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Showing: 3381 - 3400 of 3890 results

  1. pRL_CT_GI_del1

    Plasmid
    #98744
    Purpose
    For red/far-red light-regulated gene expression in Saccharomyces cerevisiae. Integrates into the yeast ura3-52 locus. Encodes constitutively expressed PhyBNT-synTALE-DBD and PIF3-NLS-VP64AD fusions.
    Depositor
    Bernd Müller-Röber
    Article
    Hochrein et al Nucleic Acids Res. 2017 Sep 6;45(15):919
    Inserts
    phytochrome interacting factor 3 (PIF3 Mustard Weed)
    phytochrome B (PHYB Mustard Weed)
    Use
    Synthetic Biology
    Tags
    SV40-NLS, VP64-AD, and synTALE-DBD
    Expression
    Yeast
    Mutation
    mutated nucleotide 576 C to A, deleted amino acid…
    Promoter
    FBA1 and TDH3
    Available since
    Aug. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98744-plasmid-map-sequence-id-189858

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  4. pDONR207 SARS-CoV-2 NSP1 Delta RC

    Plasmid
    #164522
    Purpose
    Gateway-compatible Entry vector, with insert of mutated NSP1 CDS from SARS-CoV-2 isolate Wuhan-Hu-1 (amino acid substitutions K164A/H165A)
    Depositor
    Mitchell Guttman
    Article
    Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.
    Insert
    NSP1 Delta RC (ORF1ab )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Insert of NSP1 gene's CDS from SARS-CoV-2 is…
    Promoter
    Available since
    Feb. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    164522-plasmid-map-sequence-id-324313

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  6. pMIG-AVITEV- hNFATc1/aA

    Plasmid
    #74057
    Purpose
    retroviral expression plasmid for human NFATc1/aA with N-terminal BirA-biotinylation signal and TEV celavage site
    Depositor
    Ria Baumgrass
    Article
    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.
    Insert
    human NFATc1, isoform alpha-A (NFATC1 Human)
    Use
    Retroviral
    Tags
    AVI-TEV
    Expression
    Mammalian
    Mutation
    mutation G1157A [R235Q] and the silent mutation C…
    Promoter
    pMSCV-LTRs
    Available since
    June 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    74057-plasmid-map-sequence-id-142263

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  7. pDONR207 SARS-CoV-2 NSP1 124A/125A

    Plasmid
    #164523
    Purpose
    Gateway-compatible Entry vector, with insert of mutated NSP1 CDS from SARS-CoV-2 isolate Wuhan-Hu-1 (amino acid substitutions R124A/K125A)
    Depositor
    Mitchell Guttman
    Article
    Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.
    Insert
    NSP1 124A/125A (ORF1ab )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Insert of NSP1 gene's CDS from SARS-CoV-2 is…
    Promoter
    Available since
    Feb. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    164523-plasmid-map-sequence-id-323651

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  9. pMIG-hNFATc2/C(RIT)-FLAG-AVITEV

    Plasmid
    #74053
    Purpose
    retroviral expression plasmid for human NFATc2/C (with RIT mutation abrogating AP1 binding) with C-terminal BirA-biotinylation signal and TEV celavage site
    Depositor
    Ria Baumgrass
    Article
    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.
    Insert
    human NFATc2, isoform C (NFATC2 Human)
    Use
    Retroviral
    Tags
    AVI-TEV and FLAG
    Expression
    Mammalian
    Mutation
    silent mutation A1723C in the sequence of NM_1730…
    Promoter
    pMSCV-LTRs
    Available since
    June 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    74053-plasmid-map-sequence-id-142273

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  10. pBabe-kappa-HA-dL5-myc-ADRB2

    Plasmid
    #101253
    Purpose
    Expresses HA-dL5(E52D)-cMyc N-terminal fusion of beta-2 adrenergic receptor in mammalian cells, MMLV retroviral plasmid (MBIC5, dL5**, FAP, ADRB2)
    Depositor
    Marcel Bruchez
    Article
    Perkins et al Biochemistry. 2018 Feb 6;57(5):861-871.
    Insert
    kappa-HA-dL5-myc-ADRB2 (ADRB2 Budding Yeast, Human)
    Use
    Retroviral
    Tags
    FAP-dL5 and HA epitope
    Expression
    Mammalian
    Mutation
    Promoter
    MMLV LTR
    Available since
    Oct. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    101253-plasmid-map-sequence-id-235286

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Showing: 3381 - 3400 of 3890 results