Showing: 3461 - 3480 of 4615 results
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EFS-SpdCas9
Plasmid#210703PurposeThis plasmid express EFS promoter driven human codon optimized SpdCas9DepositorInsertS. Pyogenes dCas9
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationD10A/H840APromoterEF1a core promoterAvailabilityAcademic Institutions and Nonprofits only -
FLAG-mFzd4-opt(40-537)-9xHis_pFastBac1
Plasmid#216378PurposeBaculovirus transfer vector to express FLAG-tagged mouse Fzd4 (codon-optimized for insect cell expression)DepositorInsertFrizzled-4 (Fzd4 Mouse)
UseBaculovirusTagsHA signal sequence-FLAGExpressionInsectMutationPromoterPolyhedrinAvailabilityAcademic Institutions and Nonprofits only -
pLX307-VHL(Y98H)-3F-BirA
Plasmid#220148PurposeExpresses VHL(Y98H)-BirA as a control for VHL substrate identification by E-STUBDepositorInsertvon Hippel-Lindau tumor suppressor (VHL Human)
UseLentiviralTags3F-BirAExpressionMammalianMutationchanged tyrosine 98 to histidine; codon optimizedPromoterEF-1aAvailabilityAcademic Institutions and Nonprofits only -
pLX307-VHL(C162F)-3F-BirA
Plasmid#220149PurposeExpresses VHL(C162F)-BirA as a control for VHL substrate identification by E-STUBDepositorInsertvon Hippel-Lindau tumor suppressor (VHL Human)
UseLentiviralTags3F-BirAExpressionMammalianMutationchanged cysteine 162 to phenylalanine; codon opti…PromoterEF-1aAvailabilityAcademic Institutions and Nonprofits only -
PD2529 human beta1 full
Plasmid#221401PurposeMammalian expression of human integrin beta1 full-lengthDepositorInsertintegrin beta1 full-length (ITGB1 Human)
UseTagsCD33 secretion peptide (MPLLLLLPLLWAGALA) and HA …ExpressionMammalianMutationcodon-optimized mature sequencePromoterAvailabilityAcademic Institutions and Nonprofits only -
pJL023
Plasmid#122841Purposep300 core domain with GreenGate D-E flanking sequencesDepositorInsertCore domain of p300 H3K27 histone acetyltransferase, codon-optimised for Arabidopsis
UseGolden gate compatible cloning vectorTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pJL035
Plasmid#122854PurposeG9a SET domain with GreenGate D-E flanking sequencesDepositorInsertG9a SET catalytic domain, codon-optimised for Arabidopsis
UseGolden gate compatible cloning vectorTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pMH220
Plasmid#122836PurposedCas9 with GreenGate B-C flanking sequencesDepositorInsertNuclease-dead SpCas9 (dCas9), codon-optimised for Arabidopsis
UseGolden gate compatible cloning vectorTagsExpressionMutationContains D10A and H840A mutationsPromoterAvailabilityAcademic Institutions and Nonprofits only -
XX01CAPN1A-c001
Plasmid#175491PurposeProtein expression in bacterial cells. Entire CDS, M1-A714 (CAPN1); EF hand 1-5, H86-S268 (CAPNS1). CAPN1 can be biotinylated. Codon optimised.DepositorInsertCAPN1 / CAPNS1 (CAPN1 Human)
UseTagsAviTag and His6-TEVExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
XX01CAPN2A-c001
Plasmid#175492PurposeProtein expression in bacterial cells. Entire CDS, M1-L700 (CAPN2); EF hand 1-5, H86-S268 (CAPNS1). CAPN2 can be biotinylated. Codon optimised.DepositorInsertCAPN2 / CAPNS1 (CAPN2 Human)
UseTagsAviTag and His6-TEVExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
MSCV-opEBNA2-IRES-mCherry
Plasmid#220355PurposeExpresses EBV latent gene EBNA2 in mammalian cells (EBNA2 coding sequence was optimised for expression)DepositorInsertopEBNA2 (EBNA-2 Epstein-Barr Virus (EBV) strain B95-8)
UseRetroviralTagsExpressionMammalianMutationcodon-optimised for expression in mammalian cellsPromoterAvailabilityAcademic Institutions and Nonprofits only -
SFG.wtCNb_opt.IRES.eGFP
Plasmid#22492DepositorInsertCodon optimised wild type Calcineurin B (PPP3R1 Human)
UseRetroviralTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pICSL80005
Plasmid#50322DepositorInsertCDS, variant of turboGFP codon-optimised for plants (Pontellina plumata)
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pICSL80004
Plasmid#50325DepositorInsertCDS, plant codon-optimsed variant of turbo RFP (Entacmaea quadricolor)
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
SFG.CNb30_opt.IRES.eGFP
Plasmid#22493DepositorInsertCodon optimised Calcineurin B mutant 30 (PPP3R1 Human)
UseRetroviralTagsExpressionMutationL124T; K125-LA-InsPromoterAvailabilityAcademic Institutions and Nonprofits only -
pRPF185
Plasmid#106367PurposeC. difficile inducible expression system. Shuttle plasmid containing a tetracycline-inducible gusADepositorInsertTerm(fdx)-Ptet-gusA-Term(slpA) (gusA )
UseE. coli - c. difficile shuttle vectorTagsExpressionMutationcodon optimised for C. difficilePromoterPtetAvailabilityAcademic Institutions and Nonprofits only -
pRPF144
Plasmid#106372PurposeC. difficile constitutive expression system. Shuttle plasmid containing constitutive Pcwp2 -gusADepositorInsertPcwp2-gusA (gusA )
UseE. coli - c. difficile shuttle vectorTagsExpressionMutationcodon optimised for C. difficilePromoterPcwp2AvailabilityAcademic Institutions and Nonprofits only -
pJAID6A
Plasmid#165046PurposeIntegrative expression of yEGFP and rice auxin receptor gene OsTIR1. OsTIR1 is codon-optimised for expression in S. cerevisiaeDepositorInsertP-URA3>KlURA3>TAgTEF1-P-TEF1>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
UseTagsExpressionYeastMutationWTPromoterAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_GEM2-Halo-FRB
Plasmid#197056PurposeExpression of Synechococcus elongatus encapsulin Halo-FRB-fusion in mammalian cellsDepositorInsertEncapsulin
UseTagsFRB and HaloTagExpressionMammalianMutationCodon-optimised for human cell expressionPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 3461 - 3480 of 4615 results