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Plasmid#179461PurposeGenetically encoded voltage indicator (GEVI) JEDI-2P under the promoter hsp70 for Drosophila (insect) expressionDepositorInsertJEDI-2P
UseTagsExpressionInsectMutationPromoterhsp70Available sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
PBRPyCAG-LoxP-ERAS-STOPcassette-Loxp-DsRed-IRES-PURO
Plasmid#52419PurposeConstitutive mammalian expression of ERAS. Upon CRE mediated deletion of ERAS insert, dsRED expression markers is truned on due to concomittant removal of stop cassette.DepositorInsertERAS (ERAS Human)
UseTagsExpressionMutationPromoterCAGGSAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-FRB-MCU-Flag-TetOFF
Plasmid#185659PurposeTet/dox-repressible expression of N-terminus FRB tagged human mitochondrial calcium uniporter with C-terminus Flag epitopeDepositorInsertMitochondrial calcium uniporter (MCU Human)
UseLentiviralTagsFRB and FlagExpressionMammalianMutationPromoterpTight TREAvailable sinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pINTO-N3::hTET3 V908L
Plasmid#136370PurposeExpresses epitope-tagged human TET3 V908L variant in mammalian cellsDepositorInsertTET3 (TET3 Human)
UseTags2xStrep-tagII, FLAG, and HAExpressionMammalianMutationV908LPromoterAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pINTO-N3::hTET3 V1089M
Plasmid#136369PurposeExpresses epitope-tagged human TET3 V1089M variant in mammalian cellsDepositorInsertTET3 (TET3 Human)
UseTags2x Strep-tagII, FLAG, and HAExpressionMammalianMutationV1089MPromoterAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pINTO-N3::hTET3 A1076T
Plasmid#136368PurposeExpresses epitope-tagged human TET3 A1076C variant in mammalian cellsDepositorInsertTET3 (TET3 Human)
UseTags2xStrepTagII, FLAG, and HAExpressionMammalianMutationA1076TPromoterAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pINTO-N3::hTET3 R752C
Plasmid#136367PurposeExpresses epitope-tagged human TET3 R752C variant in mammalian cellsDepositorInsertTET3 (TET3 Human)
UseTags2xStrepTagII, FLAG, and HAExpressionMammalianMutationR752CPromoterAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pINTO-N3::hTET3 F1072C
Plasmid#136366PurposeExpresses epitope-tagged human TET3 F1072C variant in mammalian cellsDepositorInsertTET3 (TET3 Human)
UseTags2xStrepTagII, FLAG, and HAExpressionMammalianMutationF1072CPromoterAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2 blast
Plasmid#98293PurposeVariant of lentiCRISPRv2 that confers blasticidin S resistanceDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterEF-1aAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_ACh3.0
Plasmid#121922Purposeexpress the genetically-encoded fluorescent acethycholine(ACh) sensor GRAB-ACh3.0DepositorHas ServiceAAV9InsertGPCR activation based ACh sensor GRAB-ACh3.0
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceJan. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
Td-CGBE
Plasmid#196599PurposeMammalian expression of Td-CGBEDepositorInsertbpNLS-TadA-8e (N46L)-32aa linker-nSpCas9-bpNLS
UseTags6*HisExpressionMammalianMutationTadA-8e (N46L); S. pyogenes Cas9 (D10A)PromoterAvailable sinceMarch 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
lenti-SpCas9 blast
Plasmid#104997PurposeThis lentiviral construct delivers hSpCas9 and blasticidin S resistance.DepositorInsertKpnI-XhoI-BsrGI-NheI
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-iCreV
Plasmid#140135Purposecan be used to generate AAV virus that will express light-inducible site-specific iCreV recombinaseDepositorHas ServiceAAV PHP.eB and AAV1InsertiCreV
UseAAVTagsExpressionMutationPromoterEF1aAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-iDreV
Plasmid#140136Purposecan be used to generate AAV virus that will expresslight-inducible site-specific iDreV recombinaseDepositorHas ServiceAAV PHP.eB and AAV1InsertiDreV
UseAAVTagsExpressionMutationPromoterEF1aAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-iFlpV
Plasmid#140137Purposecan be used to generate AAV virus that will express light-inducible site-specific iFlpV recombinaseDepositorHas ServiceAAV PHP.eB and AAV1InsertiFlpV
UseAAVTagsExpressionMutationPromoterEF1aAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-GRAB_ACh3.0
Plasmid#121923Purposeexpress the genetically-encoded fluorescent acethycholine(ACh) sensor GRAB-ACh3.0 in a cre-dependent mannerDepositorInsertGPCR activation based ACh sensor GRAB-ACh3.0
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceJan. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pLX_TRC311/NLS-Cas13d-P2A-Blast
Plasmid#212196PurposeCasRx (NLS-RfxCas13d-NLS) with 2A-Blasticidin for RNA targeting. CasRx-2A-Blasticidin is driven by EF1a promoter. EGFP is driven by SV40DepositorInsertCasRx (NLS-RfxCas13d-NLS)-HA with 2A-Blasticidin
UseCRISPR and LentiviralTagsHA, NLS, and blasticidin S deaminaseExpressionMammalianMutationPromoterEF-1α promoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
plx304-PHB-V5
Plasmid#122230PurposeExpresses human PHB attached to a V5 tagDepositorInsertProhibitin (PHB1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceAug. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-CCreV
Plasmid#140132Purposecan be used to generate AAV virus that will express fusion protein of split Cre (C-terminal) and fungal photoreceptor Vivid (VVD) monomerDepositorInsertCCreV
UseAAVTagsExpressionMutationPromoterEF1aAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only