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pOPINK-OTUD3 (OTU, aa 52-209)

Plasmid

#61412

Purpose

Expresses human OTUD3 (OTU domain) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD3 (OTUD3 Human)

Use

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-51 and aa 210-398.

Promoter

T7

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-AuroraB kd K106R

Plasmid

#108493

Purpose

expression of EGFP-AuroraB kinase dead (K106R)

Depositor

Susanne Lens

Article

Hengeveld et al Mol Cell Proteomics. 2012 May;11(5):47-5

Insert

AuroraB (AURKB Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Kinase death K106A

Promoter

CMV

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX6p1-Rbck1

Plasmid

#63135

Purpose

Expression of human RBCK1/HOIL-1L

Depositor

Titia Sixma

Article

Smit et al EMBO J. 2012 Oct 3;31(19):3833-44. doi:

Insert

HOIL-1L (RBCK1 Human, Synthetic)

Use

Tags

GST

Expression

Bacterial

Mutation

Synthetic based on human protein sequence

Promoter

tac

Available since

March 13, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA-c/o NL4-3 Env delta CT

Plasmid

#139285

Purpose

HIV-1 NL4-3 glycoprotein lacking cytoplasmic tail

Depositor

Jeremy Luban

Article

Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

Insert

codon optimized HIV-1 Env delta cytoplasmic tail

Use

Tags

Expression

Mammalian

Mutation

deletion of cytoplasmic tail after residue 710

Promoter

CMV IE

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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PIK3CA - Exon9 E545K

Plasmid

#16642

Depositor

Bert Vogelstein

Article

Samuels et al Science. 2004 Apr 23. 304(5670):554.

Insert

PIK3CA E545K (PIK3CA Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

E545K; see note below

Promoter

Available since

March 10, 2008

Availability

Academic Institutions and Nonprofits only

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pOPINK-OTUD2 (full-length, aa 1-348)

Plasmid

#61409

Purpose

Expresses human OTUD2 (full-length) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD2 (YOD1 Human)

Use

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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pOPINK-Cezanne (OTU, aa 53-446)

Plasmid

#61581

Purpose

Expresses human Cezanne (OTU domain) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

Cezanne (OTUD7B Human)

Use

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-52 and aa 447-843.

Promoter

T7

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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SLK_HUMAN_D0

Plasmid

#79733

Purpose

This plasmid encodes the kinase domain of SLK. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

Markus Seeliger, John Chodera, Nicholas Levinson

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

SLK (SLK Human)

Use

Tags

His10-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pROF141

Plasmid

#155329

Purpose

Binary vector for expression of the PULSE system under the control of PAtUbi10 promoter

Depositor

Matias Zurbriggen

Article

Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

Insert

LB_PAtUbi10-SRDX-NLS-EL222-Tnos_SF_PAtUbi10-E-PIF6-NLS-Tnos_PAtUbi10-PhyB-VP16-NLS-Tnos_RB

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pROF402

Plasmid

#155334

Purpose

Binary vector for expression of FLuc controlled by the blue light-repressible system

Depositor

Matias Zurbriggen

Article

Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

Insert

LB_P35Senhancer(-953 to -51)-(C120)5-PhCMVmin-FLuc-T35S_RB

Use

Luciferase and Synthetic Biology; Eukaryotic expr…

Tags

Expression

Mutation

Promoter

Available since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pROF441

Plasmid

#155336

Purpose

Binary vector for expression of a gRNA targeting AtAP1 promoter

Depositor

Matias Zurbriggen

Article

Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

Insert

LB_PAtU6-26-gRNA(PAtAP1)-sgRNA_RB

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pCsy_complex

Plasmid

#89232

Purpose

Plasmid contains the genes Csy1, Csy2, Csy3, Csy4, cloned as a polycistronic operon from bacterium Pseudomonas aeruginosa PA14. Csy3 includes an N-terminal 6his tag and TEV protease cleavage site

Depositor

Blake Wiedenheft

Article

Chowdhury et al Cell. 2017 Mar 23;169(1):47-57.e11. doi:

Inserts

Csy1
Csy2
Csy3
Csy4

Use

Tags

6xHis-TEV protease

Expression

Mutation

Promoter

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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TRE-UBC-H2-Kb SCT-T2A-GFP

Plasmid

#205454

Purpose

lentiviral plasmid for expression of mouse H2-Kb SCT

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

H2-Kb SCT (H2-K1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

UBC

Available since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pGL4.23 WT 2014 EBOV + Mucin-Like Domain

Plasmid

#85794

Purpose

Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

Depositor

Pardis Sabeti, Jeremy Luban

Article

Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

Insert

Makona Ebola Virus Glycoprotein

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV IE

Available since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-PP-human-CHMP3

Plasmid

#154180

Purpose

expresses human CHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

CHMP3 (CHMP3 Human)

Use

Tags

FLAG and Strep-Tag II

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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AttB_[Kozak-mut]ACE2_IRES-mCherry-H2A-P2A-PuroR

Plasmid

#171595

Purpose

Weak human ACE2 abundance from Bxb1 landing pad

Depositor

Kenneth Matreyek

Article

Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

Inserts

ACE2 (ACE2 Human)
mCherry
H2A
Pac

Use

Synthetic Biology; Promoterless bxb1 dna recombin…

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pXL003-ishRNA-beta-catenin-2

Plasmid

#36299

Depositor

Sean Palecek

Article

Lian et al Proc Natl Acad Sci U S A. 2012 May 29.

Insert

shRNA of beta-catenin-2 (CTNNB1 Human)

Use

Lentiviral and RNAi

Tags

IRES-RFP-Puro

Expression

Mammalian

Mutation

Promoter

TetO-H1

Available since

Aug. 7, 2012

Availability

Academic Institutions and Nonprofits only
hEF1α-H2B-mOrange2-IRES-EGFP PGK-Puromycin

Plasmid

#99277

Purpose

Dual fluorescent reporter - constitutive expression of mOrange2 (549/565) localized in the nucleus, EGFP (488/507) untargeted and visualized in the cytoplasm, and Puromycin resistance

Depositor

Jordan Miller

Article

Calderon et al Biomater Sci. 2017 Jun 29. doi: 10.1039/

Inserts

H2B-mOrange2-IRES-EGFP
Puro

Use

Lentiviral

Tags

EGFP, H2B, IRES, PGK, and mOrange2

Expression

Mutation

Promoter

EF1 alpha-human and PGK-mouse

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pGL3-KRAS

Plasmid

#13919

Depositor

Frank Slack

Article

Johnson et al Cell. 2005 Mar 11. 120(5):635-47.

Insert

KRAS 3'UTR (KRAS Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

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pGL3 Zscan4 promoter

Plasmid

#69249

Purpose

Luciferase reporter for Dux4 activation of Zscan4 transcription

Depositor

Stephen Tapscott

Article

Geng et al Dev Cell. 2012 Jan 17;22(1):38-51. doi:

Insert

Zscan4 (ZSCAN4 Human)

Use

Luciferase

Tags

Expression

Mutation

The two single nucleotide mismatches found betwee…

Promoter

Zscan4

Available since

Sept. 21, 2015

Availability

Academic Institutions and Nonprofits only

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