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p7274 MSCV-P C-FlagHA 16 ∆DLYC

Plasmid

#163304

Purpose

Expresses HPV16 E7 ∆DLYC

Depositor

Elizabeth White

Article

Hatterschide et al Proc Natl Acad Sci U S A. 2019 Apr 2;116

Insert

HPV16 E7 ∆DLYC

Use

Retroviral

Tags

FlagHA

Expression

Mutation

∆DLYC

Promoter

MSCV LTR

Available since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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hEBI3 pEF6-Flag (MJS_003)

Plasmid

#72491

Purpose

Expression of human EBI3 in mammalian cells (Flag tag)

Depositor

Matthew Sweet

Article

Ramnath et al Immunol Cell Biol. 2015 Oct;93(9):771-9.

Insert

EBI3 (EBI3 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pCin4-3xFLAG-MKL1 T450A

Plasmid

#19843

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Aug 11. ():.

Insert

MKL1 T450A (MRTFA Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Threonine 450 to Alanine

Promoter

Available since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only
FLAG-YAP2 (8SA)-pcw107

Plasmid

#64637

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

Kris Wood, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

YAP2 (transcript variant 3) (YAP1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

8SA*

Promoter

PGK

Available since

Dec. 8, 2015

Availability

Academic Institutions and Nonprofits only
pAav-MCS-PQS1-3xFLAG

Plasmid

#84883

Purpose

rAAV-based template for genome engineering of protein C-termini containing PQS1 and 3xFLAG tags and a selection cassette

Depositor

Sven Eyckerman

Article

Vandemoortele et al Sci Rep. 2016 Jun 6;6:27220. doi: 10.103

Insert

Multicloning sites and selection cassette

Use

AAV

Tags

Expression

Mutation

Promoter

PGK

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pGSTag Flag p38 gamma

Plasmid

#20818

Depositor

Roger Davis

Article

Enslen et al J Biol Chem. 1998 Jan 16. 273(3):1741-8.

Insert

pGSTag Flag p38 gamma (Mapk12 Rat)

Use

Tags

Flag

Expression

Bacterial

Mutation

Promoter

Available since

April 14, 2009

Availability

Academic Institutions and Nonprofits only
pSLIK TT 3xFLAG LacZ neo

Plasmid

#83105

Purpose

Lentiviral vector for inducible expression of LacZ with 3xFLAG N-terminal tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

LacZ

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
Flag-MCPIP1(150-453)

Plasmid

#90109

Purpose

Mammalian expression of Flag-MCPIP1(150-453)

Depositor

Mingui Fu

Article

Liang et al J Exp Med. 2010 Dec 20;207(13):2959-73.

Insert

MCPIP1 (ZC3H12A Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pN23-LIC-His-FLAG

Plasmid

#165478

Purpose

pET based vector for protein expression in E.coli for targets fused with N-terminal Flag and His

Depositor

Renato Bruni

Article

Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

Type

Empty backbone

Use

Tags

10xHis-FLAG-TEV

Expression

Bacterial

Mutation

Promoter

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-hDIXDC1 S592A

Plasmid

#61223

Purpose

Mammalian expression vector encoding long isoform of human DIXDC1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

human DIXDC1 (DIXDC1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Changed Serine 592 to Alanine

Promoter

CMV

Available since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
pLPC-NFLAG TRF1 deltaM

Plasmid

#16064

Depositor

Titia de Lange

Article

Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

Insert

hTRF1 (TERF1 Human)

Use

Retroviral

Tags

Flag

Expression

Mutation

Deleted myb domain (aa379-439)

Promoter

Available since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only
pCMV-Flag-YAP-del 50-59

Plasmid

#33065

Depositor

Kunliang Guan

Article

Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

Insert

YAP (YAP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

deleted amino acids 50-59

Promoter

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
[pSK497] pLJC5 Flag-Depdc5(AB)

Plasmid

#109341

Purpose

Lenti-viral expression of Flag-tagged Depdc5 mutant AB

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

Insert

Depdc5 (DEPDC5 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

DIYGD(185-189)GSGSG;EQPLH(371-375)GSGSG

Promoter

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pBabepuro-flag-RalA Q72L/B

Plasmid

#11120

Depositor

Christopher Counter

Article

Lim et al Cancer Cell. 2005 Jun . 7(6):533-45.

Insert

RalA Q72A mutant, RalB C-Terminus (RALA, RALB Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Q72L, wildtype C-terminus deleted, substituted wi…

Promoter

Available since

March 8, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3-HtrA2-FLAG S142D

Plasmid

#15939

Depositor

L. Miguel Martins

Article

Plun-Favreau et al Nat Cell Biol. 2007 Sep 30. ():.

Insert

HtrA2 (HTRA2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S142D

Promoter

Available since

Nov. 5, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA FLAG TORC1 (1-630)

Plasmid

#22974

Depositor

Marc Montminy

Article

Conkright et al Mol Cell. 2003 Aug . 12(2):413-23.

Insert

TORC1 (1-630) (CRTC1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Amino acids 1-630 rather than 1-634.

Promoter

Available since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
pLJM1-Flag-Rheb-4Rs

Plasmid

#165022

Purpose

expression of Flag-Rheb-4Rs mutant protein in mammalian cells (the lysine residues on the position 109, 135, 151 and 178 on Rheb protein are mutated to arginines)

Depositor

Ken Inoki

Article

Yao et al Mol Cell. 2020 Nov 5;80(3):437-451.e6. d

Insert

Rheb-4Rs (RHEB Human)

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

change lysine residues on position 109, 135, 151,…

Promoter

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG/MKRN1(1-369)

Plasmid

#78753

Purpose

To overexpress MKRN1(1-369) in Mammalian Cells

Depositor

Jaewhan Song

Article

Lee et al EMBO J. 2009 Jul 22;28(14):2100-13. doi:

Insert

MKRN1 (MKRN1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

1-369

Promoter

CMV

Available since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV6-XL4 FLAG-TM9SF4-Fc

Plasmid

#115770

Purpose

Expression of the extracellular domain of TM9SF4 with N-terminal FLAG tag and C-terminal hIgG tag.

Depositor

Davide Comoletti

Insert

TM9SF4 (TM9SF4 Human)

Use

Tags

FLAG and hIgG/Fc

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

Oct. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3 PARL-FLAG-CT L79E

Plasmid

#13640

Depositor

Luca Pellegrini

Article

Sik et al J Biol Chem. 2004 Apr 9. 279(15):15323-9

Insert

Presenilin-associated rhomboid-like (PARL, PARL Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

changed Leu 79 to Glu

Promoter

Available since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only