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Plasmid#110216PurposeExpression in mammalian cells of of full-length SPRTN protein carrying Y117C mutation, a catalytically-impaired mutant, and Flag-tag at N-terminus.DepositorInsertSPRTN (SPRTN Human)
UseTagsFlagExpressionMammalianMutationY117C, P296L (see depositor comments below)PromoterAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV 3xFLAG-LC3A G120
Plasmid#123091PurposeExpresses 3xFLAG-LC3A G120 in mammalian cells.DepositorInsertMicrotubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)
UseTags3xFLAGExpressionMammalianMutationDeleted amino acid 121. Stop codon after G120.PromoterCMVAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRK5 Flag-RagC(L91P)
Plasmid#99726PurposeMammalian expression of Flag-RagC(L91P)DepositorInsertRagC (RRAGC )
UseTagsFlagExpressionMutationL91PPromoterCMVAvailable sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRK5 Flag-RagC(K84T)
Plasmid#99727PurposeMammalian expression of Flag-RagC(K84T)DepositorInsertRagC (RRAGC )
UseTagsFlagExpressionMutationK84TPromoterCMVAvailable sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRK5 Flag-RagA(R37P)
Plasmid#99717PurposeMammalian expression of Flag-RagA(R37P)DepositorInsertRagA (RRAGA )
UseTagsFlagExpressionMutationR37PPromoterCMVAvailable sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-C-8His-Flag
Plasmid#157678PurposeC-terminal tagging vectorDepositorInsert8His-Flag
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTXB3-Flag PLK1 T210D
Plasmid#68272Purposebacterial expression of Plk1 T210D mutantDepositorInsertPLK1 (PLK1 Human)
UseTagsFlag and Mxe intein/chitin binding domainExpressionBacterialMutationT210DPromoterT7Available sinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCS2 Flag Smad3 S213A
Plasmid#27116DepositorInsertSmad3 (SMAD3 Human)
UseXenopus/mammalian/avian/zebrafishTagsFlagExpressionMutationS213APromoterAvailable sinceJan. 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO/intron/FlagCter
Plasmid#113548PurposeMammalian expression plasmid that can be used with the Flp-In T-REx system.DepositorInsertFlag
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVX-IRES-tdTomato-FlagAkt2W80A
Plasmid#64834PurposecDNA of Akt2 with point mutation from Trp to Ala at position 80. This mutation confers resistance to MK-2206 drug.DepositorInsertAkt2 (Akt2 Mouse)
UseLentiviralTags3xFlagExpressionMammalianMutationPoint mutation Trp 80 to AlaPromoterCMVAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQCXIH-Flag Smad3 EPSM
Plasmid#27026DepositorInsertSmad3 (SMAD3 Human)
UseRetroviralTagsFlagExpressionMammalianMutationT179V S204A S208A S213APromoterAvailable sinceDec. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
Flag-TRIM24-C840W shRescue
Plasmid#28137DepositorInsertTRIM24 (TRIM24 Human)
UseTagsFLAGX2ExpressionMammalianMutationC840Wmutated at aa 308 and 309 for shRNA rescue; …PromoterAvailable sinceFeb. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag MFF iso4 WT
Plasmid#74390PurposeFlag tagged human MFF isoform 4DepositorInsertFlag-MFF (MFF Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceApril 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-HA-CAD S1900A
Plasmid#46240PurposeFLAG-HA-CAD with S1900 mutated to alanineDepositorInsertcarbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad Mouse)
UseTagsFLAG and HAExpressionMammalianMutationSerine 1900 changed to Alanine (S1900A)PromoterCMVAvailable sinceNov. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Flag-YAP-S61A
Plasmid#33087DepositorInsertYAP (YAP1 )
UseTagsFlagExpressionMammalianMutationS61APromoterCMVAvailable sinceMarch 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-FLAG-Dicer-delDUF
Plasmid#51365DepositorInsertDicer (DICER1 Human)
UseTagsFLAGExpressionMammalianMutationFrom N terminus to DUF domain deletionPromoterCMVAvailable sinceMarch 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-bleMX6-P3nmt1-3FLAG
Plasmid#19351DepositorInsertbleMX6
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 25, 2008AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-bleMX6-P41nmt1-3FLAG
Plasmid#19352DepositorInsertbleMX6
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 25, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLJM60 FLAG NUFIP1 W40A
Plasmid#110951Purposeexpresses mammalian FLAG-NUFIP1 W40ADepositorInsertNUFIP1 W40A (NUFIP1 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationchanged Tryptophan 40 to alaninePromoterCMVAvailable sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A-3xFlag-S242R-MEFV
Plasmid#167020PurposeGateway entry plasmid with 3xFlag-pyrin (S242R/PAAND variant)DepositorInsertMEFV (MEFV Human)
UseGateway entry vectorTags3xFlagExpressionMutationserine 242 in arginine (S242R)PromoterAvailable sinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only