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Plasmid#64102PurposeTet inducible, Gateway adapted ROSA26 targeting VectorDepositorInsertrTTA (Gt(ROSA)26Sor Synthetic)
UseMouse Targeting; Tet inducibleTagsExpressionMammalianMutationPromoterROSA26 endogenousAvailable sinceDec. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFX-mCherry-KRasCT
Plasmid#175835PurposeExpress mCherry with plasma membrane-targeting sequence in mammalian cellsDepositorInsertmCherry with plasma membrane-targeting sequence
UseTagsC-terminal hypervariable region of K-Ras 4BExpressionMammalianMutationPromoterAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
6xHis-TEV-UBC-9
Plasmid#87055PurposeExpresses 6xHis-tagged ubc-9, with a TEV protease cleavage site between the tag and the ORFDepositorInsertUBC-9 (ubc-9 Nematode)
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7Available sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pL0_EF-H4
Plasmid#202178PurposeLevel 0 plasmid containing a terminator sequence from gene 34971 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.DepositorInsertH4 terminator
UseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 28, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL0_EF-fcpA
Plasmid#202164PurposeLevel 0 plasmid containing a terminator sequence from gene 18049 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.DepositorInsertfcpA terminator
UseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 28, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL0_AC-H4
Plasmid#202122PurposeLevel 0 plasmid containing the promoter from gene 34971 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.DepositorInsertH4 promoter
UseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 24, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL0_AC-P49
Plasmid#202120PurposeLevel 0 plasmid containing the promoter from gene 49202 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.DepositorInsertP49 promoter
UseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 24, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pU6-26 (GB1001)
Plasmid#68208PurposeProvides the A. thaliana U6-26 RNA polIII promoter as a level 0 GoldenBraid partDepositorInsertAtU6-26 promoter
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-V5-Sec61B
Plasmid#120244PurposeExpresses V5-tagged Sec61B in lentiviral vectorDepositorInsertSec61B (Sec61b Mouse)
UseLentiviralTagsV5ExpressionMutationPromoterEF1aAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
C-BLa 2.0
Plasmid#92060PurposeThe C-BLa 2.0 plasmid encodes a protein, where the TMD is connected to the C-terminus of the cytoplasmic domain of the ToxR protein and to the N-terminus of the C-terminal beta-lactamase fragment.DepositorInsertC-BLa 2.0 fusion protein
UseTagsFlag (DYKDHDG)ExpressionBacterialMutationPromoteraraBADAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-miniSOG-T2A-dTomato
Plasmid#193796PurposeExpresses miniSOG in the cytosol of mammalian cells, joined by a T2A cleavage site with dTomatoDepositorInsertminiSOG
UseAAVTagsFlag tagExpressionMammalianMutationPromoterCAGAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
Oct4-Cr1
Plasmid#66989PurposeExpresses gRNA that targets stop codon for OCT4DepositorInsertgRNA for Crispr targeting the OCT4 stop codon
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQE80L TriCatcher-RGDSP-MMP
Plasmid#112633PurposeTwo SpyCatchers linked by an elastin-like polypeptide with an RGDSP integrin-binding site, an MMP-cleavable sequence and a C-terminal SnoopCatcherDepositorInsertTriCatcher-RGDSP-MMP
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationcentral matrix metalloproteinase-cleavable sequen…PromoterT5Available sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag Jnk1b2
Plasmid#13752DepositorInsertJnk1b2 (MAPK8 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pEGB Tnos:NptII:Pnos (GB0226)
Plasmid#68212PurposeTranscriptional unit for kanamycin resistance gene plant expressionDepositorInsertNptII
UseSynthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterPnosAvailable sinceOct. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGST1-GGA3-VHS
Plasmid#44420DepositorInsertGGA3-VHS (GGA3 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJuly 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
HSC1-GiP
Plasmid#58254PurposeThis self-inactivated retroviral vector has an internal human EF1alpha promoter driving the expression of a bicistronic EGFP ires Puromycin cassetteDepositorInsertEnhanced Green Fluorescence Protein, Puromycin resistance gene
UseRetroviralTagsExpressionMammalianMutationPromoterHuman EF1-alphaAvailable sinceOct. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAC-mCherry2-G4
Plasmid#164167PurposeExpresses mCherry2 under the control of CMV promoter, basic vector for AAV, pAAV-CMV backbone, multiclonig site at C terminalDepositorInsertmCherry2
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEasyG1_zeo
Plasmid#184910PurposeTemplate to generate via PCR a single gRNA for expression in S. cerevisiaeDepositorInsertgRNA scaffold
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-BD-cop1at(335-675)
Plasmid#44973DepositorInsertBD-COP1 (COP1 Mustard Weed)
UseTagsGAL4 DNA-binding domainExpressionMammalianMutationdeleted amino acids 1-334PromoterpCMVAvailable sinceJune 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHsp18.2 (GB0153)
Plasmid#68171PurposeProvides the Arabidopsis thaliana heat shock protein 18.2 promoter as a level 0 GoldenBraid partDepositorInsertAtHsp18.2 promoter
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA-FLAG-Bbs4
Plasmid#128868PurposeFor mammalian expression of mouse Bbs4DepositorInsertBbs4 (Bbs4 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
mEos3-Rac1
Plasmid#92039PurposeExpresses mEos3-Rac1 fusion proteinDepositorInsertRAC1 (RAC1 Human)
UseTagsmEos3ExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceJune 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHsp70B (GB0155)
Plasmid#68172PurposeProvides the Arabidopsis thaliana heat shock protein 70B promoter as a level 0 GoldenBraid partDepositorInsertAtHsp70B promoter
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pCDNA3 Flag Jnk2b2
Plasmid#13757DepositorInsertJnk2b2 (MAPK9 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMXs-EGFP-ERas-IP
Plasmid#13826DepositorInsertERas (Eras Mouse)
UseRetroviralTagsEGFPExpressionMammalianMutationWTPromoterAvailable sinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRSET-TEV-12His
Plasmid#137032Purposeempty vector; C-terminal TEV-protease-cleavable His12; T7 promoter; BseRI cloning sites (e.g., In-Fusion compatible)DepositorTypeEmpty backboneUseTagsHis12 (TEV protease cleavable)ExpressionBacterialMutationPromoterT7Available sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pET28a-Hook3 aa 1-160-GCN4
Plasmid#74608PurposeExpresses artificially dimerized Hook domain of Hook3 in bacteria for purificationDepositorInserthuman Hook3 amino acids 1-160 (HOOK3 Human)
UseTags6x His, GCN4, Strep II, and superfolder GFPExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Akt2(T309A)
Plasmid#86625PurposeExpresses eGFP-tagged human Akt2(T309A) phosphomutantDepositorInsertAkt2(T309A) (AKT2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
3535 pET15b Bid p22
Plasmid#8784DepositorInsertBid p22 (Bid Mouse)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJuly 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
Dll1ICD-HPC4 pBacPAK8-3
Plasmid#17333DepositorInsertDelta-like 1 (Dll1 Mouse)
UseTagsHPC4ExpressionInsectMutationPromoterAvailable sinceFeb. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pDelila (GB0079)
Plasmid#68200PurposeProvides the CDS of the Antirrhinum majus Delila transcription factor as a level 0 GoldenBraid partDepositorInsertDelila coding region
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRosea (GB0026)
Plasmid#68194PurposeProvides the CDS of the Antirrhinum majus Rosea transcription factor as a level 0 GoldenBraid partDepositorInsertA. majus Rosea coding region
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSPORT1[NheI/glGFP/bglobin3'-UTR/Ins2]
Plasmid#74101Purposevector bearing an NheI restriction site for the two-step cloning method in Wang and Szaro, Dev Biol, 2015DepositorInsertsGreen Lantern Green Fluorescent Protein
Rabbit beta 1-globin gene 3'UTR
UseTagsExpressionMutationPromoterAvailable sinceMay 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR- flag-Lag16-Intb2
Plasmid#205203PurposeThis vector encodes of the synCAM (Intb2) and GFP nanobody LAG16DepositorInsertsynCAM Intb2 and Lag16 anti-GFP nanobody
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
mEos3-dnRac1
Plasmid#92040PurposeExpresses mEos3 fusion with dnRac1 (T17N).DepositorInsertRAC1 (RAC1 Human)
UseTagsmEos3ExpressionBacterial, Insect, and Mamm…MutationT17N (dominant negative)PromoterCMVAvailable sinceJune 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
5HT6-YFP-PIPK(D253A)
Plasmid#96811Purposekinase-dead control for 5HT6-YFP-PIPKDepositorInsert5HT6-YFP-PIPK(D253A)
UseTagsYFPExpressionMammalianMutationD253A inactivates kinase activity of PIPKPromoterAvailable sinceSept. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG Fzd7 GFP
Plasmid#66106Purposeexpression of chicken Fzd7 GFPDepositorInsertFzd7 (FZD7 Chicken)
UseChicken expressionTagsEGFPExpressionMutationPromoterCAGAvailable sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
p414GPD-3xFLAG-Nop4 WT
Plasmid#169261PurposeS. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-Nop4 WTDepositorInsertNop4 WT (NOP4 Budding Yeast)
UseTags3xFLAGExpressionYeastMutationPromoterGPDAvailable sinceDec. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag BimL (AA)
Plasmid#24240DepositorInsertBim L(AA) (BCL2L11 Human)
UseTagsFlagExpressionMammalianMutationchanged Threonine 56 to Alanine changed Serine …PromoterAvailable sinceMay 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag Jnk2b1
Plasmid#13756DepositorInsertJnk2b1 (MAPK9 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCMV4-p100SS/AA (SS866/870AA)
Plasmid#27555DepositorInsertp100 (NFKB2 Human)
UseTagsExpressionMammalianMutationSerines 866 and 870 mutated to AlaninesPromoterAvailable sinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CαN (K29,30A)-mVenus
Plasmid#168485PurposeMammalian expression of the K29,30A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.DepositorInsertthe K29,30A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEntry Tshld(FKBP F36V)-GFP
Plasmid#53260PurposepEntry Tshld(FKBP F36V)-GFP is a product of BP recombination reaction between attB flanked Tshld(F36V) system-encoded PCR product and pDonor 201 plasmid.DepositorInsertTshld(FKBP F36V)-GFP
UseTagsEGFP, Flag, and HAExpressionMutationFKBP F36VPromoterAvailable sinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRenilla (GB0056)
Plasmid#68198PurposeProvides the CDS of the Renilla protein as a level 0 GoldenBraid partDepositorInsertRenilla coding region
UseLuciferase and Synthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-P2X2-CFP
Plasmid#22401DepositorInsertATP-gated P2X channel 2 (P2rx2 Rat)
UseTagsCFPExpressionMammalianMutationPromoterAvailable sinceDec. 10, 2009AvailabilityAcademic Institutions and Nonprofits only -
3495 pcDNA3 Bid p15
Plasmid#8780DepositorInsertBid p15 (Bid Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 6, 2005AvailabilityAcademic Institutions and Nonprofits only