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Plasmid#73276PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-3 (Chr XI: 93378..94567)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only
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pAAV-Ef1a-FAS-TdTomato-WPRE-pA
Plasmid#37092PurposeExpresses tdTomato in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites)DepositorInsertTdTomato
UseAAV and Cre/Lox; Cre-offTagsExpressionMutationPromoterEF1aAvailable sinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-TIE-V5/HIS
Plasmid#205612PurposeExpression of human TIE receptor tyrosine kinase in mammalian cellsDepositorInsertTIE receptor tyrosine kinase (TIE1 Human)
UseTagsV5-HisExpressionMammalianMutationPromoterCMVAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTW040
Plasmid#64769PurposeFor integration of Cre recombinase at the yeast HIS3 locus orthe CYC1 terminatorDepositorInsertCre-EBD78
UseTagsfused to the human Estrogen Binding Domain (EBD),…ExpressionYeastMutationPromoterTDH3Available sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
MK14_HUMAN_D0
Plasmid#79680PurposeThis plasmid encodes the kinase domain of MK14. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertMK14
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
mCherry-NES-SspB-C3Gcat-P2A-iLIDcaax
Plasmid#173874PurposeMammalian expression of mVenus-NES-SspB-C3Gcat-P2A-iLIDcaax (opto-Rap)DepositorInsertmCherry-NES-SspB-C3Gcat-P2A-iLIDcaax
UseTagsC-terminal CAAX-box and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
tdp43-EGFP construct5
Plasmid#28198DepositorInsertTar DNA binding protein (TARDBP Human)
UseTagsEGFPExpressionMammalianMutationaa257-414 onlyPromoterAvailable sinceSept. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
tdp43-EGFP construct6
Plasmid#28199DepositorInsertTar DNA binding protein (TARDBP Human)
UseTagsEGFPExpressionMammalianMutationaa1-314 onlyPromoterAvailable sinceSept. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pExpreS2-1-C3-10H-SIII
Plasmid#175444PurposeHiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal 10His-TwinStrep tagged proteins with ExpreS2 PlatformDepositorTypeEmpty backboneUseTags3C-10His-TwinStrep and BiP Secretion PeptideExpressionInsectMutationPromoterFused Actin-HSP70Available sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLXSN IkB alpha M
Plasmid#12330DepositorInsertIkB alpha M (Nfkbia Mouse)
UseRetroviralTagsExpressionMammalianMutationDominant-negative mutant. Many signal transductio…PromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
tdp43-EGFP construct7
Plasmid#28200DepositorInsertTar DNA binding protein (TARDBP Human)
UseTagsEGFPExpressionMammalianMutationaa1-274 onlyPromoterAvailable sinceSept. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCK354
Plasmid#129698PurposeFor insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + terminator ECK120010799DepositorInsertsPrhaBAD
yfp
rhaS
Terminator ECK120010799
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMuLE Lenti Dest iRFP
Plasmid#62176PurposeMuLE (Multiple Lentiviral Expression) Destination vector for use with pMuLE Entry vectors. Co-expresses iRFP under the control of a PGK promoter. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseLentiviral; Mule gateway destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBR322-MCK1.35lux
Plasmid#53368Purposeexpresses luciferase under 1.35 kb muscle creatine kinase promoter/enhancerDepositorInsertmuscle creatine kinase promoter/enhancer 1.35kb
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
tdp43-EGFP construct8
Plasmid#28201DepositorInsertTar DNA binding protein (TARDBP Human)
UseTagsEGFPExpressionMammalianMutationaa275-313 deletedPromoterAvailable sinceSept. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
mVenus-NES-SspB-LARG-DH-P2A-iLIDcaax
Plasmid#173863PurposeMammalian expression of mVenus-NES-SspB-LARG-DH-P2A-iLIDcaax (opto-RhoA)DepositorInsertmVenus-NES-SspB-LARG-DH-P2A-iLIDcaax
UseTagsC-terminal CAAX-box and mVenusExpressionMammalianMutationPromoterCMVAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBSFI-myr-akt-K179M
Plasmid#49188PurposecDNA of Akt1 with myristyolation signal and point mutation from K to M at position 179 in cloning vectorDepositorInsertAkt1 (Akt1 Mouse)
UseAdaptor plasmidTagsHA tag and myristolation signalExpressionMutationK179MPromoterT7Available sinceDec. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
TUBG1
Plasmid#87954Purposeexpresses human gamma-tubulin with a GFP-tag on the N-terminal of the proteinDepositorInsertgamma-tubulin 1 (TUBG1 Human)
UseTagsGFPExpressionMammalianMutationwhole protein, The C-terminal is a sh-gamma-tubul…PromoterAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5-LAP-MPS1-KD
Plasmid#114045PurposeExpression of exogenous LAP tagged MPS1 Kinase-deadDepositorInsertMPS1-KD (TTK Human)
UseTagsExpressionMammalianMutationD664A kinase deadPromoterAvailable sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCfB3052(gRNA X-4, XI-3, XII-5)
Plasmid#73294PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-4, XI-3, and XII-5DepositorInsertguiding RNA
UseCRISPR; GrnaTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only