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pEGFP C1 SADBs
Plasmid#67154PurposepEGFP containing N-terminally EGFP Tagged mouse SADBsDepositorInsertBrsk1 (Brsk1 Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET21 E2F1
Plasmid#90313PurposeExpresses human E2F1 in bacteriaDepositorInsertE2F1 (E2F1 Human)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceAug. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDL023_Tub-rb_IgG_HC
Plasmid#193620Purposeα-Tubulin heavy chain variable region + rabbit heavy chain constant regions + exogenous N-term signal peptide. For co-expression with pDL024 in HEK293 cells.DepositorInsertAnti-Tubulin scFv heavy variable + rabbit constant chain
UseTagssignal peptideExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDL024_Tub-rb_IgG_LC
Plasmid#193621Purposeα-Tubulin light chain variable region + rabbit light chain constant region + exogenous N-term signal peptide. For co-expression with pDL023 in HEK293 cells.DepositorInsertAnti-Tubulin scFv light variable + rabbit constant chain
UseTagssignal peptideExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pX459-HypaCas9-TUBA1B_sgRNA
Plasmid#183889PurposepX459V2.0-HypaCas9 plasmid with TUBA1B sgRNA for N-terminal tagging of alpha-tubulin in human cells.DepositorInsertTUBA1B sgRNA spacer (TUBA1B Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX459-HypaCas9-mR2-TUBA1B_sgRNA
Plasmid#183888PurposepX459V2.0-HypaCas9 plasmid with mRuby2 reporter and TUBA1B sgRNA for N-terminal tagging of alpha-tubulin in human cells.DepositorInsertTUBA1B sgRNA spacer (TUBA1B Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS/U6/RNAi SadBs shRNA mouse
Plasmid#67152PurposepBS/U6 containing shRNA to mouse SADBsDepositorInsertBRSK1 (Brsk1 Mouse)
UseTagsExpressionMammalianMutationPromoterU6Available sinceNov. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJS278
Plasmid#134139PurposeBacterial expression of human Ska1 microtubule binding domain (MTBD)DepositorInsertHis-Precission-human Ska1 MTBD (SKA1 Human)
UseTagsExpressionBacterialMutationaa 132-255, R155APromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIW040
Plasmid#134127PurposeBacterial expression of human Ska1 microtubule binding domain (MTBD)DepositorInsertHis-Precission-human Ska1 MTBD (SKA1 Human)
UseTagsExpressionBacterialMutationaa 132-255 R236APromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIW039
Plasmid#134126PurposeBacterial expression of human Ska1 microtubule binding domain (MTBD)DepositorInsertHis-Precission-human Ska1 MTBD (SKA1 Human)
UseTagsExpressionBacterialMutationaa 132-255 and K203A K206APromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIW028
Plasmid#134125PurposeBacterial expression of human Ska1 microtubule binding domain (MTBD)DepositorInsertHis-Precission-human Ska1 MTBD (SKA1 Human)
UseTagsExpressionBacterialMutationaa 132-255 and K183A K184APromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
MTK3b_002
Plasmid#123783PurposeEncodes human alpha tubulin 1 as a Type 3b part to be used in the MTK systemDepositorInsertalpha tubulin 1b (human
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR KIF17 (1-738)
Plasmid#60406PurposeExpression of truncated form of human homodimeric kinesin-2 (KIF17) amino acids 1-738DepositorInsertKIF17 (KIF17 Human)
UseLentiviralTagsGB1, Strep-Tag, and sfGFPExpressionMammalianMutationTruncated version amino acids 1-738PromoterSFFVAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pEGFP C1 SADBs shRNA Resistant
Plasmid#67155PurposepEGFP containing N-terminally EGFP Tagged mouse SADBs resistant to an shRNADepositorInsertBrsk1 (Brsk1 Mouse)
UseTagsEGFPExpressionMammalianMutationnucleotide 294 C-T, nucleotide 297 C-GPromoterCMVAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSG5 N-HA SADBs shRNA resistant
Plasmid#67157PurposeN-terminally HA tagged mouse SADBs containing substitutions providing resistance to an shRNADepositorInsertBrsk1 (Brsk1 Mouse)
UseTagsHAExpressionMammalianMutationNucleotide 294 C-T Nucleotide 297 C-G (shRNA resi…PromoterSV40 promoterAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTER SADB shRNA Human
Plasmid#67151PurposeDoxycycline-regulated mammalian expression vector for expressing shRNA against human SADBDepositorInsertBRSK1 (BRSK1 Human)
UseRNAiTagsExpressionMammalianMutationPromoterH1 TetAvailable sinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-TUBG2
Plasmid#171966PurposeExpression in cell lines of the TUBG2 geneDepositorInsertTUBG2 (TUBG2 Human)
UseTagsExpressionMammalianMutationRemoval of the internal TUBG2-EcoRI site by chang…PromoterAvailable sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTER E2F1shRNA human
Plasmid#66883PurposeDoxycycline-regulated mammalian expression vector for expressing shRNA against E2F1DepositorInsertE2F transcription factor 1 (E2F1 Human)
UseRNAiTagsExpressionMammalianMutationPromoterH1 TetAvailable sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only
