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Plasmid#124477PurposeFor CRISPR-mediated epigenome editing of human BLK gene locusDepositorInsertBLK_E2-4_gRNA (BLK Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSPgRNA_BLK_E3-3
Plasmid#124484PurposeFor CRISPR-mediated epigenome editing of human BLK gene locusDepositorInsertBLK_E3-3_gRNA (BLK Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLL5.0 CortW22A mCherry
Plasmid#187268PurposeLenticiral expression of mCherry-Cortactin W22ADepositorInsertCortactin (CTTN Human)
UseLentiviralTagsmcherryExpressionMutationW22APromoter5' LTRAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
BMP10 N67Q N131Q D4G (S2-2)
Plasmid#184622Purposefor transfection into S2 cells and expression of BMP10 proteinDepositorInsertBMP10 (BMP10 Human)
UseTags8x His - streptavidin binding peptideExpressionInsectMutationN67Q, N131Q, ARIRR(312-316) replaced with GPromoterAvailable sinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1C-Fluc-CG1856_A
Plasmid#145905PurposeInsect Expression of CG1856-3UTRDepositorInsertCG1856-3UTR (ttk Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSP72-RBM8A-3'UTR AluY-Sz mut 2598-4066
Plasmid#175586Purposein vitro transcription of mut RBM8A 3'UTRDepositorInsertRBM8A-3'UTR AluY-Sz mut 2598-4066
UseIn vitro transcriptionTagsExpressionMutationPromoterT7Available sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-BLAST TFR1
Plasmid#169890PurposeExpress TFR1 open reading frame. Lacks IRE regulatory elementsDepositorInsertTFRC (TFRC Human)
UseRetroviralTagsExpressionMammalianMutationTFRC cDNA, lacking 3'UTR regulatory elementsPromoterAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHA853 - mec-4p::Fynempty
Plasmid#139210PurposeControl vector for Fyn kinase expression in mec-4 neuronsDepositorInsertFyn (FYN Human)
UseTagsExpressionWormMutationdeletion and frame shift to prevent Fyn expressionPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHA850 - osm-10p::FynY531F
Plasmid#139207PurposeExpress activated Fyn kinase in osm-10 neuronsDepositorInsertFyn (FYN Human)
UseTagsExpressionWormMutationY531FPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHA851 - osm-10p::Fynempty
Plasmid#139208PurposeControl vector for Fyn kinase expression in osm-10 neuronsDepositorInsertFyn (FYN Human)
UseTagsExpressionWormMutationdeletion and frame shift to prevent Fyn expressionPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
FUS_LC_withR
Plasmid#139128Purposeexpress FUS LC with the same number and spacing of arginines as hnRNPA2 LCDepositorInsertFUS (FUS Human)
UseTagsExpressionBacterialMutationadd argininesPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
hnRNPA2_LC_nocharge_wR
Plasmid#139119Purposeexpress hnRNPA2 LC with no charged residues with R added backDepositorInserthnRNPA2 (HNRNPA2B1 Human)
UseTagsExpressionBacterialMutationremove all K, most DPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
FUS_LC_charge
Plasmid#139126Purposeexpress FUS LC with an hnRNPA2 LC-like charge patterningDepositorInsertFUS (FUS Human)
UseTagsExpressionBacterialMutationAdd R, K, D to make FUS LC have similar charge pa…PromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJWK_VD_04
Plasmid#158105PurposeCaV1.3 5'UTR luciferase reporterDepositorInsert5'UTR of CACNA1D (uc003dgu.5)
UseLuciferaseTagsExpressionMammalianMutationPromoterPGKAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJWK_VD_03
Plasmid#158104PurposeCaV1.2 5'UTR luciferase reporterDepositorInsert5'UTR of CACNA1C (uc009zdu.1)
UseLuciferaseTagsExpressionMammalianMutationPromoterPGKAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
Met133I, M1381I, M186I ELL2
Plasmid#127263PurposeFor in vitro translation of human ELL2 with Met133I, M1381I, M186IDepositorInsertELL2 (Met133ILeu, M1381ILeu, M186ILeu) (ELL2 Human)
UseIn vitro translationTagsExpressionMutationThree Mets (133, 138, 186) to IleuPromoterT7Available sinceNov. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA tagged Sph ELL2
Plasmid#127270PurposeFor in vitro translation of human WT ELL2 with internal HA tagDepositorInsertELL2 (ELL2 Human)
UseIn vitro translationTagsExpressionMutationWT Mets and HA tag at SphI site (8th Met)PromoterT7Available sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Met138ILeu ELL2
Plasmid#127260PurposeFor in vitro translation of human ELL2 with Met138 mutated to Ileu to ask if there is internal initiationDepositorInsertELL2 (Met138 to Ileu) (ELL2 Human)
UseIn vitro translationTagsExpressionMutationMet138 to Ileu QuikchangePromoterT7Available sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Met186ILeu ELL2
Plasmid#127261PurposeFor in vitro translation of human ELL2 with Met186 mutated to Ileu to ask if there is internal initiationDepositorInsertELL2 (Met186 to Ileu) (ELL2 Human)
UseIn vitro translationTagsExpressionMutationMet186 to Ileu QuikchangePromoterT7Available sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Met138ILeu, Met186ILeu ELL2
Plasmid#127265PurposeFor in vitro translation of human ELL2 with Met138ILeu, Met186ILeuDepositorInsertELL2 (Met138ILeu, Met186ILeu) (ELL2 Human)
UseIn vitro translationTagsExpressionMutationTwo Mets (138,186) to ILeu QuikchangePromoterT7Available sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only