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Plasmid#127922PurposeExpresses ShCAST and a crRNA targeting PSP1DepositorInsertShTnsB, ShTnsC, ShTniQ, ShCas12k
UseTagsExpressionMutationPromoterAvailable sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-FLEX-SaCas9-U6-sgHtr2a
Plasmid#159908PurposeMutagenesis of Htr2aDepositorInsertHtr2a (Htr2a Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAC1801_pmax-dCasRx
Plasmid#118634PurposeTransient transfection; Expresses dCasRx; CAGGS promoterDepositorInsertdCasRx
UseTagsExpressionMammalianMutationR239A/H244A/R858A/H863APromoterCAGGSAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJAT77
Plasmid#204295PurposepJAT plasmid with multiple cloning site for introducing custom payload. No U6 promoter. Restriction sites present to introduce flanking U6 promoters.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHelper_AcCAST
Plasmid#127923PurposeExpresses AcCAST and a crRNA targeting PSP1DepositorInsertAcTnsB, AcTnsC, AcTniQ, AcCas12k
UseTagsExpressionMutationPromoterAvailable sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJAT21
Plasmid#204309PurposeFor generating targeting inversions. N-terminal split GFP, expressed in abdomen and mouthpartsDepositorInsertie1-GFPn
UseCRISPRTagsExpressionMutationPromoterie1Available sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJAT25
Plasmid#204310PurposeFor generating targeting inversions. C-terminal split GFP, expressed in abdomen and mouthparts.DepositorInsertie1-GFPc
UseCRISPRTagsExpressionMutationPromoterie1Available sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
vHB16
Plasmid#193455PurposeExpression of GFP-Cas9-NLS and gRNA in A. castellaniiDepositorInserttRNA-gRNA
UseCRISPRTagsExpressionMutationPromoterU6Available sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
sgBbsI (p2Tol-U6-2xBbsI-sgRNA-HygR)
Plasmid#71485PurposeBase plasmid for sgRNA cloning with Hygromycin resistance and Tol2 transposon armsDepositorTypeEmpty backboneUseTagsExpressionMutationPromoterU6Available sinceJan. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMK33/Cas9
Plasmid#133781PurposeExpresses drosophila-optimized Cas9DepositorInsertDrosophila-optimized Cas9
UseTagsExpressionInsectMutationPromoterAvailable sinceJan. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJAT27
Plasmid#204308PurposeFor generating targeting inversions. C-terminal split GFP, expressed in flight muscles.DepositorInsertMHC-GFPc
UseCRISPRTagsExpressionMutationPromoterMHCAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJAT18
Plasmid#204307PurposeFor generating targeting inversions. N-terminal split GFP, expressed in flight muscles.DepositorInsertMHC-GFPn
UseCRISPRTagsExpressionMutationPromoterMHCAvailable sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
LRG2.1-CyOFP1
Plasmid#124771Purpose3rd generation lentiviral plasmid to co-express a guide RNA and CyOFP1DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBC2103-reci
Plasmid#117172PurposeU6-sgRNA-EFS-arC9-T2A-mCherry-P2A-Hygro; arC9 without NLSDepositorInsertreci_arCas9 without NLS
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
Construct 12 - hUBCp_Cas9_3xNLS_p2a_puroR
Plasmid#81251PurposeLentiviral construct for building stable cell lines expressing Cas9 via puromycin selectionDepositorInsertCas9-3xNLS
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAct-dCBEevoCDA1
Plasmid#195515PurposeUbiquitous expression of dCBEevoCDA1 in DrosophilaDepositorInsertdCBE-evoCDA1
UseCRISPRTagsExpressionInsectMutationPromoteract5CAvailable sinceSept. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSL3287
Plasmid#139750PurposeBase Cas12a CRISPRi plasmidDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceApril 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
GluA1 (px330:2-guideCas9)
Plasmid#169417PurposeExpression of Cas9 and 2 guidesDepositorInsertspCas9
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
rGluA2 (px330:2-guideCas9)
Plasmid#169439PurposeExpression of Cas9 and 2 guidesDepositorInsertspCas9
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
rGluA1 (px330:2-guideCas9)
Plasmid#169437PurposeExpression of Cas9 and 2 guidesDepositorInsertspCas9
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only