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Plasmid#73218PurposeBacterial expression vector for Z1907 affibody protein (A) with N-terminal His10, GST and HRV3C cleavage site (cysteine tag)DepositorInsert10XHis-GST-HRV-dHer1cys
UseTags10XHis-GST-HRV and The dimer of the Her1 Affibody…ExpressionBacterialMutationPromoterT7Available sinceMarch 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
HOXA2 (murine) HIS-tag pET
Plasmid#8557DepositorInsertHOXA2 (Hoxa2 Mouse)
UseTagsHis and T7ExpressionBacterialMutationcontains 5 additional amino acids from 5' un…PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
BPV00605 pET21GG2-His(6)-BECN(1-265)
Plasmid#136686PurposeExpresses the Beclin-1 IDR+CC domains (a.a. 1-265) with a N-terminal hexa-Histidine tagDepositorInsertBeclin-1 IDR + CC (BECN1 Human)
UseTagsHexa-HistadineExpressionBacterialMutationDNA optimized for E. coli expressionPromoterT7Available sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
HOXA10 (human) HIS-tag pET
Plasmid#8516DepositorInsertHOXA10 (HOXA10 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
PET-B-SpCas9-HF1-NLS-6xHis
Plasmid#207385PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertB-SpCas9-HF1
UseCRISPRTags6xHisExpressionBacterialMutationN497A, R661A, Q695A, Q926A, amino acids 1005-1013…PromoterT7Available sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
HOXA5 (human) HIS-tag pET
Plasmid#8562DepositorInsertHOXA5 (HOXA5 Human)
UseTagsHis and T7ExpressionBacterialMutationSacI site at amino acids 1-2 was used to clone in…PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET11-His6-Pif6-sfGFP-ssrA
Plasmid#140891PurposeExpression of PIF-sfGFP-ssrA in E. coliDepositorInsertPIF-sfGFP-ssrA
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)-NADPH-LUCID-1 (C50 5.1 nM)
Plasmid#117908PurposePlasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 5.1 nMDepositorInsertSNAP_Pro30_[cpNLuc23_24]eDHFR
UseTagsHis-tag and Strep-tagExpressionBacterialMutationPromoterT7Available sinceOct. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
human KANK1 Ankyrin Repeats pET151
Plasmid#164053PurposeExpresses human KANK1 Ank domain in bacterial cellsDepositorInsertKidney Ankyrin Repeat-Containing Protein 1 (KANK1 Human)
UseTagsHis-tag, TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-GST-TNRC6A-(935-984)
Plasmid#42050DepositorInsertTNRC6A (TNRC6A Human)
UseTagsGST and HisExpressionBacterialMutationcontain aa935-984PromoterAvailable sinceMarch 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
HOXA1 (murine) HIS-tag pET
Plasmid#8556DepositorInsertHOXA1 (Hoxa1 Mouse)
UseTagsHis and T7ExpressionBacterialMutationclone contains 130 AA including the homeodomain, …PromoterAvailable sinceJuly 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbr-HIS-ddFLN4-Fgß
Plasmid#101719Purposewildtype Fgß peptide with ddFLN4 fingerprint domain with N-terminal ybbr tag for covalent immobilization - resulting in weak forcesDepositorInsertddFLN4 fingerprint, Fgß peptide (FGB Human, Dictyostelium discoideum)
UseTagsHIS and ybbrExpressionBacterialMutationC18S in ddFLN4PromoterT7Available sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-28a-His6-SUMO-VAMP2
Plasmid#135551PurposeExpress mouse VAMP2 in E. coli BL21, purified via HisPur Ni-NTA ResinDepositorInsertVAMP2
UseTags6xHis tag, thrombin recognition and cleavage site…ExpressionBacterialMutationNo ATG start codonPromoterT7 promoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
HOXA11 (murine) HIS-tag pET
Plasmid#8517DepositorInsertHOXA11 (Hoxa11 Mouse)
UseTagsT7ExpressionBacterialMutationmakes protein by WesternPromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pF711-pET28a-Hs-delta25-MCAD-NHis wt
Plasmid#85112Purposeexpression of recombinant human mature (delta25) MCAD wt with N-terminal 6xHis-tagDepositorInserthuman mature (delta25) MCAD (ACADM Human)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
HOXB2 (human) HIS-tag pET
Plasmid#8522DepositorInsertHOXB2 (HOXB2 Human)
UseTagsHis and T7ExpressionBacterialMutationStarts at AA #2; makes protein by WesternPromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET-dSpCas9-HF1-VP64-6xHis
Plasmid#92118PurposeExpression of dead/inactive increased fidelity SpCas9-HF1-VP64-6xHis in bacterial cellsDepositorInsertdead/inactive SpCas9-HF1-NLS-3xFLAG-VP64
UseCRISPRTags3xFLAG, 6xHis, NLS, and VP64ExpressionBacterialMutationD10A, N497A, R661A, Q695A, H840A, Q926APromoterT7Available sinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-3a-His-MBP-TEV-VC_Cas1_WT
Plasmid#120364PurposeExpresses wildtype V-C Cas1DepositorInsertVC_Cas1
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceApril 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-3a-His-MBP-TEV-VD_Cas1_WT
Plasmid#120365PurposeExpresses wildtype V-D Cas1DepositorInsertVD_Cas1
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceApril 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pF708-pET28a-Hs-delta38-METTL20-NHis wt
Plasmid#85109Purposeexpression of recombinant human mature (delta38) METTL20 wt with N-terminal 6xHis-tagDepositorInserthuman mature (delta38) METTL20 (METTL20 Human)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only