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Immune checkpoint (library 2) Backbone

Plasmid

#192363

Purpose

Plasmid vector for in vivo immune checkpoint screening library (for checkpoint library)

Depositor

Lijia Ma

Article

Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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T cell activation (library 1) Backbone

Plasmid

#192364

Purpose

Plasmid vector for in vitro Jurkat activation screening (for immune library)

Depositor

Lijia Ma

Article

Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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Gly-tRNA-gRNA scaffold for library 2

Plasmid

#192365

Purpose

tRNA sequence provider (for checkpoint library)

Depositor

Lijia Ma

Article

Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

Insert

Gly tRNA-gRNA scaffold

Use

For golden gate assembly

Tags

Expression

Mutation

Promoter

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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Pro-tRNA-gRNA scaffold for library 2

Plasmid

#192366

Purpose

tRNA sequence provider (for checkpoint library)

Depositor

Lijia Ma

Article

Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

Insert

Pro tRNA-gRNA scaffold

Use

For golden gate assembly

Tags

Expression

Mutation

Promoter

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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Gly-tRNA-gRNA scaffold for library 1

Plasmid

#192368

Purpose

tRNA sequence provider (for immune library)

Depositor

Lijia Ma

Article

Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

Insert

Gly tRNA-gRNA scaffold

Use

For golden gate assembly

Tags

Expression

Mutation

Promoter

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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Pro-tRNA-gRNA scaffold for library 1

Plasmid

#192369

Purpose

tRNA sequence provider (for immune library)

Depositor

Lijia Ma

Article

Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

Insert

Pro tRNA-gRNA scaffold

Use

For golden gate assembly

Tags

Expression

Mutation

Promoter

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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KBB428

Plasmid

#185090

Purpose

NMA111-GFP with first nuclear localization signal mutated (amino acids 9 - 11, KKR -> AAA)

Depositor

Ken Belanger

Article

Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

Insert

NMA111

Use

Tags

GFP

Expression

Yeast

Mutation

First nuclear localization signal mutated (amino …

Promoter

Available since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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MSCV-Arkadia C946S H948L

Plasmid

#180373

Purpose

expressing mouse Arkadia (isoform 3) C946S H948L E3 ligase dead mutant proteins

Depositor

Dan Littman

Article

Xu et al J Exp Med. 2021 Nov 1;218(11). pii: 2126

Insert

Arkadia (Rnf111 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

C946S H948L

Promoter

LTR promoter

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pSIN-Skil-gRNA1

Plasmid

#180370

Purpose

targeting mouse Skil/SnoN gene

Depositor

Dan Littman

Article

Xu et al J Exp Med. 2021 Nov 1;218(11). pii: 2126

Insert

Skil targeting gRNA (Skil Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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pSIN-Ski-gRNA1

Plasmid

#180368

Purpose

targeting mouse Ski gene

Depositor

Dan Littman

Article

Xu et al J Exp Med. 2021 Nov 1;218(11). pii: 2126

Insert

Ski targeting gRNA (Ski Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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pODN-mNG-CfANLN

Plasmid

#183837

Purpose

Repair template for the N-terminal tagging of anillin with mNeonGreen in canine cells using CRISPR/Cas9.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

Canis familiaris ANLN homology arms with mNeonGreen-linker

Use

CRISPR; Donor template

Tags

mNeonGreen-linker

Expression

Mammalian

Mutation

Homology arms contain point mutations to remove t…

Promoter

Available since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-mR2-MYH10_sgRNA

Plasmid

#183886

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and MYH10 sgRNA for N-terminal tagging of myosin heavy chain 10 in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

MYH10 sgRNA spacer (MYH10 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot2-del458-482-dsRNAres_U

Plasmid

#147716

Purpose

Insect Expression of DmNot2-del458-482-dsRNAres

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

DmNot2-del458-482-dsRNAres (Rga Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot2-del393-429-W556E-dsRNAres_U

Plasmid

#147698

Purpose

Insect Expression of DmNot2-del393-429-W556E-dsRNAres

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

DmNot2-del393-429-W556E-dsRNAres (Rga Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pnEA-NvG-HsNot2_344-540_T

Plasmid

#147618

Purpose

Bacterial Expression of HsNot2_344-540

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot2_344-540 (CNOT2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot2-F502A_S

Plasmid

#147501

Purpose

Mammalian Expression of HsNot2-F502A

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot2-F502A (CNOT2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmEDC3_1-101-V5His6_D

Plasmid

#146121

Purpose

Insect Expression of DmEDC3_1-101

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmEDC3_1-101 (Edc3 Fly)

Use

Tags

Expression

Insect

Mutation

one silent mutation A456G and three mutations N16…

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmEDC3_1-338-V5His6_D

Plasmid

#146122

Purpose

Insect Expression of DmEDC3_1-338

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmEDC3_1-338 (Edc3 Fly)

Use

Tags

Expression

Insect

Mutation

one silent mutation A456G and three mutations N16…

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmEDC3_102-680_D

Plasmid

#146124

Purpose

Insect Expression of DmEDC3_102-680

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmEDC3_102-680 (Edc3 Fly)

Use

Tags

Expression

Insect

Mutation

one silent mutation A456G and three mutations N16…

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmEDC3_331-680_D

Plasmid

#146125

Purpose

Insect Expression of DmEDC3_331-680

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmEDC3_331-680 (Edc3 Fly)

Use

Tags

Expression

Insect

Mutation

one silent mutation A456G and three mutations N16…

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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