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Plasmid#63794PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eVenus Twin-Strep tag followed by URA3 selection markerDepositorInserteVenus-Twin-Strep tag followed by URA3
UseE. coli cloning vectorTagseVenus-Twin-StrepExpressionMutationPromoternoneAvailable sinceApril 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGS1422
Plasmid#63793PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eCitrine Twin-Strep tag followed by URA3 selection markerDepositorInserteCitrine-Twin-tag followed by URA3 selection marker
UseE. coli cloning vectorTagseCitrine-Twin-tagExpressionMutationPromoternoneAvailable sinceApril 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGS1512
Plasmid#63788PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eVenus Strep-tag II followed by URA3 selection markerDepositorInserteVenus-Strep-tag II followed by URA3
UseE. coli cloning vectorTagseVenus-Strep-tag IIExpressionMutationPromoternoneAvailable sinceApril 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGS1312
Plasmid#63786PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eCFP Strep-tag II followed by URA3 selection markerDepositorInserteCFP-Strep-tag II followed by URA3
UseE. coli cloning vectorTagseCFP-Strep-tag IIExpressionMutationPromoternoneAvailable sinceApril 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBacPak-FLAG-HIS-Sgf11
Plasmid#52287PurposeExpresses Drosophila Sgf11DepositorInsertSgf11 (Sgf11 Fly)
UseTagsHIS, FLAGExpressionInsectMutationPromoterAvailable sinceMay 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBacPak-HIS-FLAG-CG9866
Plasmid#52290PurposeExpresses Drosophila Ataxin-7DepositorInsertAtaxin-7 (CG9866 Fly)
UseTagsHIS FLAGExpressionInsectMutationPromoterAvailable sinceApril 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET30-GBF-E6N
Plasmid#21699DepositorInsertProtein G domain B1 fused N-terminal zinc binding domain of human papillomavirus type 16 (residues 2-74) (E6 Human papillomavirus type 16)
UseTagsProtein G B1 domainExpressionBacterialMutationGB1 (1-56aa) fusion tag with mutations at three p…PromoterAvailable sinceJuly 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PKAsub-YPet-Kras
Plasmid#138206PurposeEncodes C-terminal (substrate) fragment of FRET-based bimolecular PKA activity reporter (bimAKAR); plasma membrane targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.DepositorInsertPKAsub-YPet-Kras
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pHCMM1
Plasmid#167452PurposeExpresses C.elegans PUP-2 linked to RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (+PAB)] from the TEF1 promoterDepositorInsertPTEF1_yeGFP_scPAB14RRMS_cePUP-2_URA3_TADH1
UseTagsExpressionYeastMutationPromoterTEF1Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pHCMM2
Plasmid#167453PurposeExpresses C.elegans PUP-2 linked to RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (+PAB)] from the Sec63 promoterDepositorInsertPSEC63_yeGFP_scPAB14RRMS_cePUP-2_URA3_TADH1
UseTagsExpressionYeastMutationPromoterPSEC63Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pHCMM3
Plasmid#167454PurposeA control chimera; expresses C. elegans PUP-2 from the Sec63 promoter without the RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (-PAB)]DepositorInsertPSEC63_yeGFP_cePUP-2_URA3_TADH1
UseTagsExpressionYeastMutationPromoterPSEC63Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-PDGFRA-V5/HIS
Plasmid#201987Purposeexpression of human PDGFRA receptor tyrosine kinase in mammalian cellsDepositorInsertPDGFRA receptor tyrosine kinase (PDGFRA Human)
UseTagsV5, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
KCC2B_HUMAN_D0
Plasmid#79718PurposeThis plasmid encodes the kinase domain of KCC2B. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertKCC2B
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-GSK3β-#2
Plasmid#32497DepositorInsertGSK3B (GSK3B Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6Available sinceSept. 26, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-GSK3β-#1
Plasmid#32496DepositorInsertGSK3B (GSK3B Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6Available sinceSept. 26, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mEGFP-CaMKIIa
Plasmid#127389PurposeFluorescent reporter for Ca2+/calmodulin-dependent protein kinase II alphaDepositorInsertcalcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
UseTagsmEGFPExpressionMammalianMutationPromoterpCAGAvailable sinceNov. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DO_DIO-TdTomato_EGFP-WPRE-pA
Plasmid#37120PurposeDO/DIO Cre-Switch. Expresses tdTomato in Cre negative cells, expresses EGFP in Cre positive cellsDepositorInsertTdTomato-EGFP
UseAAV and Cre/Lox; Cre-switchTagsExpressionMutationPromoterEF1aAvailable sinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
mCherry-NES-SspB-LARG-DH-P2A-iLIDcaax
Plasmid#173870PurposeMammalian expression of mCherry-NES-SspB-LARG-DH-P2A-iLIDcaax (opto-RhoA)DepositorInsertmCherry-NES-SspB-LARG-DH-P2A-iLIDcaax
UseTagsC-terminal CAAX-box and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCherry-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax
Plasmid#173872PurposeMammalian expression of mCherry-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax (opto-Cdc42)DepositorInsertmCherry-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax
UseTagsC-terminal CAAX-box and mCherryExpressionMammalianMutationPromoterAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCherry-NES-SspB-MCS-P2A-iLIDcaax
Plasmid#173869PurposeMammalian expression of mCherry-NES-SspB-P2A-iLIDcaax (opto-control) containing multiple cloning sites (MCS)DepositorInsertmCherry-NES-SspB-MCS-P2A-iLIDcaax
UseTagsC-terminal CAAX-box and mCherryExpressionMammalianMutationPromoterAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only