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pEGFP-C1-BBS9

Plasmid

#218717

Purpose

Expresses N-terminally EGFP-tagged BBS9 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

Insert

BBS9 (BBS9 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

A455T (g1876a)

Promoter

CMV

Available since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pGWB405-TIC21-sGFP

Plasmid

#213865

Purpose

Binary vector for the expression of sGFP flanked with an N-terminal chloroplas inner envelope membrane transit peptide in plants .

Depositor

Yutaka Kodama

Article

Ichikawa et al Plant Direct. 2022 Nov 15;6(11):e462. do

Insert

chloroplast inner envelope membrane transit peptide (TIC21 Mustard Weed)

Use

Tags

Expression

Bacterial and Plant

Mutation

Promoter

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pB-Neo-rTOM20-TagBFP

Plasmid

#210332

Purpose

Overexpression of rTOM20

Depositor

Li Yu

Article

Jiao et al Cell. 2021 May 27;184(11):2896-2910.e13.

Insert

rTOM20 (Tomm20 Rat)

Use

Tags

TagBFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_EF1a_DIO_HA/FLAG_muMASS_LF

Plasmid

#213394

Purpose

Loss of function variant of uMASS_1 opioid sensor. AAV virus production for Cre dependent expression.

Depositor

Andre Berndt

Article

Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

Insert

uMASS_LF

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_EF1a_DIO_HA/FLAG_muMASS

Plasmid

#213393

Purpose

uMASS_1 opioid sensor. AAV Virus production for Cre dependent expression

Depositor

Andre Berndt

Article

Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

Insert

uMASS_1

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCF827-sgNT-1_U6-sgRNA-EFS-Cas9-P2A-mNeonGreen

Plasmid

#211693

Purpose

U6-sgRNA-EFS-Cas9-P2A-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgNT-1 guide RNA vector

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCF827-recipient_U6-sgRNA-EFS-Cas9-P2A-mNeonGreen

Plasmid

#211692

Purpose

U6-sgRNA-EFS-Cas9-P2A-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and guide RNA recipient vector

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCF827-sgCIDE-1_U6-sgRNA-EFS-Cas9-P2A-mNeonGreen

Plasmid

#211696

Purpose

U6-sgRNA-EFS-Cas9-P2A-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgCIDE-1 guide RNA vector

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCF827-sgNT-3_U6-sgRNA-EFS-Cas9-P2A-mNeonGreen

Plasmid

#211695

Purpose

U6-sgRNA-EFS-Cas9-P2A-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgNT-3 guide RNA vector

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCF827-sgCIDE-3_U6-sgRNA-EFS-Cas9-P2A-mNeonGreen

Plasmid

#211698

Purpose

U6-sgRNA-EFS-Cas9-P2A-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgCIDE-3 guide RNA vector

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCF827-sgCIDE-2_U6-sgRNA-EFS-Cas9-P2A-mNeonGreen

Plasmid

#211697

Purpose

U6-sgRNA-EFS-Cas9-P2A-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgCIDE-2 guide RNA vector

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCF826-sgNT-1_U6-sgRNA-EFS-Cas9-P2A-mCherry2

Plasmid

#211689

Purpose

U6-sgRNA-EFS-Cas9-P2A-mCherry2

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgNT-1 guide RNA vector

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCF826-sgCIDE-1_U6-sgRNA-EFS-Cas9-P2A-mCherry2

Plasmid

#211690

Purpose

U6-sgRNA-EFS-Cas9-P2A-mCherry2

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgCIDE-1 guide RNA vector

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCF826-sgCIDE-2_U6-sgRNA-EFS-Cas9-P2A-mCherry2

Plasmid

#211691

Purpose

U6-sgRNA-EFS-Cas9-P2A-mCherry2

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgCIDE-2 guide RNA vector

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_hsyn_HA_Flag-uMASS_LF

Plasmid

#209761

Purpose

AAV virus production for neuonal expression of uMASS (loss-of-function) under hSyn promoter

Depositor

Andre Berndt

Article

Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

Insert

uMASS_LF

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pCF826-recipient_U6-sgRNA-EFS-Cas9-P2A-mCherry2

Plasmid

#211688

Purpose

U6-sgRNA-EFS-Cas9-P2A-mCherry2

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and guide RNA recipient vector

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCF827-sgNT-2_U6-sgRNA-EFS-Cas9-P2A-mNeonGreen

Plasmid

#211694

Purpose

U6-sgRNA-EFS-Cas9-P2A-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgNT-2 guide RNA vector

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-dMASS_LF_WPRE

Plasmid

#209766

Purpose

AAV virus production for neuronal expression of dMASS_LF (loss-of-function) under hSyn promoter

Depositor

Andre Berndt

Article

Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

Insert

dMASS_LF

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV_hsyn_HA-Flag-uMASS_1_WPRE

Plasmid

#209760

Purpose

AAV virus production for neuronal expression of uMASS_1 under hSyn promoter

Depositor

Andre Berndt

Article

Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

Insert

uMASS_1

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAS_4xMmaPylT(UUA)_EF1_sfGFP150TAA

Plasmid

#174897

Purpose

ochre suppression reporter sfGFP150TAA expression, with MmaPylT(UUA) ochre suppressor tRNA cassette

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

sf GFP

Use

Tags

Expression

Mammalian

Mutation

150TAA in sfGFP

Promoter

EF1

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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