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Plasmid#78300PurposeFRET biosensor. Visualization of cytosolic FAK activity in live cellsDepositorInsertCytosolic FAK biosensor
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSH1/M-Fv-Casp3-E
Plasmid#15271DepositorInsertFKBP12(V36)-Caspase3 (CASP3 Human)
UseTagsHA epitope and Myristoylation-targeting domain c-…ExpressionMammalianMutationFKBP12(V36) F36->VPromoterAvailable sinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSH1/S-Fvls-p30Casp9-E
Plasmid#15272DepositorInsertFKBP12(V36)-p30Caspase9 (CASP9 Human)
UseTagsHA epitope and Myristoylation-targeting domain c-…ExpressionMammalianMutationFKBP12 F36 to VTruncated Caspase9 (135-416; lacki…PromoterAvailable sinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSH1/M-Fv'-Fvls-E
Plasmid#15273DepositorInsertFKBP12(V36) (FKBP1A Human)
UseTagsHA epitope and Myristoylation-targeting domain c-…ExpressionMammalianMutationFKBP12 F36-->V 1st FKBP uses "wobbled&qu…PromoterAvailable sinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
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Lyn FAK biosensor
Plasmid#78299PurposeFRET biosensor. Visualization of FAK activity at lipid rafts in live cellsDepositorInsertLyn Fak biosensor
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
KRas FAK biosensor
Plasmid#78301PurposeFRET biosensor. Visualization of FAK activity at plasma membrane outside lipid rafts in live cellsDepositorInsertKRas FAK biosensor
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-Shp2 T7DA/S189D SH2s
Plasmid#67577PurposeExpresses GST-tagged Shp2 T73D/S189D SH2 domains in bacteriaDepositorInsertSrc homology 2 domain-containing phosphatase 2
UseTagsGSTExpressionBacterialMutationDeleted amino acid residues 219-597, changed T73 …PromoterAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
FAT FAK biosensor
Plasmid#78303PurposeFRET biosensor. Visualization of FAK activity at focal adhesions in live cellsDepositorInsertFAT FAK biosensor
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-NCBD + ACTR
Plasmid#99335PurposeBacterial coexpression of CBP NCBD and NCoA3 binding domainsDepositorUseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
CmCitrine Lck w/ NosUTRs
Plasmid#66785Purposeexpression of fluorescent membranes in sea urchin primordial germ cellsDepositorInsertLck (LCK Human)
UseSea urchin, zebrafish, xenopusTags3' UTR from sea urchin Nanos, 5' UTR fr…ExpressionMutationTandem repeat of LCK the N-terminal membrane targ…PromoterT7Available sinceJuly 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX Fgr-SH2
Plasmid#46427DepositorInsertFgr (FGR Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 137-245PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
TFORF0918
Plasmid#142199PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNCOA1 (NCOA1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0919
Plasmid#142206PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNCOA1 (NCOA1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0920
Plasmid#143239PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNCOA1 (NCOA1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF3052
Plasmid#144528PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNCOA3 (NCOA3 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0917
Plasmid#142198PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNCOA3 (NCOA3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSH1/M-FGFR1-Fv-Fvls-E
Plasmid#15285DepositorInsertFGFR1 kinase, FKBP12v36 (Fgfr1 Mouse)
UseTagsHA epitope and Myristoylation-targeting domain c-…ExpressionMammalianMutationFGFR1 cytoplasmic domain FKBP12 F36VPromoterAvailable sinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSH1/M-FGFR2-Fv-Fvls-E
Plasmid#15286DepositorInsertFGFR2 kinase, FKBP12v36 (Fgfr2 Mouse)
UseTagsMyristoylation-targeting domain c-Src (14aa) and …ExpressionMammalianMutationFGFR2 cytoplasmic domain FKBP12 F36VPromoterAvailable sinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSH1/M-FGFR3-Fv-Fvls-E
Plasmid#15287DepositorInsertFGFR3 kinase, FKBP12v36 (Fgfr3 Mouse)
UseTagsMyristoylation-targeting domain c-Src (14aa) and …ExpressionMammalianMutationFGFR3 cytoplasmic domain only FKBP12 F36VPromoterAvailable sinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only