Addgene

We narrowed to 1,125 results for: smo

Showing: 321 - 340 of 1125 results

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36INS2-pBAD_FB

Plasmid

#78676

Purpose

Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. Has near identical expression as 36INS1-pBAD_AB [F:36INS2-pBAD:B]

Depositor

Douglas Densmore

Article

Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

Insert

Insulated pBAD Promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 1, 2016

Availability

Academic Institutions and Nonprofits only

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36INS2-pBAD_EB

Plasmid

#78675

Purpose

Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. Has near identical expression as 36INS1-pBAD_AB [E:36INS2-pBAD:B]

Depositor

Douglas Densmore

Article

Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

Insert

Insulated pBAD Promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 1, 2016

Availability

Academic Institutions and Nonprofits only

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36INS2-pBAD_AB

Plasmid

#78674

Purpose

Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. Has near identical expression as 36INS1-pBAD_AB [A:36INS2-pBAD:B]

Depositor

Douglas Densmore

Article

Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

Insert

Insulated pBAD Promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 1, 2016

Availability

Academic Institutions and Nonprofits only

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36INS1-pBAD_AB

Plasmid

#78670

Purpose

Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected.Modified from BBa_I13453 to adjust spacing in MoClo system. [A:36INS1-pBAD:B]

Depositor

Douglas Densmore

Article

Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

Insert

Insulated pBAD Promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 1, 2016

Availability

Academic Institutions and Nonprofits only

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DVK_FG

Plasmid

#66070

Purpose

MoClo Destination Vector: (based on pSB1K3) [F:LacZa:G]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Type

Empty backbone

Use

Synthetic Biology; Destination vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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DVK_GH

Plasmid

#66071

Purpose

MoClo Destination Vector: (based on pSB1K3) [G:LacZa:H]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Type

Empty backbone

Use

Synthetic Biology; Destination vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pCS4134

Plasmid

#131747

Purpose

Human reduced folate transporter 1 SLC19A1 for lentiviral transduction, copGFP transduction marker

Depositor

Christina Smolke

Article

Xiang et al Nat Commun. 2019 Sep 23;10(1):4327. doi:

Insert

Human reduced folate carrier 1 (SLC19A1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pSJ1256 (pFA6-link-yGFP1-10-CaURA3MX)

Plasmid

#86419

Purpose

C-terminal PCR tagging module for yeast codon optimized split GFP1-10

Depositor

Sue Jaspersen

Article

Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

Insert

GFP1-10

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pSJ1321 (pRS315-NOP1pr-GFP11-mCherry-PUS1)

Plasmid

#86413

Purpose

nuclear split GFP11 reporter with mCherry

Depositor

Sue Jaspersen

Article

Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

Insert

Nop1pr-GFP11-mCherry-PUS1

Use

Tags

GFP11 and mCherry

Expression

Yeast

Mutation

Promoter

NOP1

Available since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-3xFLAG-HSP27

Plasmid

#129229

Purpose

Encodes FLAG tagged human HSP27 for purification by GST affinity

Depositor

Kevin Janes

Article

Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

Insert

HSP27 (HSPB1 Human)

Use

Tags

FLAG and GST

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pSJ1568 (pRS315-NOP1pr-GFP11-mCherry-SCS2TM)

Plasmid

#86416

Purpose

ONM/ER split GFP11 reporter with mCherry

Depositor

Sue Jaspersen

Article

Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

Insert

NOP1pr-GFP11-mCherry-Scs2TM

Use

Tags

GFP11 and mCherry

Expression

Yeast

Mutation

Promoter

NOP1

Available since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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J23103_GB

Plasmid

#65991

Purpose

MoClo Basic Part: Constitutive promoter - Anderson series - low strength [G:J23103:B]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

Constitutive promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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J23107_GB

Plasmid

#65999

Purpose

MoClo Basic Part: Constitutive promoter - Anderson series - medium strength [G:J23107:B]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

Constitutive promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
J23116_GB

Plasmid

#66003

Purpose

MoClo Basic Part: Constitutive promoter - Anderson series - low strength [G:J23116:B]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

Constitutive promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
J23100_GB

Plasmid

#65983

Purpose

MoClo Basic Part: Constitutive promoter - Anderson series - high strength [G:J23100:B]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

Constitutive promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pSJ1602 (pRS315-NOP1pr-mCherry-SCS2TM-GFP11)

Plasmid

#86417

Purpose

luminal split GFP11 reporter with mCherry

Depositor

Sue Jaspersen

Article

Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

Insert

NOP1pr-mCherry-SCS2TM-GFP11

Use

Tags

GFP11 and mCherry

Expression

Yeast

Mutation

Promoter

NOP1

Available since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pSJ1679 (pRS315-NOP1pr-GFP11-PUS1)

Plasmid

#86414

Purpose

nuclear split GFP11 reporter without mCherry

Depositor

Sue Jaspersen

Article

Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

Insert

NOP1pr-GFP11-PUS1

Use

Tags

GFP11 and mCherry

Expression

Yeast

Mutation

Promoter

NOP1pr

Available since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pS1643 (pFA6-NATMX-CDC42pr-yGFP1-10)

Plasmid

#86420

Purpose

N-terminal PCR tagging cassette for yeast codon optimized split GFP1-10 with CDC42 promoter

Depositor

Sue Jaspersen

Article

Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

Insert

CDC42pr-GFP1-10

Use

Tags

GFP1-10

Expression

Yeast

Mutation

Promoter

Available since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pET-29b-3xGluGlu-c-jun-His6

Plasmid

#129227

Purpose

Encodes 3xGluGlu tagged human c-Jun for purification by nickel affinity

Depositor

Kevin Janes

Article

Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

Insert

cJun (JUN Human)

Use

Tags

6xHistidine

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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Sm-Myocardin Omni-pShuttle

Plasmid

#122122

Purpose

Expression of the smooth muscle version of myocardin

Depositor

Paul Herring

Article

Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

Insert

Myocardin (Myocd Mouse)

Use

Tags

Omni

Expression

Mammalian

Mutation

Promoter

Available since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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