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Plasmid#85212PurposeLentiviral vector expressing mOrange2 fluorescent protein driven by SFFV promoterDepositorInsertmOrange2
UseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceJan. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLEX-EF1a ANXA11-mEmerald
Plasmid#164210PurposepLEX lentivirus backbone expresses mEmerald tagged ANXA11 under EF1a promoterDepositorInsertANXA11 (ANXA11 Human)
UseLentiviralTagsmEmeraldExpressionMutationPromoterEF1aAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
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HP17_KO7
Plasmid#120346PurposeHelper plasmid for production of phagemid ssDNA, based on M13KO7DepositorInsertCoding part of the M13KO7 helper phage genome
UseTagsExpressionBacterialMutationPromoternative phage promotersAvailable sinceAug. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
Rab5a-pmCherryC1
Plasmid#27679DepositorInsertRab5a (Rab5a Mouse)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceMay 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
CFP-FKBP (CF)
Plasmid#20160DepositorInsertCFP-FKBP (FKBP1A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSLQ11353
Plasmid#183098PurposepHR-CAG-sfGFP* ABE_3-WPREDepositorInsertGFP*
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pZR158_Lenti-U6-gRNA-10xCS1-PP7_hPGK-PCP-P65-HSF1-Puro
Plasmid#180273PurposeLentiviral expression vector for CRISPRa-SAM sgRNA with 10x capture sequence, and PCP-P65-HSF1 cassetteDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFX-EGFP-Rab5
Plasmid#174454PurposeExpress EGFP with early endosome-targeting sequence in mammalian cellsDepositorInsertEGFP with early endosome-targeting sequence
UseTagssmall GTPase (Rab5)ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
VN-gamma Synuclein
Plasmid#89733Purposeexpresses human gamma Synuclein fused to the N-terminal part of VenusDepositorInsertSNCG (SNCG Human)
UseTagsN-terminal part of VenusExpressionMammalianMutationPromoterCMVAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hA3B-BE3
Plasmid#113411PurposeExpresses hA3B-BE3 in mammalian cellsDepositorInserthA3B-BE3 (APOBEC3B Human, S. pyogenes and Bacteriophage PBS2)
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLentiRNACRISPR_007 - TetO-NLS-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast
Plasmid#138149PurposeExpresses RfxCas13d in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleusDepositorInsertCasRx
UseLentiviralTagsNLS-HAExpressionMutationPromoterAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EML4-ALK variant 3a G1202R
Plasmid#183830PurposeExpress EML4-ALK fusion variant 3a (E6a;A20) harboring ALK G1202R mutation in mammalian cellsDepositorUseLentiviralTagsThe fusion protein of EML4 exon 1-6a and ALK exon…ExpressionMammalianMutationG1202RPromoterCMVAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCA24N-ligase
Plasmid#87741PurposeIPTG-inducible expression of T4 DNA ligase for protein purificationDepositorInsertT4 DNA ligase (30 )
UseTags6xHisExpressionBacterialMutationPromoterT5-lacAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCFD4-U6:1_U6:3tandemgRNAs
Plasmid#49411Purposeexpression of two gRNAs from Drosophila U6:1 and U6:3DepositorInsertsdU6-1:gRNA
dU6-3:gRNA
UseCRISPRTagsExpressionInsectMutationPromoterdU6-1 and dU6-3Available sinceJan. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
BE4
Plasmid#100802PurposeExpresses BE4 in mammalian cellsDepositorInsertBE
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_KEAP1_WT
Plasmid#81925PurposeGateway Donor vector containing KEAP1, part of the Target Accelerator Plasmid Collection.DepositorInsertKEAP1 (KEAP1 Human)
UseGateway entry vectorTagsExpressionMutationC624SPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDAC627
Plasmid#195240PurposeMammalian expression of Csm complex from separate promoters; Puro backbone (Used for RNA knockdown)DepositorInsertFLAG-NLS-Csm1; FLAG-NLS-Csm2; FLAG-NLS-Csm3; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; Puro
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP_TOMM20_sfCherry2(11)
Plasmid#83033PurposeExpresses sfCherry2(11) tagged TOMM20 (human) in mammalian cells cellsDepositorInsertTOMM20_sfCherry+11
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
PLKO.1-Scrambled
Plasmid#136035PurposeScrambled shRNA (negative control) inserted into the PLKO.1 plasmid (CCTAAGGTTAAGTCGCCCTCG)DepositorInsertNone (Scrambled)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHelper_ShCAST_sgRNA
Plasmid#127921PurposeExpresses ShCAST and an empty sgRNA scaffold. New targets can be added using Golden Gate assembly (LguI sites).DepositorInsertShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning (LguI sites)
UseTagsExpressionMutationPromoterAvailable sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDonor_ShCAST_kanR
Plasmid#127924PurposeKanamycin resistance donor for ShCASTDepositorInsertKanR, R6K origin of replication
UseTagsExpressionMutationPromoterAvailable sinceAug. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
CRY-Gal∆DD (B1013)
Plasmid#92035PurposeExpresses CRY2 (full-length, with endogenous NLS) fused to Gal4 (aa1-65 of Gal4BD), downstream of mCherry-IRESDepositorInsertCRY2 (CRY2 Mustard Weed)
UseTagsExpressionMammalianMutationFusion of plant CRY2 to Gal4 binding domain (AA1-…PromoterAvailable sinceJuly 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
Flag-miniTurbo
Plasmid#124647PurposeExpresses Flag-miniTurbo fusion for proximity labelingDepositorTypeEmpty backboneUseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceApril 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXPR_219
Plasmid#164559PurposeLentiviral vector that encodes two sgRNA expression cassettes. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.DepositorInsertsFirst sgRNA cassette
Second sgRNA cassette
UseCRISPR and Lentiviral; Dual sgrnaTagsExpressionMammalianMutationPromoterHuman U6 and Mouse U6Available sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hA3A-BE3
Plasmid#113410PurposeExpresses hA3A-BE3 in mammalian cellsDepositorInserthA3A-BE3 (APOBEC3A Human, S. pyogenes and Bacteriophage PBS2)
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-DUX4-CA
Plasmid#99281PurposeAll-in-one lentivector with tet-inducible DUX4 (codon sequences altered)DepositorInsertDUX4 (codon altered) (DUX4 Human)
UseLentiviralTagsExpressionMammalianMutationcodon alteredPromoterTREAvailable sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Sport6-CD9-pHluorin
Plasmid#130905PurposeExpression of CD9-pHluorin for visualization of multivesicular body-plasma membrane fusion.DepositorInsertCD9-pHluorin (CD9 Human, Aequorea victoria)
UseTagspHluorinExpressionMammalianMutationphluorin inserted between Asn-50 and Asn-51 in ex…PromoterCMVAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pHR_TRE3G-p300-dCas9-P2A-mCherry
Plasmid#138456Purpose2nd Generation Lentiviral vector. Expresses an N-terminal p300-dCas9 fusion protein and mCherryDepositorInsertp300 core
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCarmine in pcDNA3
Plasmid#109486Purposemammalian expression vector for mCarmine, a far red emitting fluorescent protein (excitation: 603; emission: 675)DepositorInsertmCarmine
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR_TRE3GS-->sGFP-P2A-BFP
Plasmid#162234PurposeLentiviral vector for the expression of TRE3GS-->sGFP-P2A-BFP in mammalian cellsDepositorInsertEGFP and BFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-EFS-SaABE8eV106W-bGH-U6-sgRNA-BsmBI
Plasmid#189924PurposeAAV genome encoding SaABE8eV106W and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installationDepositorInsertSaABE8e V106W
UseAAVTagsExpressionMutationSaCas9 D10A, TadA ABE8e V106WPromoterEFSAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Phi3-AURKB-PGK-mCherry
Plasmid#153316PurposeExpresses AURKB and mCherry in mammalian cells.DepositorInsertAURKB (AURKB Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-GSK3β-#2
Plasmid#32497DepositorInsertGSK3B (GSK3B Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6Available sinceSept. 26, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FLAG-APEX2-OPTN
Plasmid#175760PurposeLentiviral vector expressing N-terminally tagged APEX2-FLAG-OPTNDepositorInsertOPTN (OPTN Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pArcCreER
Plasmid#46389DepositorInsertsUseMouse TargetingTagsExpressionMutationPromoterAvailable sinceJuly 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pB_tetO7_sfGFP
Plasmid#196616PurposePlasmid for integrating tetO7-controlled sfGFP into the HO region in yeastDepositorInsertSuperfolder GFP
UseTagsExpressionYeastMutationPromotertetO7 promoterAvailable sinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLentiCas9-P2A-BFP
Plasmid#149447PurposeExpresses Cas9 and BFP in a lentiviral vector.DepositorInsertP2A-BFP
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterIn frame with Cas9Available sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
Frb-NLS-mCherry
Plasmid#217613Purposepart B of SPARK-OFFDepositorInsertFrb-NLS-mCherry
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only