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Plasmid#214295PurposeExpress split-Rac1 fragment fused with FKBP and MBPDepositorInsertSplit-Rac1 fragment fused with FKBP (RAC1 Human)
UseTagsMaltose-binding protein (MBP)ExpressionBacterialMutationRac1-Q61LPromoterT7Available sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21b-RhoA/13C-FKBP-MBP
Plasmid#214299PurposeExpress split-RhoA fragment fused with FKBP and MBPDepositorInsertSplit-RhoA fragment fused with FKBP (RHOA Human)
UseTagsMaltose-binding protein (MBP)ExpressionBacterialMutationRhoA-Q63LPromoterT7Available sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21b-KRas/13C-FKBP-MBP
Plasmid#214303PurposeExpress split-KRas fragment fused with FKBP and MBPDepositorInsertSplit-KRas fragment fused with FKBP (KRAS Human)
UseTagsMaltose-binding protein (MBP)ExpressionBacterialMutationKRas-Q61LPromoterT7Available sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mScarlet-TEV-Strep-His
Plasmid#210823PurposeBacterial overexpression vector for C-terminally Strep and His tagged mScarlet fluorescent proteinDepositorInsertmScarlet
UseTagsTwinStrep, 10xHisExpressionBacterialMutationPromoterT7Available sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mTagBFP2-TEV-Strep-His
Plasmid#210824PurposeBacterial overexpression vector for C-terminally Strep and His tagged mTagBFP2 fluorescent proteinDepositorInsertmTagBFP2
UseTagsTwinStrep, 10xHisExpressionBacterialMutationPromoterT7Available sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-GAL4-PARP1-CAT-L713F
Plasmid#175949PurposeBacterial expression of a fusion of yeast GAL4 DNA binding domain and human PARP1 CAT domain containing L713F gain-of-function mutationDepositorInsertGAL4 DNA binding domain (GAL4 Budding Yeast)
UseTags6xHis tagExpressionBacterialMutationL713F, V762A for the 'PARP1-CAT-L713F' …PromoterT7Available sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21b-effector-ncRNA-rrt-His8
Plasmid#194089PurposeExpresses Ec86 operon (effector, ncRNA and reverse transcriptase) in Escherichia coliDepositorInsertEc86 effector-ncRNA-rrt
UseTagsHis8ExpressionMutationPromoterT7Available sinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-H12TEV-hEIF5B-587~1220
Plasmid#208637Purposefor E. coli expression truncated human eIF5B (ENST00000289371), aa587~1220DepositorInserthuman eIF5B (ENST00000289371) aa 587~1220 (EIF5B Human)
UseTags6xhisExpressionBacterialMutationcontain SNP rs1376443, protein sequence not chang…PromoterT7Available sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3-1~41-mEGFP(A206K)-TwStrep
Plasmid#208628Purposefor E. coli expression of truncated human RPL3 (ENST00000216146), aa1~41, fused with mEGFP(A206K) and Twin Strep tagDepositorInserthuman RPL3 (ENST00000216146) aa 1~41 (RPL3 Human)
UseTags6xHis, Twin Strep, and mEGFP(A206K)ExpressionBacterialMutationonly aa 1~41, other residues not includedPromoterT7Available sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-29b(+)_His-CBD-EmEndoF3
Plasmid#218194PurposeBacterial expression of a His-tagged Chitin Binding fusion of Elizabethkingia miricola EndoF3DepositorInsertEndoF3
UseTagstufA-His-CBDExpressionBacterialMutationcodon optimized Ecoli, residues 41-329PromoterT7Available sinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-29b(+)_His-CBD-EmEndoF1
Plasmid#218195PurposeBacterial expression of a His-tagged Chitin Binding fusion of Elizabethkingia miricola EndoF1DepositorInsertEndoF1
UseTagstufA-His-CBDExpressionBacterialMutationcodon optimized Ecoli, residues 51-339PromoterT7Available sinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-syt1 (96-421) I367C
Plasmid#213620PurposeBacterial expression of SUMO-tagged rat syt1 (96-421) I367CDepositorInsertsyt1 (96-421) I367C (Syt1 Rat)
UseTagsSUMOExpressionBacterialMutationC277A; I367CPromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-syt7 (136-403)
Plasmid#213621PurposeBacterial expression of SUMO-tagged mouse syt7 (136-403)DepositorInsertsyt7 (136-403) (Syt7 Mouse)
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-Doc2β C2AB (G361C)
Plasmid#213631PurposeBacterial expression of SUMO-tagged rat Doc2β C2AB (G361C)DepositorInsertDoc2β C2AB (G361C) (Doc2b Rat)
UseTagsSUMOExpressionBacterialMutationG361C, all original cysteines mutated to alaninesPromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-cPLA2 C2 domain (Y96C)
Plasmid#213633PurposeBacterial expression of SUMO-tagged human cPLA2 C2 domain (Y96C)DepositorInsertcPLA2 C2 domain (Y96C) (PLA2G4A Human)
UseTagsSUMOExpressionBacterialMutationY96C, C139A, C141SPromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a_mouse-Pro-IL-18_humanized-triple-mutant
Plasmid#214339PurposeProtein overexpression in bacteriaDepositorInsertIL18 (Il18 Mouse)
UseTagsHis-TEVExpressionBacterialMutationENGD29DDEN_N36Y_H41EPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET24a-His6-CUbo(K63R)-MCS
Plasmid#212727PurposeBacterial expression of a protein of interest with N-terminal His6-CUbo(K63R)-fusion, contains multiple cloning siteDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET24a-His6-CUbo(K48R)-MCS
Plasmid#212726PurposeBacterial expression of a protein of interest with N-terminal His6-CUbo(K48R)-fusion, contains multiple cloning siteDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET15b-His6-CUbo(K63R)-GFP
Plasmid#212722PurposeBacterial expression of His6-CUbo(K63R)-GFPDepositorInsertGFP
UseTags6xHis-CUbo(K63R)ExpressionBacterialMutationPromoterAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET15b-His6-CUbo(K48R)-GFP
Plasmid#212721PurposeBacterial expression of His6-CUbo(K48R)-GFPDepositorInsertGFP
UseTags6xHis-CUbo(K48R)ExpressionBacterialMutationPromoterAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only