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Plasmid#190740PurposeMammalian expression of DCX fusion to V5-tagged TurboID on microtubules (DCX as the microtubule-targeting tag)DepositorInsertdoublecortin (DCX Human)
UseTagsTurboID and V5ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1 BS (-) N protein
Plasmid#188448PurposeSARS CoV-2 N protein expressionDepositorInsertSARS-CoV-2 N protein
UseTagsStrep-TagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-SUN1 mScarlet
Plasmid#187677PurposeIn vivo visualization of SUN1DepositorInsertSUN1 (Sun1 Mouse)
UseTagsmScarletExpressionMammalianMutationWTPromoterCMVAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-SUN2 mApple
Plasmid#187679PurposeIn vivo visualization of SUN2DepositorInsertSUN2 (Sun2 Mouse)
UseTagsmAppleExpressionMammalianMutationWTPromoterCMVAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-T11HA-hMOV10-WT
Plasmid#178907Purposeexpression of WT hMOV10DepositorInsertcDNA WT hMOV10 (MOV10 Human)
UseTagsT11 beta strand of GFP-HAExpressionMammalianMutationPromoterCMV promoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-T11HA-hMOV10-CD
Plasmid#178908Purposeexpression of catalytic dead hMOV10 K530ADepositorInsertcDNA Catalytic dead hMOV10 (MOV10 Human)
UseTagsT11 beta strand of GFP-HAExpressionMammalianMutationK530A catalytic dead AAG>GCAPromoterCMV protmoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Zeo-ssIFNA2
Plasmid#183469PurposeExpression of the Salmo salar interferon ifna2DepositorInsertSalmo salar interferon alpha 2 precursor
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-β2AR-μDpTT
Plasmid#182326PurposeBeta-2 adrenergic receptor-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.DepositorInsertbeta2AR-μDronpa-TagRFP-T (ADRB2 Human)
UseTagsFull-length human beta2 adrenergic receptorExpressionMammalianMutationDronpa contains H193T mutation.PromoterCMVAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-FLAG-As-NF-kB1
Plasmid#180200PurposeExpresses FLAG-tagged Acanthoeca spectabilis NF-kB1 protein in mammalian cellsDepositorInsertAs-NF-kB1
UseTagsFLAG-tagged fusion proteinExpressionMammalianMutationaa 2-409PromoterCMVAvailable sinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Zeo-ssIFNG
Plasmid#183470PurposeExpression of the Salmo salar gamma interferonDepositorInsertSalmo salar interferon gamma precursor
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RIIα-μDpTT
Plasmid#182327PurposePKA RIIα subunit-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.DepositorInsertRIIalpha-μDronpa-TagRFP-T (PRKAR2A Human)
UseTagsFull-length human PKA type 2alpha regulatory subu…ExpressionMammalianMutationDronpa contains H193T mutation.PromoterCMVAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-H2B-μDpTT
Plasmid#182328PurposeHistone H2B-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.DepositorInsertH2B-μDronpa-TagRFP-T (H2BC3 Human)
UseTagsFull-length human histone H2BExpressionMammalianMutationDronpa contains H193T mutation.PromoterCMVAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lifeact-Dronpa
Plasmid#182330PurposeActin-targeted Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging.DepositorInsertLifeact-Dronpa
UseTagsN-terminal 17 amino acids from budding yeast Abp1…ExpressionMammalianMutationPromoterCMVAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3-hAP1 1-419
Plasmid#183644PurposeTo (ectopically) express a mutant of ATP6AP1 lacking the interacting domain with PEN2; mammalian expressionDepositorInsertATP6AP1 (ATP6AP1 Human)
UseTagsmycExpressionMammalianMutationtruncated ATP6AP1 mutant; contains only amino aci…PromoterCMVAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FluoSTEP-AKAR (C/Y)
Plasmid#181862PurposeFluoSTEP-AKAR using split CFP and cpVenus(E172) as the FRET donor and acceptor. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.DepositorInsertFluoSTEP-AKAR (C-Y)
UseTagsCFP(1-10) and cpVenus(E127)ExpressionMammalianMutationPromoterCMVAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-μDpTT
Plasmid#182321PurposePlasma membrane-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.DepositorInsertLyn-μDronpa-TagRFP-T
UseTagsN-terminal targeting sequence from Lyn kinaseExpressionMammalianMutationDronpa contains H193T mutation.PromoterCMVAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CYP450-μDpTT
Plasmid#182325PurposeEndoplasmic reticulum-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.DepositorInsertDAKAP-μDronpa-TagRFP-T
UseTagsN-terminal 27 amino acids from CYP450ExpressionMammalianMutationDronpa contains H193T mutation.PromoterCMVAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-GFP11-RhoA
Plasmid#181860PurposePart of FluoSTEP-RhoA biosensor; co-express with cpPKN-mRuby2-GFP1-10 (Addgene #181859).DepositorInsertGFP11-RhoA (RHOA Human)
UseTagsGFP11ExpressionMammalianMutationPromoterCMVAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lifeact-μDpTT
Plasmid#182322PurposeActin-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.DepositorInsertLifeact-μDronpa-TagRFP-T
UseTagsN-terminal 17 amino acids from budding yeast Abp1…ExpressionMammalianMutationDronpa contains H193T mutation.PromoterCMVAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Prom1 Del AA 6
Plasmid#184024PurposeMammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286). Deletion of amino acids 689 to 694.DepositorInsertProm1 SV8 Del AA 6 (Prom1 Mouse)
UseTagsFlagExpressionMammalianMutationDeletion of amino acids 689 to 694PromoterAvailable sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only