-
Plasmid#41156DepositorInsertLats1 (LATS1 Human)
UseTags3xMyc and PreScission SiteExpressionMammalianMutationPromoterCMVAvailable sinceJune 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro HA PIK3CA
Plasmid#12522PurposeRetroviral expression of HA tagged phosphoinositide 3-kinaseDepositorInsertPIK3CA (PIK3CA Human)
UseRetroviralTagsHAExpressionMammalianMutationPromoterAvailable sinceAug. 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMuLE Lenti Dest Neo
Plasmid#62178PurposeMuLE (Multiple Lentiviral Expression) Destination vector for use with pMuLE Entry vectors. Co-expresses Neomycin cassette under the control of a PGK promoter. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseLentiviral; Mule gateway destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
HA GSK3 beta S9A pcDNA3
Plasmid#14754PurposeMammalian expression of human GSK3 beta constitutively active mutant with HA tagDepositorInsertGSK3 beta S9A (GSK3B Human)
UseTagsHAExpressionMammalianMutationS9A. Constitutively active mutant.PromoterAvailable sinceApril 12, 2007AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGS1021
Plasmid#59575PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Twin-Strep tag and a TRP1 selection markerDepositorInsertTwin-Strep tag optimized for PCR amplification followed by TRP1 selection marker
UseE. coli cloning vectorTagsTwin-Strep tagExpressionMutationPromoterNoneAvailable sinceAug. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTet-On-TAP
Plasmid#59017PurposeConstruct allows tetracycline inducible expression of a protein of interest fused to a tandem affinity purification tag (TAP) in toxoplasma gondii.DepositorTypeEmpty backboneUseToxoplasma expressionTagsCalmodulin Binding Peptide fused with a TEV Cleav…ExpressionMutationPromoterRPS13 with Tet Operators InsertedAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
HA-HIF2α wt-pBabe-Puro
Plasmid#26055DepositorInsertHypoxia inducible factor 2 alpha (EPAS1 Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationwt cDNAPromoterAvailable sinceAug. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C3-PLK4-3xFLAG
Plasmid#69837PurposeThis plasmid encodes PLK4 isoform 1 with an N-terminal EGFP tag and C-terminal 3xFLAG tagDepositorInsertPLK4 (PLK4 Human)
UseTags3xFLAG tag and EGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET41-MBP-t-A3A-t-His
Plasmid#109231PurposeBacterial expression of A3A with N-terminal MBP an C-terminal His tag, both cleavable with TEV proteaseDepositorInserthuman APOBEC3A (APOBEC3A Optimized for E. coli expression, Human)
UseTagsHis and MBPExpressionBacterialMutationPromoterAvailable sinceOct. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-SMYD2
Plasmid#85658Purposeretroviral expression of human SMYD2DepositorInsertSMYD2 (SMYD2 Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterLTRAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMX IkB alpha M
Plasmid#12329DepositorInsertIkB alpha M (Nfkbia Mouse)
UseTagsExpressionMammalianMutationDominant-negative mutant. Many signal transductio…PromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-SMYD2 F184A
Plasmid#85659Purposeretroviral expression of human SMYD2 F184ADepositorInsertSMYD2 (SMYD2 Human)
UseRetroviralTagsFlagExpressionMammalianMutationF184APromoterLTRAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT7-7 asyn A53T
Plasmid#105727PurposeBacterial expression of mutant human alpha synucleinDepositorInsertalpha-synuclein A53T (SNCA Human)
UseTagsExpressionBacterialMutationA53TPromoterT7Available sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRcCMV myc-Plk1 wt (Nigg RG6)
Plasmid#41160DepositorInsertPlk1 (PLK1 Human)
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceMarch 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AKAR4-NES
Plasmid#64727PurposeCytosol-targeted, FRET-based PKA activity reporter.DepositorInsertAKAR4-NES
UseTagsCerulean, NES, and cpVenus[E172]ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTol2CG2-QUAS5x:GFPNLS-SV40pA
Plasmid#155122PurposeTol2 vector containing QUAS5x reporter element upstream of GFP-NLS. Includes cmlc2:mRFP-SV40pA transgenesis markerDepositorInsertQUAS5x:GFPNLS
UseZebrafish expressionTagsExpressionMutationPromoterQUAS5x-E1bAvailable sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ROR2-V5/HIS
Plasmid#205608PurposeExpression of human ROR2 receptor tyrosine kinase in mammalian cellsDepositorInsertROR2 receptor tyrosine kinase (ROR2 Human)
UseTagsV5-HisExpressionMammalianMutationPromoterCMVAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG mEmerald-LAMP1
Plasmid#168513PurposeExpression of rat LAMP1 fused with mEmerald to perform live tracking of lysosomesDepositorInsertLAMP1 (Lamp1 Rat)
UseTagsmEmeraldExpressionMammalianMutationPromoterCAGAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNAS1b split mCherry 14-3-3β ctrl
Plasmid#112031PurposeMode #2 phosphosite control (split mCherry, using 14-3-3β binding domain paired with phospho-dependent interacting peptide with TAG codon)DepositorInsert14-3-3β fused to C-terminal split mCherry, phospho-dependent binding ctrl peptide with TAG fused to N-terminal split mCherry
UseTagsSplit mCherryExpressionBacterialMutationPromoterAvailable sinceJuly 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ROS1-V5/HIS
Plasmid#205609PurposeExpression of human ROS1 receptor tyrosine kinase in mammalian cellsDepositorInsertROS1 receptor tyrosine kinase (ROS1 Human)
UseTagsV5-HisExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2023AvailabilityAcademic Institutions and Nonprofits only