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MSCV-puro-N3FLAG-MYB-FL

Plasmid

#105596

Purpose

retrovirally express mouse MYB with 3*FLAG tag at N terminal and puro resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

Full length MYB (mouse) (Myb Mouse)

Use

Retroviral

Tags

3*FLAG

Expression

Mammalian

Mutation

Promoter

MSCV-LTR

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-Flag-YAP-S384A

Plasmid

#33070

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2010 Jan 1. 24(1):72-85.

Insert

YAP (YAP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

S384A

Promoter

CMV

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pLJC6-Flag CASTOR1 I280A

Plasmid

#84497

Purpose

Lentiviral expression of CASTOR2 I280A mutant

Depositor

David Sabatini

Article

Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

Insert

CASTOR1 I280A (GATSL3 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

I280A

Promoter

sestrin2

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only
pMSCV-puro-Flag-mMcl-1 KR

Plasmid

#32983

Depositor

Joseph Opferman

Article

Stewart et al Mol Cell Biol. 2010 Jun;30(12):3099-110.

Insert

Mcl-1 (Mcl1 Mouse)

Use

Retroviral

Tags

3XFlag

Expression

Mammalian

Mutation

All Lys to Arg

Promoter

LTR

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
Brm BDmut-Flag pShuttle

Plasmid

#122108

Purpose

Expression of full length Brm containing point mutations in its bromodomain in mammalian cells

Depositor

Paul Herring

Article

Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

Insert

Brm (SMARCA2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pFlag-CMV2-Brd4 R1337P

Plasmid

#21939

Depositor

Eric Verdin

Article

Bisgrove et al Proc Natl Acad Sci U S A. 2007 Aug 21. 1

Insert

BRD4 (BRD4 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

aa 1209-1362 only, R1337P

Promoter

Available since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only
pSG5-Myr-FLAG-p110β-DM

Plasmid

#55719

Purpose

eukaryotic expression of myristoylation tag (Myr-FLAG) p110b RBD double mutant S205D, K224A

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

p110 beta

Use

Tags

myristoylation tag (Myr-FLAG)

Expression

Mammalian

Mutation

S205D, K224A

Promoter

T7

Available since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
Hy_pMT Flag-UAP56 MUT

Plasmid

#110124

Purpose

Expresses Flag-tagged human UAP56 (DDX39B) with 4 mutations that impact circRNA nuclear export activity

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

UAP56 (DDX39B Human)

Use

Tags

Flag

Expression

Insect

Mutation

4 mutations (KSLN motif changed to RSFS)

Promoter

Metallothionein Promoter (pMT)

Available since

Sept. 27, 2020

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-MKL1 D381-506

Plasmid

#19853

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Aug 11. ():.

Insert

MKL1 (MRTFA Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

deleted amino acids 381 to 506

Promoter

Available since

March 16, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/Flag-hINO80N1 (1-262)

Plasmid

#29441

Depositor

Joan Conaway, Ronald Conaway

Article

Chen et al J Biol Chem. 2011 Feb 8. ():.

Insert

INO80 (INO80 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

INO80 1-262 (a.a.)

Promoter

Available since

Aug. 1, 2011

Availability

Academic Institutions and Nonprofits only
pCI-Flag-NEDD4L∆WW2

Plasmid

#166708

Purpose

expresses NEDD4L with inactivating mutation in second WW domain in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

Insert

NEDD4L W453A

Use

Tags

FLAG

Expression

Mammalian

Mutation

changed tryptophan 453 to alanine

Promoter

CMV promoter

Available since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCI-Flag-NEDD4L∆WW3

Plasmid

#166709

Purpose

expresses NEDD4L with inactivating mutation in third WW domain in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

Insert

NEDD4L W525A

Use

Tags

FLAG

Expression

Mammalian

Mutation

changed tryptophan 525 to alanine

Promoter

CMV promoter

Available since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hRIPK1-Flag-K599R

Plasmid

#112490

Purpose

Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.

Depositor

Junying Yuan

Insert

RIPK1 (RIPK1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

K599R

Promoter

Available since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Flag-PGC-1alpha2

Plasmid

#45502

Depositor

Jorge Ruas, Bruce Spiegelman

Article

Ruas et al Cell. 2012 Dec 7;151(6):1319-31. doi: 10

Insert

PGC-1alpha Isoform2 (Ppargc1a Mouse)

Use

Tags

6xHis and Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV6-XL4 FLAG-TMEFF2-Fc

Plasmid

#115774

Purpose

Expression of the extracellular domain of TMEFF2 with N-terminal FLAG tag and C-terminal hIgG tag.

Depositor

Davide Comoletti

Insert

TMEFF2 (TMEFF2 Human)

Use

Tags

FLAG and hIgG/Fc

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

Oct. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-FLCN (C terminus)

Plasmid

#72296

Purpose

overexpression

Depositor

David Sabatini

Article

Tsun et al Mol Cell. 2013 Nov 21;52(4):495-505. doi

Insert

FLCN C-terminus (FLCN Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Contains only C terminus of FLCN as defined in PM…

Promoter

CMV

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
1572 pSG5L Flag HA GGA2

Plasmid

#11184

Depositor

William Sellers

Insert

GGA2 (GGA2 Human)

Use

Tags

HA and flag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2006

Availability

Academic Institutions and Nonprofits only
pLPC FLAG hRap1 delta CT

Plasmid

#13252

Depositor

Titia de Lange

Article

Li et al Mol Biol Cell. 2003 Dec . 14(12):5060-8.

Insert

Repressor Activator Protein 1 delta CT (TERF2IP Human)

Use

Retroviral

Tags

FLAG

Expression

Mutation

contains hRap1 aa2-270 + 331-399 and the point mu…

Promoter

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 2xFLAG-2xSTREP EMI1

Plasmid

#158947

Purpose

Express human EMI1 in mammalian cells

Depositor

Michele Pagano

Article

Marzio et al Mol Cell. 2019 Jan 17;73(2):224-237.e6.

Insert

EMI1 (FBXO5 Human)

Use

Tags

2X FLAG 2X STREP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV10/3xflag-Clock L57E

Plasmid

#47337

Purpose

Mutation used for co-IPs

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

Clock (Clock Mouse)

Use

Tags

3x Flag

Expression

Mammalian

Mutation

L57E

Promoter

CMV

Available since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only