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FFiBlast MSCV Puro

Plasmid

#68464

Purpose

Retroviral plasmid for establishment of blasticidin resistance Cre-reporter cell line

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Blasticidin Resistance

Use

Cre/Lox and Retroviral

Tags

Expression

Mammalian

Mutation

Inverted

Promoter

LTR

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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mEos3-caRac1

Plasmid

#92041

Purpose

Expresses mEos3 fusion with caRac1 (Q61L)

Depositor

Ji Yu

Article

Das et al Proc Natl Acad Sci U S A. 2015 Jan 20;11

Insert

RAC1 (RAC1 Human)

Use

Tags

mEos3

Expression

Bacterial, Insect, and Mamm…

Mutation

Q61L (constitutively active)

Promoter

CMV

Available since

June 8, 2017

Availability

Academic Institutions and Nonprofits only

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PH-miRFP-FRB

Plasmid

#193568

Purpose

Expresses PH domain from PLC delta1 tagged with miRFP703 and FRB mutant (T2098L) in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

PH domain from PLC delta1 (Plcd1 Rat)

Use

Tags

FRB (T2098L) and miRFP703

Expression

Mammalian

Mutation

PH domain

Promoter

CMV

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pLsCas13aGG

Plasmid

#131012

Purpose

Backbone plasmid for generating CRISPR arrays for LsCas13a. Contains a GFP-dropout cassette and a direct repeat.

Depositor

Chase Beisel

Article

Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

Inserts

direct repeat of LsCas13a
GFP expression cassette

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 27, 2019

Availability

Academic Institutions and Nonprofits only

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Halo-WIPI2B H85E

Plasmid

#175027

Purpose

Expresses WIPI2B (H85E) fused to Halo in mammalian cells to abrogate interaction with ATG16L1

Depositor

Andrea Stavoe

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2B (H85E) (WIPI2 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Histadine 85 to Glutamate (CAC to GAG)

Promoter

CMV

Available since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pFX-EGFP-KRasCT

Plasmid

#175833

Purpose

Express EGFP with plasma membrane-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

EGFP with plasma membrane-targeting sequence

Use

Tags

C-terminal hypervariable region of K-Ras 4B

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pMG343

Plasmid

#176183

Purpose

preassembled yeast MoClo level-1 backbone with toxic gene dropout cassette (ccdB and TPK2) and URA3 marker

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

TPK2+ccdB dropout cassette

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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(K) tNOS (S)_T3 (SBE120)

Plasmid

#195010

Purpose

terminator from Agrobacterium tumefaciens nopaline synthase (tNOS, MF116010) for T3 position (K/S)

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(K) tNOS (S)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

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(H) tNOS (I) _T2 (SBE119)

Plasmid

#195006

Purpose

terminator from Agrobacterium tumefaciens nopaline synthase (tNOS, MF116010) for T2 position (H/I)

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(H) tNOS (I)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

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(E) tNOS (F) _T1 (SBE118)

Plasmid

#194997

Purpose

terminator from Agrobacterium tumefaciens nopaline synthase (tNOS, MF116010) for T1 position (E/F)

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(E) tNOS (F)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

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(I) pXYL (J) P3 (SBE108)

Plasmid

#194991

Purpose

promoter of the gene xylose reductase from R. toruloides for a position P3

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(I) pXYL (J)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pmcsg7-mCRT(WT)

Plasmid

#83505

Purpose

Bacterial expression of Calreticulin

Depositor

Malini Raghavan

Article

Del Cid et al J Biol Chem. 2010 Feb 12;285(7):4520-35.

Insert

CALR (Calr Mouse)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 12, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+) neo with Smad5 CDS

Plasmid

#61800

Purpose

pcDNA3.1(+) with full length mouse Smad5 CDS

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

Smad5 CDS (Smad5 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
EGFP-GEM

Plasmid

#111191

Purpose

fluorescent fusion protein

Depositor

Johannes A. Schmid

Article

Piddini et al EMBO J. 2001 Aug 1. 20(15):4076-87.

Insert

GEM (GEM Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

T2A

Promoter

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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TAL2344

Plasmid

#36764

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

PTEN-TALEN_Left (Pten Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Expression

Mutation

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2345

Plasmid

#36765

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

PTEN-TALEN_Right (Pten Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Expression

Mutation

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pPIGA-PHD

Plasmid

#26778

Depositor

Linzhao Cheng

Article

Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

Insert

PGK-Hyg-p(A) (PGK1 )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only

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STAGR_SAMScaffold_hH1

Plasmid

#102847

Purpose

Can be used as PCR template for a STAgR reaction

Depositor

Stefan Stricker

Article

Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

Insert

STAgR Insert SAM_hH1

Use

Pcr template for stagr inserts

Tags

Expression

Mutation

Promoter

U6

Available since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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TAL2362

Plasmid

#36775

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

EZH2-TALEN_Left (Ezh2 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Expression

Mutation

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
E0030 (YFP)_CD

Plasmid

#66031

Purpose

MoClo Basic Part: CDS - Fluorescent protein. Yellow. [C:E0030:D]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

Fluorescent reporter - YFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pSYN-VWF-039

Plasmid

#120292

Purpose

VWF expression construct expressing D'D3 domains with C1099A/C1142A mutations in D3 domain as an Fc fusion protein

Depositor

Robert Peters

Article

Dong et al Blood. 2019 Jan 14. pii: blood-2018-10-8

Insert

VWF D1-D2-D'-D3-Fc

Use

Tags

Fc

Expression

Mammalian

Mutation

C1099A/C1142A

Promoter

CMV

Available since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pHJ424

Plasmid

#20682

Depositor

Stephen Shaw

Article

Hao et al J Cell Biol. 2009 Feb 9. 184(3):451-62.

Insert

Ezrin (EZR Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Changed Lys 253, 254, 262, 263 to Asn

Promoter

Available since

April 14, 2009

Availability

Academic Institutions and Nonprofits only
pGL3-RbE2Fmut

Plasmid

#32391

Depositor

Julien Sage

Article

Burkhart et al Mol Cell Biol. 2010 Apr;30(7):1729-45. E

Insert

Rb promoter (Rb1 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Mutated E2F binding site: TTTCCCGC --> TTTACATC

Promoter

Available since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
pG4-E6s-luc

Plasmid

#46324

Depositor

Steven Brown

Article

Kowalska et al Mol Cell Biol. 2012 Nov;32(22):4585-94.

Insert

5xGal4

Use

Luciferase

Tags

E boxes and luciferase

Expression

Mammalian

Mutation

five copies of a multimerized GAL4 (5×GAL4) site …

Promoter

Available since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
TAL2363

Plasmid

#36776

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

EZH2-TALEN_Right (Ezh2 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Expression

Mutation

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pSUPER-ARF1b

Plasmid

#67494

Purpose

Expression of shRNA targeting human ARF1b

Depositor

Richard Kahn

Article

Volpicelli-Daley et al Mol Biol Cell. 2005 Oct;16(10):4495-508.

Insert

HsARF1 shRNA (ARF1 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pEGB Pnos:Luciferase:Tnos (GB0112)

Plasmid

#68217

Purpose

Transcriptional unit for Firefly Luciferase Protein plant expression driven by the Nos promoter

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Luc

Use

Luciferase and Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

Pnos

Available since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
TAL2403

Plasmid

#36806

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

ATM-TALEN_Right (Atm Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Expression

Mutation

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
prNFL-Q333P-EGFP

Plasmid

#132602

Purpose

Expresses EGFP-tagged rat NFL containing the Charcot-Marie-Tooth disease type 2E Q333P mutation

Depositor

Anthony Brown

Article

Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

Insert

Nefl (Nefl Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

998A>C, 999G>C; Gln333Pro

Promoter

CMV

Available since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
L2-AA012

Plasmid

#185751

Purpose

Vector to introduce eGFP under control of pAaEF1a into the genome of the hornwort Anthoceros agrestis via Agrobacterium mediated transformation. eGFP contains a cell membrane localization tag.

Depositor

Peter Szoevenyi

Article

Frangedakis et al New Phytol. 2021 Jun 2. doi: 10.1111/nph

Inserts

HygR2
eGFP

Use

Tags

membrane localization tag Lti6b

Expression

Plant

Mutation

Promoter

Available since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
phNFL-E396K

Plasmid

#132595

Purpose

Expresses human NFL containing the Charcot-Marie-Tooth disease type 2E E396K mutation

Depositor

Anthony Brown

Article

Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

Insert

NEFL (NEFL Human)

Use

Tags

Expression

Mammalian

Mutation

1186G>A; Glu396Lys

Promoter

CMV

Available since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTK158_mCherry-4.1G-deltaCTD-hardened

Plasmid

#46360

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

Insert

4.1G (EPB41L2 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Deleted amino acids 886-1005, and modified for si…

Promoter

CMV

Available since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
CTA2-mNG211

Plasmid

#141167

Purpose

Expresses GST-SUMO tagged CTA2 Subunit with 11th Strand

Depositor

Wayne Lencer

Article

Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

Insert

SUMO-CTA2-mNG211

Use

Tags

SUMO

Expression

Bacterial

Mutation

Promoter

Available since

July 31, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmDCP1_327-372-GST-V5His6_H

Plasmid

#146503

Purpose

Insect Expression of DmDCP1_327-372

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

DmDCP1_327-372 (Dcp1 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
TAL2294

Plasmid

#36727

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

APC-TALEN_Left (APC Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Expression

Mutation

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pGK:HygroR-CMV:mKO2-C_UTR

Plasmid

#68481

Purpose

Lentivirus expressing mKO2 with UTR sensor cassette and hygromycin resistance

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

Hygromycin Resistance
mKO2-C_UTR

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

shRNA sensor cassette "B" added to 3&#x…

Promoter

CMV and pGK

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT-GFP-p150Dynactin

Plasmid

#24283

Depositor

Ron Vale

Article

Griffis et al J Cell Biol. 2007 Jun 18. 177(6):1005-15

Insert

p150 Dynactin (DCTN1-p150 Fly)

Use

Tags

GFP

Expression

Insect

Mutation

Promoter

Available since

June 1, 2010

Availability

Academic Institutions and Nonprofits only
pDGB1omega2_SF (GB0466)

Plasmid

#68243

Purpose

Twister plasmid to swap inserts from an omega1 or omega1R vector to any alpha level vector

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Solanum lycopersicum intergenic region

Use

Synthetic Biology; Unspecified

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

Oct. 27, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pDGB1alpha2_SF (GB0107)

Plasmid

#68233

Purpose

Twister plasmid to swap inserts from an alpha1 or alpha1R vector to any omega level vector

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Solanum lycopersicum intergenic region

Use

Synthetic Biology; Unspecified

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

Oct. 27, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-EF1a-V5-Vrk2

Plasmid

#120240

Purpose

Expresses V5-tagged Vrk2 in lentiviral vector

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Insert

Vrk2 (Vrk2 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

EF1a

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET_RGG-RGG_withTEVsite

Plasmid

#124940

Purpose

Expression of RGG-RGG

Depositor

Daniel Hammer, Matthew Good, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Use

Tags

His

Expression

Bacterial

Mutation

with TEV site between RGG domains

Promoter

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP_C1 I3

Plasmid

#61397

Purpose

Expresses GFP-tagged Inhibitor-3 in mammalian cells

Depositor

Hemmo Meyer

Article

Eiteneuer et al EMBO J. 2014 Oct 8. pii: e201489054.

Insert

Inhibitor-3 (PPP1R11 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 25, 2015

Availability

Academic Institutions and Nonprofits only
TAL2289

Plasmid

#36724

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

FANCG-TALEN_Right (Fancg Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Expression

Mutation

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pAsCpf1GG

Plasmid

#131011

Purpose

Backbone plasmid for generating CRISPR arrays for AsCas12a. Contains a GFP-dropout cassette and a direct repeat.

Depositor

Chase Beisel

Article

Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

Inserts

direct repeat of AsCas12a
GFP expression cassette

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET_RGG-RFP-RGG

Plasmid

#124944

Purpose

Exoression of RGG-RFP-RGG

Depositor

Daniel Hammer, Matthew Good, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RGG-RFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Use

Tags

His and mCherry

Expression

Bacterial

Mutation

Promoter

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pOpLexA::mini35s (GB0733)

Plasmid

#68180

Purpose

Provides the 4xLexA Operator fused to CaMV 35s minimal promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

4xOpLexA:mini35S

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV5-Fyn C3,6S

Plasmid

#19979

Depositor

Marilyn Resh

Article

Alland et al J Biol Chem. 1994 Jun 17. 269(24):16701-

Insert

Fyn (FYN Human)

Use

Tags

Expression

Mammalian

Mutation

C3,6S

Promoter

Available since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only
TAL2272

Plasmid

#36709

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

KRAS-TALEN_Left (Kras Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Expression

Mutation

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2273

Plasmid

#36710

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

KRAS-TALEN_Right (Kras Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Expression

Mutation

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
YFP-Gamma 5

Plasmid

#36044

Depositor

Narasimhan Gautam

Article

Akgoz et al Cell Signal. 2006 Oct;18(10):1758-68. Ep

Insert

G protein subunit Gamma 5 (Gng5 Rat)

Use

Tags

His and YFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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