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pCIneo-CAA-RLuc-6xbsMS2-A95-Malat_AC

Plasmid

#148425

Purpose

Mammalian Expression of 6xbsMS2-A95-Malat

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

6xbsMS2-A95-Malat

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-GQ-RLuc-6xbsMS2-A95-Malat_AC

Plasmid

#148426

Purpose

Mammalian Expression of 6xbsMS2-A95-Malat

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

6xbsMS2-A95-Malat

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-HsNot6-159-557_AF

Plasmid

#148657

Purpose

Bacterial Expression of HsNot6_159-557

Depositor

Elisa Izaurralde

Article

Chen et al Nucleic Acids Res. 2021 Jun 21;49(11):64

Insert

HsNot6_159-557 (CNOT6 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-HsNot6-D491A_AF

Plasmid

#148659

Purpose

Bacterial Expression of HsNot6-D491A

Depositor

Elisa Izaurralde

Article

Chen et al Nucleic Acids Res. 2021 Jun 21;49(11):64

Insert

HsNot6-D491A (CNOT6 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-HsNot6-D491N_AF

Plasmid

#148660

Purpose

Bacterial Expression of HsNot6-D491N

Depositor

Elisa Izaurralde

Article

Chen et al Nucleic Acids Res. 2021 Jun 21;49(11):64

Insert

HsNot6-D491N (CNOT6 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-HsNot6-R65E_AF

Plasmid

#148663

Purpose

Bacterial Expression of HsNot6-R65E

Depositor

Elisa Izaurralde

Article

Chen et al Nucleic Acids Res. 2021 Jun 21;49(11):64

Insert

HsNot6-R65E (CNOT6 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-HsNot6-R87T_AF

Plasmid

#148665

Purpose

Bacterial Expression of HsNot6-R87T

Depositor

Elisa Izaurralde

Article

Chen et al Nucleic Acids Res. 2021 Jun 21;49(11):64

Insert

HsNot6-R87T (CNOT6 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pPW3756 : CMVd3-ERmembrane-HaloTag-KKMP

Plasmid

#185678

Purpose

Low-level transient expression of a single HaloTag protein with a C-terminal KKMP ER retention signal, targeted to the ER membrane.

Depositor

Peter Walter

Article

Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

Insert

ERmembrane-HaloTag-KKMP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pQE80l ACA

Plasmid

#196471

Purpose

Protein expression of SpyTag-ELP-CarHC-ELP-SpyTag

Depositor

Fei Sun

Article

Wang et al Proc Natl Acad Sci U S A. 2017 Jun 6;114

Insert

ACA

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pENmRFPCNAL2 pc1054

Plasmid

#167567

Purpose

The fusion protein contains a SV40 NLS followed by mRFP, linker and human PCNA.

Depositor

Cristina Cardoso

Article

Sporbert et al Nucleic Acids Res. 2005 Jun 21;33(11):35

Insert

Proliferating Cell Nuclear Antigen (PCNA Human)

Use

Tags

mRFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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RCAS myr-c-P3k

Plasmid

#196373

Purpose

Oncogenic with addition of a myristylation signal and expresses in avian cells

Depositor

Peter Vogt

Article

Chang et al Science. 1997 Jun 20;276(5320):1848-50.

Insert

Myr-c-P3k-FLAG

Use

Retroviral; Avian

Tags

flag and myristoylation

Expression

Mutation

Promoter

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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Rtn2b-R60f-BFP

Plasmid

#186608

Purpose

Encodes human Reticulon 2 disease mutant R60f fused to BFP

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Human Reticulon 2 mutant R60f (RTN2 Human)

Use

Tags

BFP

Expression

Mammalian

Mutation

c.178insC (R60f)

Promoter

CMV

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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Rtn2b-mNeon

Plasmid

#186598

Purpose

Encodes human full-length Reticulon 2 isoform B fused to mNeonGreen

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Human Reticulon 2 isoform B (RTN2 Human)

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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Rtn2b-mCherry

Plasmid

#186600

Purpose

Encodes human full-length Reticulon 2 isoform B fused to mCherry

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Human Reticulon 2 isoform B (RTN2 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_Cep41-STf

Plasmid

#182010

Purpose

CMV overexpression of SNAP-tag fast in mammalian cell lines associated to microtubules

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

Cep41-STf

Use

Tags

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_H2B-STf

Plasmid

#181998

Purpose

CMV overexpression of SNAP-tag fast fusion to H2B for nuclear localization in mammalian cell lines

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

H2B-STf

Use

Tags

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_[Cox8a]x2_STf

Plasmid

#182003

Purpose

CMV overexpression of SNAP-tag fast in the mitochondrial matrix of mammalian cell lines

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

[Cox8a]x2_STf

Use

Tags

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_CalR_STf_KDEL

Plasmid

#182004

Purpose

CMV overexpression of SNAP-tag fast in the endoplasmic reticulum of mammalian cell lines

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

CalR_STf_KDEL

Use

Tags

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_b4g-STf

Plasmid

#182005

Purpose

CMV overexpression of SNAP-tag fast in the golgi apparatus of mammalian cell lines

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

b4g-STf

Use

Tags

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-BLZinCh-P40

Plasmid

#188057

Purpose

Expression of BLZinCh-P40, a dual read-out BRET-FRET sensor for intracellular Zn2+ imaging, in mammalian cells

Depositor

Maarten Merkx

Article

Michielsen et al ACS Chem Biol. 2022 Jun 17;17(6):1567-15

Insert

BLZinCh-P40

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV promotor

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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