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Plasmid#157724Purposemammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp16 under control of a tetracycline-inducible promoterDepositorInsertFH-Nsp16 (ORF1ab SARS-CoV-2)
UseTagsFlag-His8ExpressionMammalianMutationcodon optimizedPromoterAvailable sinceAug. 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
Zhou-COVID-19-Spike
Plasmid#161029PurposeExtracellular domain of SARS-CoV-2 spike protein that has been engineered so that it assembles pseudo virus more efficiently. Also contains portions of VSV-G for production of psuedovirus.DepositorInsertEngineered COVID-19 Spike protein, VSV-G (S SAR-CoV-2, Vesicular stomatitis virus)
UseTagsExpressionMammalianMutationCodon optimizedPromoterAvailable sinceJan. 12, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV2-CAG-3xNLS-AausFP1
Plasmid#191096PurposeTo express a bright green FP to label eucaryotic cell nuclei. To be used in tissue-clearing methods and other fluorescent microscopy methodsDepositorInsert3xNLS-AausFP1
UseAAV and Synthetic BiologyTagsThree repeats of the nuclear localizzation seque…ExpressionMammalianMutationOptimized to Human codon usagePromoterCMV enhancer and CAGAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
NSP6-FR-2Strep
Plasmid#210317PurposeExpresses SARS-CoV-2 NSP6 in mammalian cellsDepositorInsertSARS-CoV-2 NSP6 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
UseTags2StrepExpressionMammalianMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Nsp15
Plasmid#165120Purposemammalian expression and localizationDepositorInsertNsp15 (ORF1ab codon optimized SARS-COV-2)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHS1801
Plasmid#205966PurposeJAGBLH010000220 Cas8-HNH full system (E. coli codon optimized) in pACYCDuet-1 with Lac promotersDepositorInsertCas8-HNH proteins and CRISPR array
UseTagsExpressionBacterialMutationPromoterLac (proteins), pJ23119 (crRNA)Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHS1232
Plasmid#205969PurposeMWEE01000013 Cas5-HNH operon (E. coli codon optimized) + CRISPR array in pACYCDuet-1 Lac promotersDepositorInsertCas5-HNH proteins and CRISPR array
UseTagsExpressionBacterialMutationPromoterLac (proteins), pJ23119 (crRNA)Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
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EGFP-Orf9b L52D
Plasmid#165129Purposemammalian expression and localizationDepositorInsertOrf9b L52D
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-FH-Nsp14
Plasmid#157718Purposemammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp14 under control of a tetracycline-inducible promoterDepositorInsertFH-Nsp14 (ORF1ab SARS-CoV-2)
UseTagsFlag-His8ExpressionMammalianMutationcodon optimizedPromoterAvailable sinceAug. 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5-FRT-TO-FH-Nsp12
Plasmid#157710Purposemammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp12 under control of a tetracycline-inducible promoterDepositorInsertFH-Nsp12 (ORF1ab SARS-CoV-2)
UseTagsFlag-His8ExpressionMammalianMutationcodon optimizedPromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
H513 px330.sgActin.UTR.2
Plasmid#138176PurposeCRISPR SONIC: Encodes codon-optimized SpCas9 and gRNA for targeting 3' UTR of β-actin.DepositorInsertsgActin (Actb Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-Nsp10-HF
Plasmid#157708Purposemammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp10 under control of a tetracycline-inducible promoterDepositorInsertNsp10-HF (ORF1ab SARS-CoV-2)
UseTagsHis8-FlagExpressionMammalianMutationcodon optimizedPromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
p3s-ABE7.10
Plasmid#113128Purposeexpressing ABE7.10 (TadA-TadA*7.10-nCas9-NLS-HA), human codon optimizedDepositorInsertABE 7.10
UseTagsHA epitope and SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Nsp12
Plasmid#165117Purposemammalian expression and localizationDepositorInsertNsp12 (ORF1ab codon optimized SARS-COV-2)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
NSP6-Flag
Plasmid#210345PurposeExpresses SARS-CoV-2 NSP6 fused with Flag in mammalian cellsDepositorInsertSARS-CoV-2 NSP6 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Human)
UseTagsFlagExpressionMammalianMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-Nsp5-HF
Plasmid#157687Purposemammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp5 under control of a tetracycline-inducible promoterDepositorInsertNsp5-HF (ORF1ab SARS-CoV-2)
UseTagsHis8-FlagExpressionMammalianMutationcodon optimizedPromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pcDNA5-FRT-TO-Nsp16-HF
Plasmid#157725Purposemammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp16 under control of a tetracycline-inducible promoterDepositorInsertNsp16-HF (ORF1ab SARS-CoV-2)
UseTagsHis8-FlagExpressionMammalianMutationcodon optimizedPromoterAvailable sinceAug. 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5-FRT-TO-FH-Nsp2
Plasmid#157683Purposemammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp2 under control of a tetracycline-inducible promoterDepositorInsertFH-Nsp2 (ORF1ab SARS-CoV-2)
UseTagsFlag-His8ExpressionMammalianMutationcodon optimizedPromoterAvailable sinceAug. 4, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits