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Plasmid#162501PurposeExpresses a ST6GAL1 mutant with a GFP tag in its C-terminus in mammalian cells. The stem region of Tac is inserted within ST.DepositorUseTagsGFPExpressionMammalianMutationThe Tac stem region is inserted within ST6Gal1PromoterAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only
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Tac(5A)-GFP
Plasmid#162495PurposeExpresses C-terminally GFP tagged Tac with mutated five O-glycosylation sites in mammalian cellsDepositorInsertTac with five threonine residues mutated into alanine residues in the stem region (IL2RA Human)
UseTagsGFPExpressionMammalianMutationThe five threonine residues are mutated into alan…PromoterAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-Tac(5A)
Plasmid#162493PurposeExpresses GFP tagged Tac with mutated five O-glycosylation sites in mammalian cellsDepositorInsertTac with five threonine residues mutated into alanine residues in the stem region (IL2RA Human)
UseTagsGFPExpressionMammalianMutationThe five threonine residues are mutated into alan…PromoterAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
Nup54-mEGFP494(2)
Plasmid#163428PurposeMammalian expression of Nup54 with a mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 7 amino acids at the carboxyl end of the mEGFPDepositorInsertNup54 (NUP54 Human)
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-rUSP19CYT
Plasmid#155248Purposefor mammalian overexpression of the CYT isoform of rat USP19 fused to GFP at N-terminusDepositorInsertUSP19 (Cytoplasmic isoform) (Usp19 Rat)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-rUSP19ER
Plasmid#155247Purposefor mammalian overexpression of the ER isoform of rat USP19 fused to GFP at N-terminusDepositorInsertUSP19 (ER isoform) (Usp19 Rat)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLz26_CsoS2_N1-N2_GFP
Plasmid#140851PurposeHis-tagged first two N-peptides of NTD fused to Superfolder GFP ([N1-N2]-GFP) used for MST, pET-14b backboneDepositorInsertCsoS2 NTD repeats 1-2 fused to GFP
UseTagsHexahistidine tag, Strep-Tag II, and Superfolder …ExpressionBacterialMutationPromoterT7Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFE-sfGFP
Plasmid#132998PurposepFE carrying a sfGFP geneDepositorInsertsfGFP
UseTags6xhisExpressionBacterialMutationPromotertetRAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3-FeGFP
Plasmid#138261PurposeDonor-eGFP vector to be used in plasmid to plasmid integration reporter assay. eGFP is conditionally expressed when integrated at iCas9-sites on acceptor plasmidDepositorInserteGFP
UseCRISPRTagsExpressionMammalianMutationPromoterNoneAvailable sinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
ID1GFP Donor
Plasmid#119282PurposeMouse ID1-eGFP for HR to create reporterDepositorInsertId1GFP + homology arms (Id1 Mouse)
UseReporterTagseGFPExpressionMutationPromoterAvailable sinceJan. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pStrep-5xEGFP-N1
Plasmid#122492PurposeExpression of Strep-tagged 5xEGFPDepositorInsertStrep-tagged 5xEGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pStrep-7xEGFP-N1
Plasmid#122494PurposeExpression of Strep-tagged 7xEGFPDepositorInsertStrep-tagged 7xEGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pStrep-8xEGFP-N1
Plasmid#122495PurposeExpression of Strep-tagged 8xEGFPDepositorInsertStrep-tagged 8xEGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pStrep-9xEGFP-N1
Plasmid#122496PurposeExpression of Strep-tagged 9xEGFPDepositorInsertStrep-tagged 9xEGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pStrep-10xEGFP-N1
Plasmid#122497PurposeExpression of Strep-tagged 10xEGFPDepositorInsertStrep-tagged 10xEGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pStrep-11xEGFP-N1
Plasmid#122498PurposeExpression of Strep-tagged 11xEGFPDepositorInsertStrep-tagged 11xEGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pStrep-12xEGFP-N1
Plasmid#122499PurposeExpression of Strep-tagged 12xEGFPDepositorInsertStrep-tagged 12xEGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
25Q-ymsfGFP
Plasmid#128559PurposeHttex1 (without proline-rich region) fused to ymsfGFPDepositorInsert25Q-Httex1 (without proline-rich region) fused to ymsfGFP
UseTagsymsfGFPExpressionYeastMutationPromoterGal1Available sinceAug. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJUMP49-2D'(sfGFP)
Plasmid#126994PurposeLevel 2 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJUMP38-1A(sfGFP)
Plasmid#126984PurposeLevel 1 vector with alternative selection marker (chloramphenicol). Origin pUC (high copy number). sfGFP cloning reporter.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only