Addgene

We narrowed to 6,242 results for: sms

Showing: 3401 - 3420 of 6242 results

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GfaABC1D BARK D110A p2A mCherry

Plasmid

#117692

Purpose

GfaABC1D-iBARK(D110A)-p2A-mCherry: Negative control viral expression vector for astrocyte Gaq silencing

Depositor

Baljit Khakh

Article

Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

Insert

BARKrgs D110A peptide

Use

AAV

Tags

HA / FLAG

Expression

Mutation

D110A

Promoter

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pBABEneo-HRASV12

Plasmid

#71304

Purpose

Retroviral expression of H-RAS-V12

Depositor

Jayanta Debnath

Article

Lock et al Mol Biol Cell. 2011 Jan 15;22(2):165-78.

Insert

H-RAS-V12 (HRAS Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

G12V mutation

Promoter

Available since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR_zeo_sgTP53

Plasmid

#183180

Purpose

pLentiCRISPR that is selectable with zeocin with an sgRNA targeting TP53

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

sgTP53 (TP53 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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PKT2/CLP-AKT

Plasmid

#20281

Depositor

John Ohlfest

Article

Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

Insert

AKT (AKT1 Human)

Use

Transposon

Tags

Expression

Mutation

Promoter

Available since

Dec. 3, 2009

Availability

Academic Institutions and Nonprofits only

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pVL1393-MAL-SYK

Plasmid

#157990

Purpose

Expresses SYK in insect cell (sf9)

Depositor

Byung Il Lee

Article

Lee et al Mol Cells. 2018 Jun;41(6):545-552. doi:

Insert

Spleen tyrosine kinase (SYK Human)

Use

Tags

Met-Ala-Leu and add Leu,Glu residues +His tag

Expression

Insect

Mutation

Promoter

Available since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pXL002-ishRNA-beta-catenin-1

Plasmid

#36297

Depositor

Sean Palecek

Article

Lian et al Proc Natl Acad Sci U S A. 2012 May 29.

Insert

shRNA of beta-catenin-1 (CTNNB1 Human)

Use

Lentiviral and RNAi

Tags

IRES-RFP-Puro

Expression

Mammalian

Mutation

Promoter

TetO-H1

Available since

Aug. 7, 2012

Availability

Academic Institutions and Nonprofits only
pET30a-SARS-CoV2-NP

Plasmid

#165090

Purpose

protein expression in E. coli

Depositor

Rick Tarleton

Article

Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

Insert

Severe acute respiratory syndrome coronavirus 2 nucleocapsid protein N-terminal (N SARS-CoV2)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 10, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pSBbi-FoxO1_1R_10A_3D

Plasmid

#106278

Purpose

Fluorescent fusion protein for FoxO1

Depositor

Laura Heiser

Article

Gross et al Cell Syst. 2019 Dec 18;9(6):580-588.e4.

Inserts

Foxo1a (Foxo1 Mouse)
NLS-mCherry-NLS-P2A-Puromycin

Use

Tags

Clover fluorescent protein

Expression

Mammalian

Mutation

Deleted amino acids 401-636, S209A, H212R, S215A,…

Promoter

EF1a/RPBSA

Available since

July 27, 2018

Availability

Academic Institutions and Nonprofits only

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AttB_ACE2-2A-TMPRSS2_IRES_iRFP670-H2A-P2A-PuroR

Plasmid

#190077

Purpose

express human ACE2 and human TMPRSS2 with selectable markers iRFP670 and puromycin resistance gene

Depositor

Kenneth Matreyek

Article

Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

Insert

ACE2 (ACE2, TMPRSS2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

none

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-MT mouse Axin (Axin MTFu1)

Plasmid

#21287

Depositor

Frank Costantini

Article

Zeng et al Cell. 1997 Jul 11. 90(1):181-92.

Insert

Axin (Axin1 Mouse)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 15, 2009

Availability

Academic Institutions and Nonprofits only

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AAV8-hSyn-flex-miR30-eGFP-shDyn

Plasmid

#132716

Purpose

Encodes Cre-dependent short hairpin RNA (shRNA) that targets the 3’-untranslated region of the rat Pdyn gene, which encodes dynorphin

Depositor

Robert Messing

Article

Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

Insert

GFP-shDyn (Pdyn Rat)

Use

AAV

Tags

Expression

Mutation

Promoter

Synapsin 1

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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MSI2-ADA

Plasmid

#166967

Purpose

Express the fusion protein of human MSI2 and the hyperactive catalytic domain of Drosophila ADAR

Depositor

Michael Kharas

Article

Nguyen et al Nat Commun. 2020 Apr 24;11(1):2026. doi:

Insert

MSI2-ADA

Use

Retroviral

Tags

MSI2-ADA

Expression

Mammalian

Mutation

Promoter

MSCV

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-UAP56

Plasmid

#157661

Purpose

Expresses UAP56 in E.coli cell

Depositor

Byung Il Lee

Article

Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

Insert

Spliceosome RNA helicase DDX39B (DDX39B Human)

Use

Tags

GST tag

Expression

Bacterial

Mutation

deleted amino acids 1-43

Promoter

Available since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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HyperADAR control

Plasmid

#166969

Purpose

Express the Hyperactive catalytic domain of Drosophila ADAR and linker at the 5' end of this domain, in the MSCV-IRES-GFP

Depositor

Michael Kharas

Article

Nguyen et al Nat Commun. 2020 Apr 24;11(1):2026. doi:

Insert

linker-hyperADAR

Use

Retroviral

Tags

linker-HyperADAR

Expression

Mammalian

Mutation

Promoter

MSCV

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pOPINK-OTUD1 (full-length, aa 1-481)

Plasmid

#61405

Purpose

Expresses human OTUD1 (full-length) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD1 (OTUD1 Human)

Use

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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CAG FLEX BARK D110A p2A mCherry

Plasmid

#117694

Purpose

CAG-FLEx-iBARK(D110A)-p2A-mCherry: Negative control viral expression vector for Cre-dependent Gaq silencing

Depositor

Baljit Khakh

Article

Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

Insert

BARKrgs D110A peptide

Use

Cre/Lox

Tags

HA / FLAG

Expression

Mutation

D110A

Promoter

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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BAP1 C91A

Plasmid

#81025

Purpose

Retroviral vector containing human BAP1 C91A (catalytically inactive mutant) and Thy1.1 selection marker

Depositor

Anjana Rao

Article

Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

Insert

BAP1 (BAP1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

BAP1 C91A (point mutation, catalytically inactive)

Promoter

5’LTR

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only
pGL3-E-cadherin promoter

Plasmid

#61798

Purpose

Luciferase reporter containing the mouse E-cadherin promoter

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

-178/+92 fragment of the mouse Cdh1 promoter (Cdh1 Mouse)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pLV-mScarlet-SARS-CoV-2-5'UTR

Plasmid

#184638

Purpose

Fluorescent reporter for mammalian cells: Expression of mScarlet with SARS-CoV-2 5'UTR

Depositor

Hao Wu

Article

Vora et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

mScarlet

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 2, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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HA-mDyn1 pcDNA3

Plasmid

#36263

Depositor

Pietro De Camilli

Article

Ferguson et al Science. 2007 Apr 27;316(5824):570-4.

Insert

Dynamin 1 (Dnm1 Mouse)

Use

Tags

HA

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

April 20, 2012

Availability

Academic Institutions and Nonprofits only