-
Plasmid#113942PurposeConstitutive bacterial expression of red fluorescent Ca2+ sensor jRGECO1aDepositorInsertjRGECO1a
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pME-TagRFPt-NS
Plasmid#75343PurposeMultisite gateway entry clone for adding N-terminal fusions of TagRFPtDepositorInsertTagRFPt
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pME-BASU-NS
Plasmid#166565PurposeMultisite Gateway middle entry clone encoding BASU. Parton lab clone JRODepositorInsertBASU
UseMultisite gateway entry cloneTagsExpressionMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCSN074
Plasmid#166731PurposeSingle copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS, codon optimized for Saccharomyces cerevisiaeDepositorInsertdCas12a-NLS
UseCRISPRTagsSV40 NLSExpressionYeastMutationE925APromoterS. cerevisiae TEF1Available sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-P2A-APEX2-ChBP
Plasmid#108879PurposeExpression of APEX2 fused to a nanobody against mCherry (includes green cytoplasmic reporter)DepositorInsertEGFP-P2A-APEX2-ChBP
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-hygro-eGFP-HEC1-9D
Plasmid#114029PurposeInducible expression of eGFP-tagged HEC1-9D in FLPin Trex cell linesDepositorInserteGFP-HEC1 - 9D (NDC80) (NDC80 Human)
UseTagsExpressionMammalianMutationHec1 - 9DPromoterAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBAD-R-GECO1
Plasmid#113943PurposeBacterial expression of red fluorescent Ca2+ sensor R-GECO1DepositorInsertR-GECO1
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
acta1-mRuby2-dGBP
Plasmid#163855PurposeZebrafish muscle specific expression of mRuby2-dVHH[GFP]DepositorInsertdVHH[GFP]
UseIn vitro transcription of rnaTagsRuby2ExpressionMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBAD-CAR-GECO1
Plasmid#113939PurposeBacterial expression of red fluorescent Ca2+ sensor CAR-GECO1DepositorInsertCAR-GECO1
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCSN069
Plasmid#166728PurposeSingle copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to Mxi1, codon optimized for Saccharomyces cerevisiaeDepositorInsertdCas12a-Mxi1
UseCRISPRTagsMxi1ExpressionYeastMutationE925APromoterS. cerevisiae TEF1Available sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLys-M1 backbone
Plasmid#109386PurposeEmpty plasmid of the pLys-M1 construct. This is medium copy (p15A), CmRDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-TurboID-CaaX
Plasmid#163848PurposeIn vitro transcription of TurboID-CaaX. Parton lab clone JSUDepositorInsertTurboID
UseIn vitro transcription of rnaTagsCaaXExpressionMutationPromoterAvailable sinceSept. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pME-mKate2-NS
Plasmid#109729PurposeMultisite gateway vector for 5' tagging with mKate2DepositorInsertmKate2
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-hygro-eGFP-HEC1-8A/S55D
Plasmid#114032PurposeInducible expression of eGFP-tagged HEC1-8A/S55D in FLPin Trex cell linesDepositorInserteGFP-HEC1-8A/S55D (NDC80) (NDC80 Human)
UseTagsExpressionMammalianMutationHec1 - 8A/S55DPromoterAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
LV-TRE-p65 mouse MyoD-T2A-dsRedExpress2
Plasmid#60627PurposeExpresses p65 mouse MyoD and dsRedExpress2 in response to doxycyclineDepositorInsertp65 mouse MyoD
UseLentiviralTagsExpressionMutationPromoterTREAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pOZ-N-FH PSMD14
Plasmid#27500DepositorInsertPSMD14 (PSMD14 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationM1VPromoterAvailable sinceMarch 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSCKtheoRaj12m
Plasmid#69943PurposepSTC2,TheoHHAzRaj12mut,cisRaj12-sfGFP, kanRDepositorInsertpSCKtheoRaj12m
UseSynthetic Biology; Psc101 origin of replicationTagsExpressionMutationPromoterAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-CD44b-TurboID
Plasmid#163850PurposeIn vitro transcription of CD44b-TurboID. Parton lab clone JTJDepositorInsertCD44b (cd44b Zebrafish)
UseIn vitro transcription of rnaTagsTurboIDExpressionMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-BASU-EGFP
Plasmid#163845PurposeIn vitro transcription of BASU-EGFP. Parton lab clone JRRDepositorInsertBASU
UseIn vitro transcription of rnaTagsEGFPExpressionMutationPromoterAvailable sinceSept. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTub-alpha-1-mtGFP
Plasmid#138981PurposeNeuron-specific expression of membrane targeted EGFPDepositorInsertEGFP
UseTagsExpressionMammalianMutationEGFP (added membrane targeting domain from GAP43).PromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only