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pCMV10/3xflag-Clock L74E

Plasmid

#47338

Purpose

Mutation used for co-IPs

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

Clock (Clock Mouse)

Use

Tags

3x Flag

Expression

Mammalian

Mutation

L74E

Promoter

CMV

Available since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
HOXA9 (murine) FLAG pET

Plasmid

#8494

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

Insert

HOXA9 (Hoxa9 Mouse)

Use

Tags

FLAG and His

Expression

Bacterial

Mutation

FLAG epitope tag replaces T7 tag in the pET back…

Promoter

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3-Flag-Smad4 L43S

Plasmid

#80889

Purpose

mammalian expression of Smad4 L43S

Depositor

Aristidis Moustakas

Article

Moren et al Oncogene. 2000 Sep 7;19(38):4396-404.

Insert

Smad4 (SMAD4 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

pancreatic cancer mutation L43S

Promoter

CMV

Available since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only
pCMV5-FLAG-MKK6-K82M

Plasmid

#21583

Depositor

John Kyriakis

Article

Yuasa et al J Biol Chem. 1998 Aug 28. 273(35):22681-

Insert

MKK6 (MAP2K6 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

lysine 82 to methionine

Promoter

Available since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA5-FRT-TO-N-Flag-8His

Plasmid

#157677

Purpose

N-terminal tagging vector

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Flag-8His

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pFlag-CMV2-Brd4 S1351R

Plasmid

#21941

Depositor

Eric Verdin

Article

Bisgrove et al Proc Natl Acad Sci U S A. 2007 Aug 21. 1

Insert

BRD4 (BRD4 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

aa1209-1362 only, S1351R

Promoter

Available since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only
pFLAG-GATA2-D377-415

Plasmid

#1421

Depositor

Gokhan Hotamisligil

Article

Tong et al Mol Cell Biol 2005 Jan;25(2):706-15.

Insert

GATA-2 (GATA2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

deleted amino acids 377 to 415

Promoter

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pFLAG-GATA2-D415-475

Plasmid

#1422

Depositor

Gokhan Hotamisligil

Article

Tong et al Mol Cell Biol 2005 Jan;25(2):706-15.

Insert

GATA-2 (GATA2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

deleted amino acids 414 to 475

Promoter

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pCDNA3 humanN-Flag SadBS

Plasmid

#66881

Purpose

Mammalian expression vector for expressing N-terminally Flag-tagged short isoform of SadB

Depositor

Maria Alvarado-Kristensson

Article

Eklund et al J Biol Chem. 2014 Aug 1;289(31):21360-73

Insert

SadB (BRSK1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 25, 2015

Availability

Academic Institutions and Nonprofits only
pCDH-puro/Flag PD-L1 N200Q

Plasmid

#121451

Purpose

expression of human PD-L1 mutant in mammalian cells

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Insert

CD274 (CD274 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

N200Q

Promoter

CMV

Available since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
p6875 NTap-FLAG-HA-BPV1 E6

Plasmid

#37047

Depositor

Peter Howley

Article

Tan et al Proc Natl Acad Sci U S A. 2012 Apr 30.

Insert

BPV1 E6

Use

Tags

Flag and HA

Expression

Mammalian

Mutation

Promoter

MSCV

Available since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT-Flag-EloB

Plasmid

#31983

Purpose

Mammalian expression of Flag-tagged Elongin B

Depositor

Joan Conaway

Article

Mahrour et al J Biol Chem. 2008 Mar 21;283(12):8005-13

Insert

Elongin B (Tceb2 Rat)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

July 3, 2013

Availability

Academic Institutions and Nonprofits only
pLPC-NFLAG TRF1 deltaA

Plasmid

#16063

Depositor

Titia de Lange

Article

Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

Insert

hTRF1 (TERF1 Human)

Use

Retroviral

Tags

Flag

Expression

Mutation

Deleted acidic domain (aa1-66)

Promoter

Available since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only
pCEP4-HA-Flag-natT1R2 ECD

Plasmid

#113954

Purpose

mammalian expression plasmid for FLAG-tagged human T1R2 extracellular domain with signal peptide of influenza hemagglutinin

Depositor

Erik Procko

Article

Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

Insert

T1R2 (TAS1R2 Human)

Use

Tags

FLAG and Signal/leader sequence from influenza he…

Expression

Mammalian

Mutation

residues 22-568

Promoter

cmv

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 PARL-FLAG-CT H335G

Plasmid

#13749

Depositor

Luca Pellegrini

Article

Cipolat et al Cell. 2006 Jul 14. 126(1):163-75.

Insert

Presenilin-associated rhomboid-like (PARL, PARL Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Changed catalytic His 335 to Gly

Promoter

Available since

Feb. 1, 2007

Availability

Academic Institutions and Nonprofits only
pLPC FLAG hRap1 delta Br

Plasmid

#13255

Depositor

Titia de Lange

Article

Li et al Mol Biol Cell. 2003 Dec . 14(12):5060-8.

Insert

Repressor Activator Protein 1 delta Br (TERF2IP Human)

Use

Retroviral

Tags

FLAG

Expression

Mutation

contains hRap1 aa2-44 + 96-399 and the point muta…

Promoter

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pRK5-FLAG-SZT2 1-1021

Plasmid

#87036

Purpose

mammalian expression of SZT2 fragment

Depositor

David Sabatini

Article

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Insert

SZT2 (SZT2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

AA 1-1021

Promoter

CMV

Available since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLIK TT 3xFLAG LacZ hygro

Plasmid

#98395

Purpose

Lentiviral vector for inducible expression of LacZ with 3xFLAG N-terminal tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

LacZ

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Available since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAN19-3xFLAG-TIR1-NOSt

Plasmid

#108545

Purpose

Cloning vector including N-terminally 3xFLAG-tagged Arabidopsis auxin receptor TIR1 [Wild type] and NOS terminator

Depositor

Keiko Torii

Article

Uchida et al Nat Chem Biol. 2018 Mar;14(3):299-305. d

Insert

TRANSPORT INHIBITOR RESPONSE 1 fused with 3xFLAG and NOS terminator (TIR1 Mustard Weed)

Use

Cloning vector

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMEP5-Flag Par6 S345A

Plasmid

#24648

Depositor

Jeff Wrana

Article

Ozdamar et al Science. 2005 Mar 11. 307(5715):1603-9.

Insert

Par6 (Pard6a Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S345A

Promoter

Available since

June 2, 2010

Availability

Industry, Academic Institutions, and Nonprofits