available since

July 29, 2009

Availability

Academic Institutions and Nonprofits only
pZ_P-GAP-ACS1

Plasmid

#126712

Purpose

yeast plasmid complementing ACS1 from Komagataella phaffii

Depositor

Menghao Cai

Article

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

acs1

Use

Tags

6xHis,Myc

Expression

Yeast

Mutation

Promoter

GAP

Available since

June 19, 2019

Availability

Academic Institutions and Nonprofits only

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(517) SB KRAB-SadCas9 U6-gStop

Plasmid

#163023

Purpose

Sleeping Beauty TET-On expression of CRISPRi with gSTOP gRNA

Depositor

Tonia Rex

Article

Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

Insert

Mammalian codon-optimized SadCas9

Use

Transposon

Tags

KRAB and myc NLS

Expression

Mammalian

Mutation

Promoter

Tet-On

Available since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pMA3379

Plasmid

#46874

Purpose

A lentiviral vector for the doxycycline-inducible expression of soluble guanylate cyclase1 alpha3

Depositor

Mikhail Alexeyev, Troy Stevens

Article

Ochoa et al J Biol Chem. 2012 Jul 20;287(30):25407-1

Insert

soluble guanylate cyclase 1alpha3 (Gucy1a3 Rat)

Use

Lentiviral

Tags

Myc

Expression

Mutation

Promoter

TRE-Tight

Available since

Dec. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pCI syn iGluf

Plasmid

#106121

Purpose

Fast indicator for synaptic glutamate imaging (synapsin promoter)

Depositor

Katalin Torok

Article

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

iGluSnFR E25D variant

Use

Tags

Myc and PDGFR

Expression

Mammalian

Mutation

Changed Glu 25 to Asp

Promoter

synapsin

Available since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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(364) SB KRAB-SadCas9

Plasmid

#163022

Purpose

Sleeping Beauty TET-On expression of CRISPRi (without U6-gRNA)

Depositor

Tonia Rex

Article

Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

Insert

Mammalian codon-optimized SadCas9

Use

Transposon

Tags

KRAB and myc NLS

Expression

Mammalian

Mutation

Promoter

TET-On

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pRS406-PHO5-GFP-CERT PH domain

Plasmid

#58725

Purpose

Expresses GFP-Tagged CERT PH domain in yeast

Depositor

Tim Levine

Article

Levine et al Curr Biol. 2002 Apr 30;12(9):695-704.

Insert

collagen, type IV, alpha 3 binding protein PH domain (CERT1 Human)

Use

Tags

GFP and Myc

Expression

Yeast

Mutation

Promoter

PHO5

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only
pFRT-TODestFLAGHA_RBPMS_KDR_HindIII

Plasmid

#65662

Purpose

FLAG-HA expression vector with RBPMS carrying mutation to introduce HindIII site and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)

Depositor

Thomas Tuschl

Article

Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

Insert

RBPMS (RBPMS Human)

Use

Tags

FLAG HA

Expression

Mammalian

Mutation

introduced HindIII site (final goal to exchange F…

Promoter

CMV, 2x Tet

Available since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

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pMA3431

Plasmid

#46876

Purpose

A lentiviral vector for the doxycycline-inducible expression of soluble guanylate cyclase1 alpha3 mutant R592Q

Depositor

Mikhail Alexeyev, Troy Stevens

Article

Ochoa et al J Biol Chem. 2012 Jul 20;287(30):25407-1

Insert

soluble guanylate cyclase 1alpha 3 (Gucy1a3 Rat)

Use

Lentiviral

Tags

Myc

Expression

Mutation

R592Q

Promoter

TRE-Tight

Available since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only

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(369) pcDNA3.1(+)-HA-nLaG6-(EAAAK)4-OGA(544-706)

Plasmid

#168190

Purpose

HA-tagged GFP nanobody Lag6 (310 nM affinity) fused to a truncated OGA with residues 544-706. For use with myc-OGA(1-400) split-OGA system.

Depositor

Christina Woo

Article

Ramirez et al Curr Protoc. 2021 May;1(5):e117. doi: 10

Insert

HA-nLag6-(EAAAK)4-OGA(544-706)

Use

Tags

HA-Tag

Expression

Mammalian

Mutation

Promoter

T7/CMV

Available since

May 24, 2021

Availability

Academic Institutions and Nonprofits only

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KJ201: pMVP (R4-R3) osTir1 open

Plasmid

#121743

Purpose

pMVP R4-R3 entry plasmid, contains osTir1 (no stop codon) for 3- or 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

osTir1 (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Tags

9x myc epitope tag

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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hFlt1 V767A

Plasmid

#86038

Purpose

human VEGFR1 with V to A mutation at position 767

Depositor

Christie Thomas

Article

Raikwar et al Exp Cell Res. 2016 May 15;344(1):103-11.

Insert

human Flt1 V767A (FLT1 Human)

Use

Tags

FLAG, HA (please see depositor comments below), a…

Expression

Mammalian

Mutation

V767A

Promoter

CMV

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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L2111-FKBP-GPA-20-11S

Plasmid

#222882

Purpose

Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 11S.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FKBP-GPA-20GS-NanoLuc11S

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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L2375-FKBP-GPA-20-11S

Plasmid

#222897

Purpose

Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 11S.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FKBP-GPA-20GS-NanoLuc11S

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
L2376-FKBP-GPA-20-114

Plasmid

#222896

Purpose

Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FKBP-GPA-20GS-NanoLuc11S

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
L2374-FRB-GPA-20-11S

Plasmid

#222895

Purpose

Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 11S.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FRB-GPA-20GS-NanoLuc11S

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
L2373-FRB-GPA-20-114

Plasmid

#222894

Purpose

Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FRB-GPA-20GS-NanoLuc114

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

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L2179-FRB-GPA-6-GFPS

Plasmid

#222893

Purpose

Biosensor chain detecting rapamycin and responding with split GFP reconstitution. FRB ectodomain, human Glycophorin A (GPA) scaffold, 6 GS linker, split GFP fragment small.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FRB-GPA-6GS-GFPS

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge