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pNK1508

Plasmid

#219750

Purpose

pGAP-like vector encoding Aspergillus nidulans 4'-phosphopantetheinyl transferase NpgA under control of GAP promoter, for yeast expression

Depositor

Alexander Mishin

Article

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

pGAP - npgA - tAOX

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pNK7184

Plasmid

#219756

Purpose

Vector for expression PzPKS2 in mammalian cells (HEK293NT)

Depositor

Karen Sarkisyan

Article

Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

Insert

PzPKS2

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pNK7189

Plasmid

#219759

Purpose

Vector for expression At4CL2 in mammalian cells (HEK293NT)

Depositor

Karen Sarkisyan

Article

Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

Insert

At4CL2

Use

Tags

Expression

Mammalian

Mutation

Promoter

pEF1

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pNK7191

Plasmid

#219764

Purpose

MoClo-compatible Level M for expression PpASCL in plants (N.benthamiana, BY2 cells)

Depositor

Karen Sarkisyan

Article

Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

Insert

PpASCL

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pNK7181

Plasmid

#219766

Purpose

Vector for expression HmS in yeast (P.pastoris)

Depositor

Karen Sarkisyan

Article

Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

Insert

HmS

Use

Tags

Expression

Yeast

Mutation

Promoter

pGAP

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pNK7182

Plasmid

#219767

Purpose

Vector for expression PpASCL in yeast (P.pastoris)

Depositor

Karen Sarkisyan

Article

Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

Insert

PpASCL

Use

Tags

Expression

Yeast

Mutation

Promoter

pGAP

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pNK7188

Plasmid

#219768

Purpose

Vector for expression Pv4CL1 in yeast (P.pastoris)

Depositor

Karen Sarkisyan

Article

Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

Insert

Pv4CL1

Use

Tags

Expression

Yeast

Mutation

Promoter

pGAP

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-AtSLAC1 WT

Plasmid

#212946

Purpose

Vector used for expression of AtSLAC1 WT in mammalian cell

Depositor

Sangho Lee

Article

Lee et al Nat Commun. 2023 Nov 14;14(1):7345. doi:

Insert

Slow anion channel-associated 1 (OZS1 Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-AtSLAC1 6D

Plasmid

#212947

Purpose

Vector used for expression of AtSLAC1 6D in mammalian cell

Depositor

Sangho Lee

Article

Lee et al Nat Commun. 2023 Nov 14;14(1):7345. doi:

Insert

Slow anion channel-associated 1 (OZS1 Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

T62D/S65D/S107D/S124D/S146D/S152D

Promoter

CMV

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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EGFP-NIP45 delta-SLD1

Plasmid

#210264

Purpose

Inducible expression of N-terminally EGFP-tagged NIP45 delta-SLD1. Use with Flp-In T-Rex system.

Depositor

Niels Mailand

Article

Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

Insert

NIP45 delta-SLD1 (delta aa 260-335) (NFATC2IP Synthetic)

Use

Tags

EGFP

Expression

Mammalian

Mutation

delta-SLD1

Promoter

CMV/TetO2

Available since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-NIP45 D394R

Plasmid

#210265

Purpose

Inducible expression of N-terminally EGFP-tagged NIP45 D394R resistant to siRNA. Use with Flp-In T-Rex system.

Depositor

Niels Mailand

Article

Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

Insert

NIP45 D394R siRES (NFATC2IP Synthetic)

Use

Tags

EGFP

Expression

Mammalian

Mutation

D394R, siRES

Promoter

CMV/TetO2

Available since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-NIP45 delta-N1

Plasmid

#210268

Purpose

Inducible expression of N-terminally EGFP-tagged NIP45 delta-N1 resistant to siRNA. Use with Flp-In T-Rex system.

Depositor

Niels Mailand

Article

Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

Insert

NIP45 delta-N1 (aa 208-419) siRES (NFATC2IP Synthetic)

Use

Tags

EGFP

Expression

Mammalian

Mutation

delta-N1, siRES

Promoter

CMV/TetO2

Available since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-NIP45 delta-N2

Plasmid

#210269

Purpose

Inducible expression of N-terminally EGFP-tagged NIP45 delta-N2 resistant to siRNA. Use with Flp-In T-Rex system.

Depositor

Niels Mailand

Article

Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

Insert

NIP45 delta-N2 (aa 261-419) siRES (NFATC2IP Synthetic)

Use

Tags

EGFP

Expression

Mammalian

Mutation

delta-N2, siRES

Promoter

CMV/TetO2

Available since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-NIP45 D394R

Plasmid

#210267

Purpose

Inducible expression of N-terminally mCherry-tagged NIP45 D394R resistant to siRNA. Use with Flp-In T-Rex system.

Depositor

Niels Mailand

Article

Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

Insert

NIP45 D394R siRES (NFATC2IP Synthetic)

Use

Tags

mCherry

Expression

Mammalian

Mutation

D394R, siRES

Promoter

CMV/TetO2

Available since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-NIP45 delta-SLD2

Plasmid

#210263

Purpose

Inducible expression of N-terminally EGFP-tagged NIP45 delta-SLD2. Use with Flp-In T-Rex system.

Depositor

Niels Mailand

Article

Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

Insert

NIP45 delta-SLD2 (aa 1-343) (NFATC2IP Synthetic)

Use

Tags

EGFP

Expression

Mammalian

Mutation

delta-SLD2

Promoter

CMV/TetO2

Available since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pQE-80L 2xHis-LRRK2 Armadillo-K17A

Plasmid

#186019

Purpose

2xHis-LRRK2 Armadillo-K17A

Depositor

Suzanne Pfeffer

Article

Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

Insert

2xHis-LRRK2 Armadillo-K17A (LRRK2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pQE-80L 2xHis-LRRK2 Armadillo- K18A

Plasmid

#186020

Purpose

2xHis-LRRK2 Armadillo- K18A

Depositor

Suzanne Pfeffer

Article

Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

Insert

2xHis-LRRK2 Armadillo- K18A (LRRK2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV Flag-LRRK2 K17A/K18A

Plasmid

#186012

Purpose

Flag-LRRK2 K17A/K18A

Depositor

Suzanne Pfeffer

Article

Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

Insert

Flag-LRRK2 K17A/K18A (LRRK2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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H11-mScarletLAMA4

Plasmid

#172439

Purpose

Hipp11 targeting vector, expresses mScarlet-mLAMA4

Depositor

Tyler Jacks

Article

Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

Insert

mScarlet-mLAMA4

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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hTln1-R1R2-17b

Plasmid

#191427

Purpose

Expresses a variant of the human TLN1 R1R2 domains containing a 17AA insert into R2 (exon 17b) in bacteria

Depositor

Ben Goult

Article

Gallego-Paez et al J Cell Biol. 2023 May 1;222(5):e20220901

Insert

Human Talin1 R1R2 domains (TLN1 Human)

Use

Tags

His-tag, TEV cleavage site

Expression

Bacterial

Mutation

contains 17AA insertion from inclusion of exon 17b

Promoter

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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