Addgene

We narrowed to 3,798 results for: SPL

Showing: 3481 - 3500 of 3798 results

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pcDNA3_ERBB2-V5

Plasmid

#214612

Purpose

ERBB2 receptor for ERBB2/ERBB3 heterodimer split TEV recuitment assay

Depositor

Michael Wehr

Article

Popovic et al STAR Protoc. 2024 Apr 16;5(2):102987. do

Insert

ERBB2 (ERBB2 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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Myc-CYLD-C601A

Plasmid

#60027

Purpose

Myc-tagged CYLD catalytic inactive (C601A) expression

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

Insert

Cylindromatosis (CYLD Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Cysteine 601 changed to Alanine

Promoter

CMV

Available since

Nov. 25, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-pegSERPINA1-U6-Nicking-PE2-N

Plasmid

#164907

Purpose

Express a pegRNA targeting SERPINA1, and a nicking sgRNA and the N-terminal split-intein fragment of PE2

Depositor

Wen Xue

Article

Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

Insert

pegSERPINA1, Nicking sgRNA, PE2-N

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only

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G1397 DddAtox-C–SaKKH-Cas9(D10A)

Plasmid

#157840

Purpose

expresses split DddAtox-Cas9 construct in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

G1397 DddAtox-C–SaKKH-Cas9(D10A)–UGI–SV40 NLS

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)-Caprola_09

Plasmid

#194670

Purpose

Protein production of the split HaloTag based calcium recorder Caprola_09 in bacteria

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_09

Use

Tags

His-tag and Strep-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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CMV_Npu-BE3.9max C-terminal

Plasmid

#137172

Purpose

Expresses the C-terminal split-intein fragment of BE3.9max from the CMV promoter

Depositor

David Liu

Article

Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

Insert

Npu-BE3.9max_C-terminal

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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CMV_Npu-BE3.9max N-terminal

Plasmid

#137171

Purpose

Expresses the N-terminal split-intein fragment of BE3.9max from the CMV promoter

Depositor

David Liu

Article

Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

Insert

Npu-BE3.9max_N-terminal

Use

Tags

Expression

Mammalian

Mutation

Cas9 D10A

Promoter

CMV

Available since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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CMV_Npu-ABEmax C-terminal

Plasmid

#137174

Purpose

Expresses the C-terminal split-intein fragment of ABEmax from the CMV promoter

Depositor

David Liu

Article

Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

Insert

Npu-ABEmax_C-terminal

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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CMV_Npu-ABEmax N-terminal

Plasmid

#137173

Purpose

Expresses the N-terminal split-intein fragment of ABEmax from the CMV promoter

Depositor

David Liu

Article

Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

Insert

Npu-ABEmax_N-terminal

Use

Tags

Expression

Mammalian

Mutation

Cas9 D10A

Promoter

CMV

Available since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pBabe puro RalA Q75L

Plasmid

#19719

Depositor

Channing Der

Article

Lim et al Curr Biol. 2006 Dec 19. 16(24):2385-94.

Insert

V-ral simian leukemia viral oncogene homolog A (RALA Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

GTPase deficient & constitutively active Gl…

Promoter

Available since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only

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pCMV iGluu

Plasmid

#105832

Purpose

Fast indicator for extracellular glutamate imaging

Depositor

Katalin Torok

Article

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

iGluSnFR S72T variant

Use

Tags

Myc and PDGFR

Expression

Mammalian

Mutation

Changed Ser 72 to Thr

Promoter

CMV

Available since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-SspB-EGFP-VP16-P2A-FLAG-Gal4DBD-CapN-SsrA-CapC-HA

Plasmid

#213536

Purpose

Expresses the split transcription factors in mouse liver

Depositor

Wenjing Wang

Article

Shen et al Nat Methods. 2023 Jan;20(1):112-122. doi

Insert

SspB-EGFP-VP16-P2A-FLAG-Gal4DBD-CapN-SsrA-CapC-HA-HA

Use

AAV

Tags

EGFP and HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBCMV-HaloTag-VPg(FCV)

Plasmid

#167311

Purpose

Template pDNA to prepare a split CaVT component mRNA

Depositor

Hirohide Saito

Article

Nakanishi et al Cell Chem Biol. 2021 Jan 20. pii: S2451-

Insert

HaloTag-VPg(FCV)

Use

Synthetic Biology

Tags

DYK-tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pSCT-GAL93-SFPQ

Plasmid

#46320

Depositor

Steven Brown

Article

Kowalska et al Mol Cell Biol. 2012 Nov;32(22):4585-94.

Insert

SFPQ (SFPQ Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA5 FRT TO myc hSeparase

Plasmid

#59820

Purpose

Allows the integration of myc hSeparase in the genome and Tet-inducible expression.

Depositor

Stephen Taylor

Article

Holland et al J Cell Sci. 2006 Aug 15;119(Pt 16):3325-

Insert

Separase (ESPL1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pBabe puro RalA S31N

Plasmid

#19718

Depositor

Channing Der

Article

Lim et al Curr Biol. 2006 Dec 19. 16(24):2385-94.

Insert

V-ral simian leukemia viral oncogene homolog A (RALA Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

S31N mutant for dominant neg function. NOTE: lo…

Promoter

Available since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
pCDF11-SFPQ(276-598)

Plasmid

#135437

Purpose

Expresses SFPQ 276-598 with TEV-cleavable hexahistidine tag

Depositor

Charles Bond

Article

Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

Insert

Splicing Factor Proline And Glutamine Rich (SFPQ Human)

Use

Tags

hexahistidine

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pTRE-Tight-FLAG-CYLD-WT

Plasmid

#60030

Purpose

WT CYLD under control of Tet promoter with N-terminal FLAG tag

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

Insert

Cylindromatosis (CYLD Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Nov. 25, 2014

Availability

Academic Institutions and Nonprofits only
CMV_Npu-saBE3.9max C-terminal

Plasmid

#137180

Purpose

Expresses the C-terminal split-intein fragment of S. aureus BE3.9max from the CMV promoter

Depositor

David Liu

Article

Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

Insert

Npu-saBE3.9max_C-terminal

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)-Caprola_14

Plasmid

#194675

Purpose

Protein production of the split HaloTag based calcium recorder Caprola_14 in bacteria

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_14

Use

Tags

His-tag and Strep-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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