Addgene

Showing: 3441 - 3460 of 12138 results

Results per page:

Sort results by:

pCAG-SRGAP1FBAR(516a.a.)-EGFP

Plasmid

#168505

Purpose

Expression of F-BAR domain (aa1-516) of SRGAP1 with a C-terminal EGFP fusion.

Depositor

Franck Polleux

Article

Coutinho-Budd et al J Cell Sci. 2012 Jul 15;125(Pt 14):3390-

Insert

SRGAP1-FBAR (SRGAP1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

aa 1-516

Promoter

CAG

Available since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-phSyn-flex-SypEGFP

Plasmid

#153203

Purpose

Can be used to generate AAV virus that will mark the presynaptic terminal (synaptophysin-fused) EGFP in the presence of Cre in neurons from the synapsin promoter

Depositor

Lynette Lim

Insert

synaptophysin-EGFP

Use

AAV

Tags

Expression

Mutation

Promoter

rat synapsin

Available since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-EGFP-C1-Hs4E-T_AF

Plasmid

#148687

Purpose

Mammalian Expression of Hs4E-T

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

Hs4E-T (EIF4ENIF1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N1-human lyn–GFP

Plasmid

#35958

Purpose

Expresses human lyn protein codon optimized for zebrafish expression.

Depositor

Anna Huttenlocher

Article

Yoo et al Nature. 2011 Nov 20;480(7375):109-12. do

Insert

LYN (LYN Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

wt

Promoter

cmv

Available since

April 27, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFPC1-FLAG-Synaptojanin 1-170

Plasmid

#22294

Depositor

Pietro De Camilli

Article

Perera et al Proc Natl Acad Sci U S A. 2006 Dec 19. 1

Insert

Synaptojanin 1_170 (SYNJ1 Human)

Use

Tags

Flag and GFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only
pEGFP_sfCherry2(11)_Clathrin light chain

Plasmid

#83032

Purpose

Expresses sfCherry2(11) tagged clathrin light chain in mammalian cells

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

sfCherry2(11)_Clathrin light chain (CLTA Human, Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pHX_HDR_hPAX7_IRES-EGFP-PGK-Neo

Plasmid

#160458

Purpose

Homology-directed recombination donor vector for EGFP reporter knock-in to human PAX7 3' UTR

Depositor

April Pyle

Article

Xi et al STAR Protoc. 2020 Nov 5;1(3):100158. doi

Inserts

PAX7 Left/5' Homology Arm (PAX7 Human)
PAX7 Right/3' Homology Arm (PAX7 Human)

Use

Insertion of a fluorescent reporter through homol…

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBMN-2xFKBP-mEGFP-NDP52

Plasmid

#110465

Purpose

retrovirus construct for stable expressing mEGFP tagged NDP52

Depositor

Richard Youle

Article

Lazarou et al Nature. 2015 Aug 20;524(7565):309-14. do

Insert

NDP52 (CALCOCO2 Human)

Use

Retroviral; Retrovirus

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
FU_tetO_dCas9-VP192-T2A-EGFP

Plasmid

#121173

Purpose

Lentiviral construct for inducible expression of dCas9-VP192 transcriptional activator and EGFP.

Depositor

Timo Otonkoski

Insert

dCas9-VP192-T2A-EGFP

Use

CRISPR and Lentiviral; Inducible by doxycycline

Tags

T2A-EGFP-ires-puro

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
BE3-P2A-EGFP (pJUL977)

Plasmid

#123612

Purpose

CAG promoter expression plasmid for rAPOBEC1-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP.

Depositor

Keith Joung

Article

Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

Insert

BE3-P2A-EGFP

Use

Tags

Expression

Mammalian

Mutation

D10A in SpCas9

Promoter

CAG

Available since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-TDsmURFP-IRES-eGFP

Plasmid

#80344

Purpose

Expresses Tandem Dimer smURFP and eGFP in mammalian cells.

Depositor

Erik Rodriguez, Roger Tsien

Article

Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

Inserts

Tandem Dimer smURFP
eGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and IRES

Available since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-ninein coiled-coil 2

Plasmid

#73522

Purpose

Encodes the second coiled-coil domain of mouse ninein with an EGFP tag at its N-terminus

Depositor

Michel Bornens

Article

Delgehyr et al J Cell Sci. 2005 Apr 15;118(Pt 8):1565-7

Insert

ninein coiled-coil 2 (Nin Mouse)

Use

Tags

EGFP tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 13, 2016

Availability

Academic Institutions and Nonprofits only
pEGFP-ninein coiled-coil 1

Plasmid

#73521

Purpose

Encodes the first coiled-coil domain of mouse ninein with an EGFP tag at its N-terminus

Depositor

Michel Bornens

Article

Delgehyr et al J Cell Sci. 2005 Apr 15;118(Pt 8):1565-7

Insert

ninein coiled-coil 1 (Nin Mouse)

Use

Tags

EGFP tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 29, 2016

Availability

Academic Institutions and Nonprofits only
pLenti-PGK-EGFPc3-Neo

Plasmid

#189741

Purpose

The construct was used to express GFP in mammalian cells as the control for the analysis of the subcellular location of this protein.

Depositor

Robert Sobol

Article

Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

Insert

EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-FLEXfrt-EGFP-KASH

Plasmid

#154374

Purpose

Vector for Flp-dependent expression of EGFP tagged KASH protein domain

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

EGFP-KASH

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pPB-TetON-mEGFP-HMGB1-WT

Plasmid

#194561

Purpose

Dox-inducible Mammalian Expression of mEGFP-HMGB1 WT IDR Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pFH2.16_MiniGagZip-MCP_Zip-P2A-eGFP

Plasmid

#205531

Purpose

Express HIV MiniGagZip-MCP_Zip-P2A-eGFP

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

MiniGagZip-MCP_Zip

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
T/Brachyury-eGFP Rex Neo

Plasmid

#21222

Depositor

Mark Mercola

Article

Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

Insert

Brachyury promoter

Use

Lentiviral

Tags

eGFP

Expression

Mutation

Promoter

Available since

July 1, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
AICSDP-116:PCNA-mEGFP

Plasmid

#170818

Purpose

Homology arms and mEGFP-linker sequence for N-terminus tagging of human PCNA

Depositor

Allen Institute for Cell Science

Insert

PCNA Homology Arms with mEGFP-linker (PCNA Human)

Use

CRISPR; Donor template

Tags

mEGFP-linker

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Promoter

Available since

June 11, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
3xFLAG-dCas9/MSCV-EGFP

Plasmid

#82613

Purpose

Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

Depositor

Hodaka Fujii

Article

Fujita et al Genes Cells. 2018 Feb 26. doi: 10.1111/g

Insert

3xFLAG-dCas9

Use

Retroviral

Tags

3xFLAG tag and NLS (nuclear localization signal)

Expression

Mutation

human codon-optimized, D10A + H840A

Promoter

LTR

Available since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge