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Plasmid#47364PurposeBmal fragment cloned with Venus tag and used for BiFCDepositorInsertBMAL (Bmal1 Mouse)
UseTagsVenus aa 156-239ExpressionMammalianMutationaa 1-465PromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
EGFP-hPMCA2x/b delta6
Plasmid#47587DepositorInsertPMCA2x/b delta6 (ATP2B2 Human)
UseTagsEGFP-HA-HisExpressionMammalianMutation6 C-terminal AA deleted (needed for PDZ binding)PromoterCMVAvailable sinceOct. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-Synaptojanin-1-145
Plasmid#223563PurposeExpresses the short isoform of SYNJ1 (neuronal) with mCherry tagged at the n-terminusDepositorInsertSYNJ1 (SYNJ1 Human)
UseTagsHis and mCherryExpressionMammalianMutationPromoterAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Sec22b-shR
Plasmid#208358PurposeMammalian expression of fluorescent N-terminally tagged shRNA resistant Sec22bDepositorInsertSec22b (Sec22b Rat)
UseTagsEGFPExpressionMammalianMutationfour silent mutations introduced to render shRNA …PromoterCMVAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP-Bcl-2-K17R-K22R
Plasmid#220294Purposemammalian expression of Bcl-2-K17R-K22R tagged with GFPDepositorInsertBcl2 (BCL2 Human)
UseTagsEmerald GFPExpressionMammalianMutationK17R-K22RPromoterCMVAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP-Bcl-2-G128L-A131L-T132L
Plasmid#220304Purposemammalian expression of Bcl-2-G128L-A131L-T132L tagged with GFPDepositorInsertBcl2 (BCL2 Human)
UseTagsEmerald GFPExpressionMammalianMutationG128L-A131L-T132LPromoterCMVAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-PRKCZ-C20 (N-PRKCZ-C20)
Plasmid#217762PurposeFluorescent reporter for ceramide (putative, mammalian expression)DepositorInsertProtein kinase C zeta (PRKCZ Human)
UseTagsEGFPExpressionMammalianMutationLinker GGSSGGGA plus C20 domain (aa 405-592)PromoterCMVAvailable sinceJune 11, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEGFP-RepoMan-RATA
Plasmid#184270PurposeExpresses shRNA resistant GFP-RepoMan RATA MutantDepositorInsertcell division cycle associated 2 (CDCA2 Human)
UseTags3xHA and GFPExpressionMammalianMutationV383A, F395APromoterCMVAvailable sinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Repo-Man-1-890
Plasmid#184272PurposeExpresses C-terminally truncated GFP-RepoManDepositorInsertcell division cycle associated 2 (CDCA2 Human)
UseTagsGFPExpressionMammalianMutationtruncated Repo-Man after aminoacid 893PromoterCMVAvailable sinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-RepoMan-T394A
Plasmid#204994PurposeExpresses shRNA resistant GFP-RepoMan T394A MutantDepositorInsertcell devision cycle associated 2 (CDCA2 Human)
UseTagsGFPExpressionMammalianMutationchanged Threonine 394 to AlaninPromoterCMVAvailable sinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-RepoMan-S893D
Plasmid#204995PurposeExpresses shRNA resistant GFP-RepoMan S893D MutantDepositorInsertcell division cycle associated 2 (CDCA2 Human)
UseTagsGFPExpressionMammalianMutationchanged Serine 893 to Aspartic acidPromoterCMVAvailable sinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-ORP8-H514A-H515A
Plasmid#208360PurposeMammalian expression of fluorescent N-terminally tagged H514A-H515A mutant of ORP8DepositorInsertORP8 (OSBPL8 Human)
UseTagsEGFPExpressionMammalianMutationH514A-H515APromoterCMVAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-p62-TIR-AAA
Plasmid#187901PurposeMammalian cell expression of a triple mutant of p62: P228A, E230A, P232ADepositorInsertSQSTM1 (SQSTM1 Human)
UseTagsmCherryExpressionMammalianMutationTriple alanine mutant: P228A, E230A, P232APromoterCMVAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
Halotag-6xHp
Plasmid#214414PurposeLipid droplet localization of 6xHp of Spastin fused to HalotagDepositorInsert6x Spastin aa 43-92 (SPAST Human)
UseTagsHaloExpressionMammalianMutationPromoterCMVAvailable sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
mApple-1xHp
Plasmid#214409PurposeLipid droplet and ER localization of 1xHp of Spastin fused to mAppleDepositorInsertSpastin aa 43-92 (SPAST Human)
UseTagsmAppleExpressionMammalianMutationPromoterCMVAvailable sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003(V114T, V116T)-Talin rod-mCherry
Plasmid#210027PurposeExpression of SpyTag003(V114T, V116T)-Talin rod(434-2541)-mCherry in mammalian cellsDepositorInsertSpyTag003(V114T,V116T)-Talin1 rod domain(434-2541)-mCherry
UseTagsExpressionMammalianMutationSpyTag003 V114T and V116T mutations to modify Spy…PromoterCMVAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENeCFPCNAL2 pc922
Plasmid#167563PurposeFor construction of CFP-tagged human PCNA, the GFP coding sequence in the pENeGFPCNAL2 vector was replaced by the ECFP coding sequence from the pECFP-C1 vector (Clontech Laboratories, Inc., CA).DepositorInsertsUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
mEos3.2trLATm2allL+A
Plasmid#203750PurposeEncodes the transmembrane domain from human LAT with mEos3.2 fused on the C terminus. Residues within the transmembrane domain mutated to L and A to increase the TMD interfacial surface area with the membrane. Used as a fluorescent plasma membrane probe.DepositorInserttrLATm2(allL+A) (LAT Human)
UseTagsmEos3.2ExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEB1N-mApple-LZ-LOV2
Plasmid#197418PurposeMammalian expression of the mApple-tagged N-terminal half of photo-inactivated π-EB1DepositorInsertMAPRE1 (MAPRE1 Human)
UseTagsA. sativa phototropin 1 LOV2 domain, GCN4 leucine…ExpressionMammalianMutationEB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…PromoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRS140
Plasmid#197141PurposescCloneSelect C-to-T reporter encoding BC-C1 barcode (CMV promoter version)DepositorInsertEGFP
UseLentiviralTagsExpressionMammalianMutationEGFP(M1V)PromoterCMVAvailable sinceMay 30, 2023AvailabilityAcademic Institutions and Nonprofits only